Introduction

Receptor tyrosine kinases (RTK) are involved in wide range of developmental processes. In humans, the Anaplastic Lymphoma Kinase (ALK) RTK is expressed in the central and peripheral nervous system and its role as an oncogene in the childhood cancer neuroblastoma, which arises from the peripheral nervous system, is well described (Iwahara et al, 1997; Matthay et al, 2016; Umapathy et al, 2019; Vernersson et al, 2006).

In Drosophila melanogaster, ALK is expressed in the visceral mesoderm, central nervous system (CNS) and at neuromuscular junctions (NMJ). The critical role of Drosophila Alk and its ligand Jelly belly (Jeb) in the development of the embryonic visceral mesoderm has been extensively studied (Englund et al, 2003; Lee et al, 2003; Loren et al, 2003; Pfeifer et al, 2022; Popichenko et al, 2013; Schaub & Frasch, 2013; Shirinian et al, 2007; Varshney & Palmer, 2006; Wolfstetter et al, 2017). In the CNS, Alk signaling has been implicated in diverse functions, including targeting of photoreceptor axons in the developing optic lobes (Bazigou et al, 2007), regulation of NMJ synaptogenesis and architecture (Rohrbough & Broadie, 2010; Rohrbough et al, 2013) and mushroom body neuronal differentiation (Pfeifer et al., 2022). In addition, roles for Alk in neuronal regulation of growth and metabolism, organ sparing and proliferation of neuroblast clones, as well as sleep and long-term memory formation in the CNS have been reported (Bai & Sehgal, 2015; Cheng et al, 2011; Gouzi et al, 2011; Orthofer et al, 2020). The molecular mechanisms underlying these Alk-driven phenotypes are currently under investigation, and some potential molecular components of Drosophila Alk signaling in the larval CNS, such as the protein tyrosine phosphatase Corkscrew (Csw), have been identified in recent BioID-based in vivo proximity labeling analyses (Uckun et al, 2021).

In this work, we aimed to capture Alk-signaling dependent transcriptional events in the Drosophila larval CNS using Targeted DamID (TaDa) that profiles RNA polymerase II (Pol II) occupancy. TaDa employs a prokaryotic DNA Adenine methyltransferase (Dam) to specifically methylate adenines within GATC sequences present in the genome, creating unique GA^meTC marks. In TaDa, Dam is fused to Pol II resulting in GA^meTC marks on sequences adjacent to the Pol II binding site and can be combined with the Gal4/UAS system to achieve cell-type specific transcriptional profiling (Southall et al, 2013). Tissue specific TaDa analysis of Alk signaling, while genetically manipulating Alk signaling output, has previously been used to identify Alk transcriptional targets in the embryonic visceral mesoderm, such as the transcriptional regulator Kahuli (Kah) (Mendoza-Garcia et al, 2021). Here, we employed this strategy to identify Alk transcriptional targets in Drosophila larval brain tissue. These Alk TaDa identified transcripts were enriched in neuroendocrine cells. Further integration with bulk RNA-seq datasets generated from Alk gain-of-function and loss-of-function alleles, identified the uncharacterized neuropeptide precursor (CG4577), as an Alk target in the Drosophila brain, that we have named Sparkly (Spar) based on its protein expression pattern. Spar is expressed in a subset of Alk-expressing cells in the central brain and ventral nerve cord, overlapping with the expression pattern of neuroendocrine specific transcription factor Dimmed (Dimm) (Hewes et al, 2003). Further, using genetic manipulation of Alk we show that Spar levels in the CNS respond to Alk signaling output, validating Spar as a transcriptional target of Alk. Spar mutant flies showed significant reduction in life-span, and behavioral phenotypes including defects in activity, sleep, and circadian rhythm. Notably, Alk loss-of-function alleles displayed similar behavioral defects, suggesting that Alk-dependant regulation of Spar in peptidergic neuroendocrine cells modulates activity and sleep/rest behavior. Interestingly, Alk and its ligand Alkal2 play a role in regulation of behavioral and neuroendocrine function in vertebrates (Ahmed et al, 2022; Bilsland et al, 2008; Borenas et al, 2021; Lasek et al, 2011a; Lasek et al, 2011b; Orthofer et al., 2020; Weiss et al, 2012; Witek et al, 2015). Taken together, our findings suggest an evolutionarily conserved role of Alk signaling in the regulation of neuroendocrine cell function and identify Spar as the first molecular target of Alk to be described in the regulation of activity and circadian clock in the fly.

