Schematic representation of the association between metabolic rate and plasma levels of glucocorticoids and glucose. Green

Meta regression model testing the association between metabolic rate (MR) effect sizes and glucocorticoid effect sizes.

Forest plot showing the glucocorticoid (GC) effect sizes (Cohen’s D ± 95% CI) associated to experimental manipulations of metabolic rate, grouped by treatment group and study. Area of squares is proportional to the experiment sample size (1/s.e.).

Glucocorticoid effect size (Cohen’s D) increases with increasing metabolic rate effect size similarly in studies of mammals (open circles) and birds (closed circles). Area of dots is proportional to the experiment sample size (i.e. square root of the number of individuals in which GCs were measured).

Table showing the main effects of all variables considered (Metabolic Rate, Taxa, Time effect, Within-individual variation, Metabolic variable, and Treatment Type) to modulate glucocorticoid effect sizes across studies. Full models are shown in Table S4.

Study selection steps and number of studies found

Meta regression model (quantitative approach) testing the effect of (a) Taxa, (b) Before / after effect, (c) Experiment / control effect, (d) Use of Metabolic Rate or Heart Rate as metabolic variable and (e) Treatment type, on the association between metabolic rate (MR) and glucocorticoid effect sizes across studies.

Forest plot showing the metabolic rate (MR) effect sizes (Cohen’s D ± 95% CI) associated to experimental manipulations of metabolic rate, grouped by treatment group and study. Area of squares is proportional to the experiment sample size (1/s.e.).

Relationship between metabolic rate and glucocorticoid effect sizes (Cohen’s D) across studies. Panels show the association without ln-transforming metabolic rate effect sizes (left panel) and when ln-transforming them (right panel). Size of dots is proportional to the experiment sample size (i.e. square root of the number of individuals in which glucocorticoids were measured). Shaded areas represent 95% C.I. Note that the number of data points in the graph is higher than the number of studies, as some of the studies included multiple experimental treatments (Study ID was included as random factor in statistical analyses).

Relationship between metabolic rate and glucocorticoid effect sizes (Cohen’s D) across studies as a function of a) Before / after effect (open circles and dashed line for studies including a time effect; closed circles and continuous line for studies not including a time effect; see methods) and b) Experiment / control effect (open circles and dashed line for studies including within-individual variation; closed circles and continuous line for studies not including within-individual variation). Size of dots is proportional to the experiment sample size (i.e. square root of the number of individuals in which glucocorticoids were measured). Shaded areas represent 95% C.I. Note that the number of data points in the graph is higher than the number of studies, as some of the studies included multiple experimental treatments (Study ID was included as random factor in statistical analyses). Before / after effect and Experiment / control effect results should be interpreted with caution, as most studies do include within individual variation and do not include time effect.