Results

TaDa identifies Alk-regulated genes in Drosophila larval CNS

To characterize Alk transcriptional targets in the Drosophila CNS we employed Targeted DamID (TaDa). Briefly, transgenic DNA adenine methyl transferase (Dam) fused with RNA-Pol II (here after referred as Dam-Pol II) (Southall et al., 2013) (Fig. 1A-B), was driven using the pan neuronal C155-Gal4 driver. To inhibit Alk signaling we employed a dominant negative Alk transgene, which encodes the Alk extracellular and transmembrane domain (here after referred as UAS-Alk^DN) (Bazigou et al., 2007) (Fig. 1A). Flies expressing Dam-Pol II alone in a wild-type background were used as control. Expression of Dam-Pol II was confirmed by expression of mCherry, which is encoded by the primary ORF of the TaDa construct (Southall et al., 2013) (Fig. 1B, Fig. S1A-B’). CNS from third instar wandering larvae were dissected and genomic DNA was extracted, fragmented at GA^meTC marked sites using methylation specific DpnI restriction endonuclease. The resulting GATC fragments were subsequently amplified for library preparation and NGS sequencing (Fig. S1C). Bioinformatic data analysis was performed based on a previously described pipeline (Marshall & Brand, 2017; Mendoza-Garcia et al., 2021). Initial quality control analysis indicated comparable numbers of quality reads between samples and replicates, identifying >20 million raw reads per sample that aligned to the Drosophila genome (Fig. S1D). No significant inter-replicate variability was observed (Fig. S1E). Meta-analysis of reads associated with GATC borders showed a tendency to accumulate close to Transcription Start Sites (TSS) indicating the ability of TaDa to detect transcriptionally active regions (Fig. S1F). A closer look at the Pol II occupancy profile of Alk shows a clear increase in Pol II occupancy from Exon 1 to Exon 7 (encoding the extracellular and transmembrane domain) in Alk^DN samples reflecting the expression of the dominant negative Alk transgene (Fig. S1G).

Figure 1.

To detect differential Pol II occupancy between Dam-Pol II control (C155 Gal4>UAS-LT3-Dam::Pol ll) and UAS-Alk^DN (C155-Gal4>UAS-LT3-Dam::Pol II; UAS-Alk^DN) samples, neighbouring GATC associated reads, maximum 350 bp apart (median GATC fragment distance in the Drosophila genome) were clustered in peaks (Tosti et al, 2018). More than 10 million reads in both control and Alk^DN samples were identified as GATC associated reads (Fig. S1D’), and those loci displaying differential Pol II occupancy were defined by logFC and FDR (as detailed in materials and methods). Greater than 50% of aligned reads were in promoter regions, with 33.55% within a 1 kb range (Fig. 1C, Table S1).

To further analyse transcriptional targets of Alk signaling we focused on loci exhibiting decreased Pol II occupancy when compared with controls, identifying 2502 loci with logFC<-2, FDR<0.05 (Fig. 1D, Table S1). Genes previously known to be associated with Alk signaling, such as kirre, RhoGAP15B, C3G, mib2 and mamo, were identified among downregulated loci (Fig. 1D). We compared CNS TaDa Alk targets with our previously published embryonic visceral mesoderm TaDa datasets that were derived under similar experimental conditions (Mendoza-Garcia et al., 2021) and found 775 common genes (Fig. 1E, Table S1). Gene ontology (GO) analysis identified GO terms in agreement with previously reported Alk functions in the CNS (Bai & Sehgal, 2015; Bazigou et al., 2007; Cheng et al., 2011; Gouzi et al., 2011; Orthofer et al., 2020; Pfeifer et al., 2022; Rohrbough & Broadie, 2010; Rohrbough et al., 2013; Woodling et al, 2020) such as axon guidance, determination of adult lifespan, nervous system development, regulation of gene expression, mushroom body development, behavioral response to ethanol and locomotor rhythm (Fig. 1F). Many of the differentially regulated identified loci have not previously been associated with Alk signaling and represent candidates for future characterisation.

TaDa targets are enriched for neuroendocrine transcripts

To further characterise Alk-regulated TaDa loci, we set out to examine their expression in scRNA-seq data from wild-type third instar larval CNS (Pfeifer et al., 2022). Enrichment of TaDa loci were identified by using AUCell, an area-under-the curve based enrichment score method, employing the top 500 TaDa hits (Aibar et al, 2017) (Fig. 2A-B, Table S1; Fig. S2). This analysis identified 786 cells (out of 3598), mainly located in a distinct cluster of mature neurons (Fig. 2B, red circle; Fig. 2C). This cluster was defined as neuroendocrine cells based on canonical markers, such as the neuropeptides Lk (Leucokinin), Nplp1 (Neuropeptide-like precursor 1), Dh44 (Diuretic hormone 44), Dh31 (Diuretic hormone 31), sNPF (short neuropeptide F), AstA (Allatostatin A), and the enzyme Pal2 (Peptidyl-α-hydroxyglycine-α-amidating lyase 2) as well as Eip74EF (Ecdysone-induced protein 74EF), and Rdl (resistance to dieldrin)(Guo et al, 2019; Huckesfeld et al, 2021; Takeda & Suzuki, 2022; Torii, 2009) (Fig. 2D-F). Overall, the TaDa-scRNAseq data integration analysis suggests a role of Alk signaling in regulation of gene expression in neuroendocrine cells.

Figure 2.

To further explore the observed enrichment of Alk-regulated TaDa loci in neuroendocrine cells, we used a Dimmed (Dimm) transcription factor reporter (Dimm Gal4>UAS-GFPcaax), as a neuroendocrine marker (Park et al, 2008), to confirm Alk protein expression in a subset of neuroendocrine cells in the larval central brain and ventral nerve cord (Fig. 2G). This could not be confirmed at the RNA level, due to low expression of dimm in both our and publicly available single cell RNASeq datasets (Brunet Avalos et al, 2019; Michki et al, 2021; Pfeifer et al., 2022).

Multi-omics integration identifies CG4577 as an Alk transcriptional target

Loci potentially subject to Alk-dependent transcriptional regulation were further refined by integration of the Alk-regulated TaDa dataset with previously collected RNA seq datasets (Fig. 3A). Specifically, w¹¹¹⁸ (control), Alk^Y1355S (Alk gain-of-function) and Alk^ΔRA (Alk loss-of-function) RNA-seq datasets (Pfeifer et al., 2022) were compared to identify genes that exhibited both significantly increased expression in Alk gain-of function conditions (w¹¹¹⁸ vs Alk^Y1355S) and significantly decreased expression in Alk loss-of-function conditions (w¹¹¹⁸ vs Alk^ΔRA and control vs Alk^DN). Finally, we positively selected for candidates expressed in Alk positive cells in our scRNA-seq dataset. Notably, the only candidate which met these stringent criteria was CG4577, which encodes an uncharacterised putative neuropeptide precursor (Fig. 3B). CG4577 exhibited decreased Pol II occupancy in Alk^DN samples (Fig. 3C), and CG4577 transcripts were upregulated in Alk^Y1355S gain-of-function conditions and downregulated in Alk^ΔRA loss-of-function conditions (Fig. 3D). In agreement with a potential role as a neuropeptide precursor, expression of CG4577 was almost exclusively restricted to neuroendocrine cell clusters in our scRNA-seq dataset (Fig. 3E). Examination of additional publicly available first instar larval and adult CNS scRNAseq datasets (Brunet Avalos et al., 2019; Davie et al, 2018) confirmed the expression of CG4577 in Alk-expressing cells (Fig. S3A-B). CG4577-RA encodes a 445 amino acid prepropeptide with a 27 aa N-terminal signal peptide sequence as predicted by SignalP-5.0) (Fig. 3F) (Almagro Armenteros et al, 2019). Analysis of CG4577-PA at the amino acid level identified a high percentage of glutamine residues (43 of 445; 9%), including six tandem glutamine repeats (amino acids 48-56, 59-62, 64-71, 116-118, 120-122 and 148-150) of unknown function as well as a lack of cysteine residues. The preproprotein has an acidic pI of 5.1 and carries a net negative charge of 6. Several poly- and di-basic prohormone convertase (PC) cleavage sites were also predicted (KR, KK, RR, RK) (Pauls et al, 2014; Southey et al, 2006; Veenstra, 2000) (Fig. 3F). Since the propeptide does not contain cysteine residues it is unable to form intracellular or dimeric disulfide bridges. A second transcript, CG4577-RB, encodes a 446 amino acid protein with only two amino acid changes (Fig. S3C). Phylogenetic analysis of CG4577 relative to known Drosophila neuropeptide precursors failed to identify strong homology in keeping with the known low sequence conservation of neuropeptide prepropeptides outside the bioactive peptide stretches. However, we were also unable to find sequence homologies with other known invertebrate or vertebrate peptides. Next, we searched for CG4577 orthologs across Metazoa. We obtained orthologs across the Drosophilids, Brachyceran flies and Dipterans. No orthologs were found at higher taxonomic levels, suggesting that CG4577 either originated in Dipterans, or has a high sequence variability at higher taxonomic levels. To identify conserved peptide stretches indicating putative bioactive peptide sequences, we aligned the predicted aa sequences of the Dipteran CG4577 orthologs. This revealed several conserved peptide stretches (Fig. S4) framed by canonical prohormone cleavage sites that might represent bioactive peptide sequences. BLAST searches against these conserved sequences did not yield hits outside of the Diptera.

Figure 3.

CG4577/Spar is expressed in neuroendocrine cells

To further characterise CG4577 we generated polyclonal antibodies that are predicted to recognize both CG4577-PA and CG4577-PB and investigated protein expression. CG4577 protein was expressed in a “sparkly” pattern in neurons of the third instar central brain as well as in distinct cell bodies and neuronal processes in the ventral nerve cord, prompting us to name CG4577 as Sparkly (Spar) (Fig. 4A-B). Co-labeling of Spar and Alk confirmed expression of Spar in a subset of Alk expressing cells, in agreement with our transcriptomics analyses (Fig. 4A). In addition, we also observed expression of Spar in neuronal processes which emerge from the ventral nerve cord and appear to innervate larval body wall muscle number 8, that may be either Leukokinin (Lk) or cystine-knot glycoprotein hormone GPB5 expressing neurons (Fig. 4B) (Cantera & Nässel, 1992; Sellami et al, 2011). Spar antibody specificity was confirmed in both C155-Gal4>UAS-Spar-RNAi larvae, where RNAi mediated knock down of Spar resulted in loss of detectable signal (Fig. 4C-C’), and in C155-Gal4>UAS-Spar larvae, exhibiting ectopic Spar expression in the larval CNS and photoreceptors of the eye disc (Fig. 4D-D’). To further address Spar expression in the neuroendocrine system, we co-labelled with antibodies to Dimm to identify peptidergic neuronal soma (Allan et al., 2005) in a Dimm-Gal4>UAS-GFPcaax background. This further confirmed the expression of Spar in Dimm-positive peptidergic neuroendocrine cells in the larval CNS (Fig. 4E-E”). Moreover, co-staining of Spar and Dimm in the adult CNS showed similar results (Fig. S5).

Figure 4.

Spar expression is modulated in response to Alk signaling activity

Our initial integrated analysis predicted Spar as a locus responsive to Alk signaling. To test this hypothesis, we examined Spar protein expression in w¹¹¹⁸, Alk^Y1355S and Alk^ΔRA genetic backgrounds, in which Alk signaling output is either upregulated (Alk^Y1355S) or downregulated (Alk^ΔRA) (Pfeifer et al., 2022). We observed a significant increase in Spar protein in Alk^Y1355S CNS, while levels of Spar in Alk^ΔRA CNS appeared reduced but not significantly (Fig. 4F-H, quantified in I) (Fig. 3D). In agreement, overexpression of Jeb (C155-Gal4>jeb) significantly increased Spar levels when compared with controls (C155-Gal4>UAS-GFPcaax) (Fig. 4J-L, quantified in M). Again, while overexpression of dominant-negative Alk (C155-Gal4>UAS-Alk^DN) appeared to result in decreased Spar levels, this was not significant (Fig. 4L, quantified in M). Taken together, these observations confirm that Spar expression is responsive to Alk signaling in CNS.

Spar encodes a canonically processed neurosecretory protein

To provide biochemical evidence for the expression of Spar, we re-analysed data from a previous peptidomic analysis of flies deficient for carboxypeptidase D (dCPD, SILVER) (Pauls et al, 2019), an enzyme that removes the basic C-terminal aa of peptides originating from PC cleavage of the proprotein. The analysis identified various peptides in non-digested extracts from genetic control brains (Fig. 5), including peptides framed by dibasic prohormone cleavage sequences, one of which (SEEASAVPTAD) was also obtained by de-novo sequencing (Fig. 5). This result demonstrates that the SPAR precursor is expressed and is processed into multiple peptides by PCs and possibly also other proteases. Analysis of the brain of svr mutant flies yielded similar results, but further revealed peptides C-terminally extended by the dibasic cleavage sequence (SEEASAVPTADKK, FNDMRLKR) (Fig. 5), thereby confirming canonical PC processing of the Spar propeptide. Of note, the phylogenetically most conserved peptide sequence of the Spar precursor (DTQLNPADMLALVALVEAGERA) framed by dibasic cleavage sites was among the identified peptides yet occurred only in control but not svr mutant brains (Fig. 5).

Figure 5.

Additionally, we performed co-labeling with known Drosophila neuropeptides, Pigment-dispersing factor (PDF), Dh44, Insulin-like peptide 2 (Ilp2), AstA and Lk, observing Spar expression in subsets of all these populations (Fig. 6). These included the PDF-positive LNv clock neurons (Fig. 6A-B”), Dh44-positive neurons (Fig. 6C- D”), a subset of Ilp2 neurons in the central brain (Fig. 6E-F”) and several AstA positive neurons in the central brain and ventral nerve cord (Fig. 6G-H”). We also noted coexpression in some Lk-positive neurons in the central brain and ventral nerve cord, that include the neuronal processes converging on body wall muscle 8 (Fig. 6I- L”) (Cantera & Nässel, 1992). Similar Spar co-expression with PDF, Dh44, Ilp2, and AstA was observed in adult CNS (Fig. S6).

Figure 6.

CRISPR/Cas9 generated Spar mutants are viable

Since previous reports have shown that Jeb overexpression in the larval CNS results in a small pupal size (Gouzi et al., 2011), we measured pupal size on ectopic expression of Spar (C155-Gal4>Spar) and Spar RNAi (C155-Gal4>Spar RNAi), noting no significant difference compared to controls (C155-Gal4>+ and C155-Gal4>jeb) (Fig. S7). These results suggest that Spar may be involved in an additional Alk dependant function in the CNS. Further, experiments overexpressing Spar did not reveal any obvious phenotypes. To further investigate the function of Spar we generated a Spar loss of function allele by CRISPR/Cas9-mediated non-homologous end-joining, resulting in the deletion of a 716bp region including the Spar transcription start site and exon 1 (hereafter referred as Spar^ΔExon1) (Fig. 7A). Immunoblotting analysis indicated a 35kDa protein present in the wild-type (w¹¹¹⁸) controls that was absent in Spar^ΔExon1 mutant CNS lysates (Fig. 7B). The Spar^ΔExon1 mutant allele was further characterised using immunohistochemistry (Fig. 7C-D’). Spar^ΔExon1 shows a complete abrogation of larval and adult Spar expression, consistent with the reduction observed when Spar RNAi was employed (Fig. 7C-D’). Spar^ΔExon1 flies were viable, and no gross morphological phenotypes were observed, similar to loss of function mutants in several previously characterised neuropeptides such as Pigment dispersing factor (PDF), Drosulfakinin (Dsk) and Neuropeptide F (NPF) (Liu et al, 2019; Renn et al, 1999; Wu et al, 2020).

Figure 7.

Spar is expressed in a subset of clock-neurons in the larval and adult CNS

A previous report noted expression of Spar in the ventral lateral neuron (LNv), dorsal lateral neuron (LNd) and dorsal neuron 1 (DN1) populations of adult Drosophila circadian clock neurons (Abruzzi et al, 2017) (Fig. 7E). A meta-analysis of the publicly available single-cell transcriptomics of circadian clock neurons indicated that almost all adult cluster of clock neurons express Spar (Ma et al, 2021) (Fig. 7F). Additionally, we noted that the expression of Spar peaks around Zeitgeber time 10 (ZT10) (coinciding with the evening peak) (Fig. 7G-H), although the differences in expression level around the clock with LD or DD cycle were not dramatic (Fig. S8A-C). To confirm the expression of Spar in circadian neurons at the protein level we co-stained Spar with a clock neuron reporter (Clk856-Gal4>UAS-GFP). A subset of Spar-positive larval CNS neurons appeared to be Clk856-Gal4>UAS-GFP positive (Fig. 7I-J”). Similarly, a subset of Spar-positive neurons in adults were GFP-positive (Fig. 7K-L”), confirming the expression of Spar protein in LNv clock neurons. Taken together, these findings suggest a potential function of the Alk-regulated TaDa-identified target Spar in the maintenance of circadian rhythm in Drosophila.

Spar^ΔExon1 mutants exhibit reduced adult lifespan, activity and circadian disturbances

Given the expression of Spar in circadian neurons of the larval CNS, and the previous observations of a role of Alk mutations in sleep dysregulation in flies (Bai & Sehgal, 2015), we hypothesised that Spar^ΔExon1 mutants may exhibit activity/circadian rhythm-related phenotypes. To test this, we first investigated the effects of loss of Spar (employing Spar^ΔExon1) and loss of Alk (employing a CNS specific loss of function allele of Alk, Alk^ΔRA (Pfeifer et al., 2022)) on adult lifespan and sleep/activity behaviour using the DAM (Drosophila activity monitor) system (Trikinetics Inc.). Both Alk^ΔRA and Spar^ΔExon1 mutant flies displayed a significantly reduced lifespan when compared to w¹¹¹⁸ controls, with the Spar^ΔExon1 group exhibiting a significant reduction in survival at 25 days (Fig. 8A). Activity analysis in Alk^ΔRA and Spar^ΔExon1 flies under 12h light: 12h dark (LD) conditions indicated that both Alk^ΔRA and Spar^ΔExon1 flies exhibited two major activity peaks, the first centered around Zeitgeber time 0 (ZT0), the beginning of the light phase, the so-called morning peak, and the second around Zeitgeber time 12 (ZT12), the beginning of the dark phase that is called the evening peak (Fig. 8B, black arrows). Overall activity and sleep profiles per 24h showed increased activity in Spar^ΔExon1 flies (Fig.8B-C), that was more prominent during the light phase, with an increase in the anticipatory activity preceding both the night-day and the day-night transition in comparison to Alk^ΔRA and w¹¹¹⁸ (Fig. 8B, empty arrows). The higher locomotor activity of Alk^ΔRA and Spar^ΔExon1 flies in comparison to wild-type was also visible in individual actograms (Fig. 8D). Furthermore, the mean activity and sleep over 30 days period of the experiment were also affected; the two mutant groups (Alk^ΔRA and spar^ΔExon1) displayed significant variations in activity and sleep means (Fig. 8E-F). Chi square rhythmicity analysis showed that Alk^ΔRA and w¹¹¹⁸ in LD are more rhythmic compared to Spar^ΔExon1 flies (Fig. S10A), however when comparing percentage of rhythmic flies among all groups the differences were not significant (Fig. S9A’). Since the defects observed in behaviour in both Alk^ΔRA and Spar^ΔExon1 mutants could be age dependent, we assessed mean activity and sleep after dividing the flies into two age groups “young” (1-12 days) and “old” (12-30 days). Notably, neither wake nor sleep activity was affected in old flies but was significantly perturbed in young flies (Fig. 8G-H). These results demonstrate that Spar is important for normal fly activity and loss of spar affects adult life cycle and sleep/wake activity.

Figure 8.

Since Spar^ΔExon1 flies exhibited a hyperactive phenotype during both day and night hours, we sought to investigate a potential role of Spar in regulating the endogenous fly clock by assessing fly activity after shift to dark conditions. While control flies (Fig. 9A-A’) adapted to the light-dark shift without any effect on mean activity and sleep (Fig. 9A’, D’), Spar^ΔExon1 flies exhibited striking defects in circadian locomotor activity. Comparison of average activity and sleep during 5 days of LD (light-dark) versus 5 days of DD (dark-dark) cycles, identified a reduction in mean activity under DD conditions in Spar^ΔExon1 flies (Fig. 9B-B’), consistent with an increase in average sleep mean (Fig. 9E-E’). Actogram profiling (Fig. 9C) showed that Spar^ΔExon1 flies exhibit a hyperactive profile consistent with our previous data in LD conditions, and maintain this hyperactivity when shifted into DD conditions (Fig. 8D, S9A-B’). Upon further in-depth examination of the activity profile in Spar^ΔExon1 we noticed that the transition to DD cycle resulted in a complete loss of anticipatory peaks with no activity peaks either at CT0 or at CT12 (Fig. 9B, empty arrows) and a dramatic change in activity and sleep bouts in these mutants (Fig. 9B, E, S9) which was not seen in control flies (Fig. 9A, D). The rhythmicity strength of Spar^ΔExon1 flies was similar between LD and DD conditions (Fig. S10C), while control w¹¹¹⁸ flies were less rhythmic in DD compared to LD conditions (Fig. S10B). Both w¹¹¹⁸ and Spar^ΔExon1 flies were rhythmic (S10D-F).

Figure 9.

Discussion

With the advent of multiple omics approaches, data integration represents a powerful, yet challenging approach to identify novel components and targets of signaling pathways. The availability of various genetic tools for manipulating Alk signaling in Drosophila along with previously gathered omics dataset provides an excellent basis for Alk centered data acquisition. We complemented this with TaDa transcriptional profiling allowing us to generate a rich dataset of Alk-responsive loci with the potential to improve our mechanistic understanding of Alk signaling in the CNS. A striking observation revealed by integrating our TaDa study with scRNAseq data was the enrichment of Alk-responsive genes expressed in neuroendocrine cells. These results are consistent with previous studies reporting expression of Alk in the Drosophila larval prothoracic gland (Pan & O’Connor, 2021), the neuroendocrine functions of Alk in mice (Ahmed et al., 2022; Reshetnyak et al, 2015; Witek et al., 2015) and the role of oncogenic Alk in neuroblastoma, a childhood cancer which arises from the neuroendocrine system (Matthay et al., 2016; Umapathy et al., 2019). In this study, we focused on one target of interest downstream of Alk, however, many additional interesting candidates remain to be explored. These include CG12594, complexin (cpx) and the vesicular glutamate transporter (VGlut) that also exhibit a high ratio of co-expression with Alk in scRNAseq data (Fig. S2A).

Employing a strict context dependent filter on our integrated omics datasets identified Spar as a previously uncharacterized Alk regulated neuropeptide precursor. Spar amino acid sequence analysis predicts an N-terminal signal peptide and multiple canonical dibasic PC cleavage sites which are hallmarks of neuropeptide precursors. This is strong indication that Spar is shuttled to the secretory pathway and is post translationally processed within the Golgi or transport vesicles. Moreover, using mass spectrometry, we were able to identify predicted canonically processed peptides from the Spar precursor in undigested fly brain extracts. While all this points towards a neuropeptide-like function of Spar, other features appear rather unusual for a typical insect neuropeptide. First, the Spar propeptide is quite large for a neuropeptide precursor, and the predicted peptides do not represent paracopies of each other and do not carry a C-terminal amidation signal as is typical for Drosophila and other insect peptides (Nässel & Zandawala, 2019; Wegener & Gorbashov, 2008). Moreover, there are no obvious Spar or Spar peptide orthologues in animals outside the Diptera. We noted, however, that Spar is an acidic protein with a pI of 5.1 that lacks any cysteine residue. These features are reminiscent of vertebrate secretogranins, which are packaged and cleaved by PCs and other proteases inside dense vesicles in the regulated secretory pathway in neurosecretory cells (Helle, 2004). Secretogranins have so far not been identified in the Drosophila genome (Hart et al, 2017). Therefore, the identification of the neurosecretory protein Spar downstream of Alk in the Drosophila CNS is particularly interesting in light of previous findings, where VGF (aka secretogranin VII) has been identified as one of the strongest transcriptional targets regulated by ALK in both cell lines and mouse neuroblastoma models (Borenas et al., 2021; Cazes et al, 2014). VGF encodes a precursor polypeptide, which is processed by PCs generating an array of secreted peptide products with multiple functions that are not yet fully understood at this time (Lewis et al, 2015; Quinn et al, 2021).

Using a newly generated antibody we characterized the expression of Spar in the Drosophila CNS, showing that its expression overlaps with the Dimm transcription factor that is expressed in the fly neuroendocrine system (Hewes et al., 2003), suggesting that Spar is expressed along with multiple other neuropeptides in pro secretory cells of the CNS (Park et al., 2008). Spar is also expressed in well-established structures such as the mushroom bodies, which are known to be important in learning and memory and regulate food attraction and sleep (Joiner et al, 2006; Pitman et al, 2006), and where Alk is also known to function (Bai & Sehgal, 2015; Gouzi et al., 2011; Pfeifer et al., 2022). Interestingly, Spar is expressed in a subset of peptidergic neurons which emerge from the ventral nerve cord (VNC) and innervate larval body wall muscle number 8. In larvae these Lk-expressing neurons of the VNC, known as ABLKs, are part of circuitry that regulates locomotion and nociception, and in adults they regulate water and ion homeostasis (Imambocus et al, 2022; Okusawa et al, 2014; Zandawala et al, 2018). The role of Spar in this context is unknown and requires further investigation. The identity of the Spar receptor, as well as its location, both within the CNS and without, as suggested by the expression of Spar in neurons innervating the larval body wall is another interesting question for a future study. In our current study we focused on characterising Spar in the Drosophila CNS. To functionally characterise Spar in this context we generated null alleles with CRISPR/Cas9 and investigated the resulting viable Spar^ΔExon1 mutant.

Spar transcript expression in Drosophila clock neurons has been noted in a previous study investigating neuropeptides in clock neurons, however Spar had not been functionally characterised at the time (Abruzzi et al., 2017, Ma et al, 2021). We have been able to show that Spar protein is expressed in clock neurons of the larval and adult CNS, findings that prompted us to study the effect of Spar in activity and circadian rhythms of flies. Drosophila activity monitoring experiments with Spar^ΔExon1 and Alk loss of function (Alk^ΔRA) mutants revealed striking phenotypes in life span, activity and sleep. In Drosophila a number of genes and neural circuits involved in the regulation of sleep have been identified (Shafer & Keene, 2021). The role of Alk in sleep has previously been described in the fly, where Alk and the Ras GTPase Neurofibromin1 (Nf1), function together to regulate sleep (Bai and Sehgal, 2015). Indeed, a study in mice has reported an evolutionarily conserved role for Alk and Nf1 in circadian function (Weiss et al, 2017). While these studies place Alk and Nf1 together in a signaling pathway that regulates sleep and circadian rhythms, no downstream effectors transcriptionally regulated by the Alk pathway have been identified that could explain its regulation of Drosophila sleep/activity. Our data suggest that one way in which Alk signaling regulates sleep is through the control of Spar, as Spar^ΔExon1 mutants exhibit a striking activity phenotype. The role of clock neurons and the involvement of circadian input in maintenance of long term memory (LTM) involving neuropeptides such as PDF has been previously described (Inami et al, 2022). Since both Alk and Nf1 are also implicated in LTM formation in mushroom body neurons (Gouzi, Bouraimi et al 2018), the potential role of Nf1 in Spar regulation and the effect of Spar loss on LTM will be interesting to test in future work. It can be noted that both insulin producing cells (IPCs) and DH44 cells of the pars intercerebralis and the Lk producing LHLK neurons of the brain and certain AstA neurons in the brain are involved in regulation of aspects of metabolism and sleep (Barber et al, 2021; Cavey et al, 2016; Chen et al, 2016; Cong et al, 2015; Donlea et al, 2018; Nässel & Zandawala, 2022; Yurgel et al, 2019). Furthermore, DH44 cells of pars intercerebralis in S Fig 6 C,D are major players in regulation feeding and courtship in adults (Barber et al., 2021; Cavanaugh et al, 2014; Dus et al, 2015; King et al, 2017; Oh et al, 2021). In conclusion, our TaDa analysis identifies a role for Alk in regulation of endocrine function in Drosophila. These results agree with the previously reported broad role of Alk in functions such as sleep, metabolism, and olfaction in the fly and in the hypothalamic-pituitary-gonadal axis and Alk-driven neuroblastoma responses in mice. Finally, we identify Spar as the first neuropeptide precursor downstream of Alk to be described that regulates activity and circadian clock function in the fly.

Materials and methods

Drosophila stocks and Genetics

Standard Drosophila husbandry procedures were followed. Flies were fed on Nutri Fly® Bloomington Formulation food (Genesee Scientific, Inc.) cooked according to the manufacturer’s instruction. Crosses were reared at 25°C. The following stocks were obtained from Bloomington Drosophila Stock Center (BDSC): w¹¹¹⁸ (BL3605), Dimm- Gal4 (also known as C929-Gal4) (BL25373), and C155-Gal4 (BL458). Additional stocks used in this study are the following: UAS-LT3-NDam-Pol II (Southall et al., 2013), UAS-Alk^DN (P{UAS-Alk.EC.MYC} (Bazigou et al., 2007)), UAS-Jeb (Varshney & Palmer, 2006), Alk^Y1335S (Pfeifer et al., 2022), Alk^ΔRA (Pfeifer et al., 2022), Spar^ΔExon1 (this study), UAS-Spar (this study) and UAS-Spar^2xOLLAS (this study).

TaDa Sample preparation

Pan neuronal C155-Gal4 expressing animals were crossed with either UAS-LT3 Dam::Pol II (Control) or UAS-LT3-Dam::Pol II; UAS-Alk^EC (Alk dominant negative sample) and crosses were reared at 25°C. Approximately 100-150 third instar larval brains were dissected in cold PBS for each technical replicate. Genomic DNA was extracted using QIAGEN blood and tissue DNA extraction kit and methylated DNA was processed and amplified using previously described (Choksi et al, 2006; Sun et al, 2003) with the following modifications. After genomic DNA extraction, non-sheared gDNA was verified on 1.5% agarose gel, and an overnight DpnI digestion reaction setup in a 50 µl reaction volume. The digestion reaction was subsequently purified using QIAGEN MiniElute PCR purified Kit and eluted in 50 µl MQ water. 50 µl of DpnI digested and purified DNA was further used for adaptor ligation. Adaptor ligated DNA was amplified using the adaptor specific primer to generate the TaDa-seq library. Amplified DNA from all experimental conditions was repurified (QIAGEN MiniElute PCR purification kit) into 20 µl of MQ water and 200 ng aliquots were run on 1% agarose gel to verify amplification of TaDa library (DNA fragments ranging from 500bp to 3Kb). The TaDa library was used for PCR-free library preparation followed by paired-end sequencing on a Illumina HiSeq 10x platform (BGI Tech Solutions, Hong Kong).

TaDa bioinformatics data analysis

Paired TaDa FASTQ sequences of the control sample with three biological replicates and dominant negative samples with two biological replicates (with two technical replicates for both control and dominant negative samples) were obtained for a total of 10 samples and used for subseqent analysis. After base quality assessment, reads were mapped to the reference genome of Drosophila melanogaster using Bowtie2 (-- very-sensitive-local) (Langmead & Salzberg, 2012) and post alignment processes were performed with sam tools and BED tools (Barnett et al, 2011; Quinlan, 2014). The Drosophila melanogaster reference sequence (FASTA) and gene annotation files were downloaded from Flybase and all GATC coordinates were extracted using fuzznuc (Rice et al, 2000) in BED format. Replicates were merged using Sambamba (merge) (Tarasov et al, 2015), and log-fold changes between control and dominant negative, obtained by deeptools bamCompare (--centerReads --scaleFactorsMethod readCount --effectiveGenomeSize 142573017 --smoothLength 5 -bs 1) (Ramirez et al, 2014) for BIGWIG (BW) file generation. Counts of reads mapped to GATC border fragments were generated using a perl script (GATC_mapper.pl) from DamID-Seq pipeline (Maksimov et al, 2016). GATC level counts were converted to gene level counts using Bedtools (intersectBed) (Quinlan, 2014). GATC sites were merged into peaks based on a previous study (Tosti et al., 2018). LogFC for individual GATC sites were generated using Limma for dominant negative vs control (P < 1e-5) and GATC sites were merged into peaks based on median GATC fragment size in the Drosophila genome assembly using mergeWindows (tol=195, max.width=5000) and combineTests function from the csaw package (Lun & Smyth, 2016). Peaks were assigned to overlapping genes and filtered for FDR smaller than 0.05 and the mean logFC less than -2. All peak calling and statistical analysis was performed using the R programming environment. TaDa data can also be visualized using a custom UCSC (University of California, Santa Cruz) Genome Browser session (https://genome-euro.ucsc.edu/s/vimalajeno/dm6). WebGestaltR (Liao et al, 2019) was used for GO (Gene Ontology) for significantly downregulated TaDa candidates.

Integration of TaDa data with scRNA-seq and other omics data

Previously published wild-type third instar larval brain scRNA-Seq data (GSE198850) was employed (Pfeifer et al., 2022). Cellular heterogeneity was determined with eight different types of cells, including immature neurons, mature neurons, early neuroblast, NB-enriched cells, NB proliferating cells, optic lobe epithelium (OLE), Repo-positive cells and Wrapper-positive cells. The mature neuron population was divided into two groups for the current study: mature neurons and neuroendocrine cells. The neuroendocrine cell cluster was determined based on canonical markers (Guo et al., 2019; Huckesfeld et al., 2021; Nässel, 2018; Takeda & Suzuki, 2022; Torii, 2009). Subsequent analysis, including dimensionality reduction/projection or cluster visualization and marker identification was performed using R (Seurat) (Stuart et al, 2019) and Python (Scanpy) (Wolf et al, 2018) packages. Marker genes for each cluster were identified by FindAllMarkers function (Seurat) (Stuart et al., 2019). Clusters were visualized using two-dimensional Uniform Manifold Approximation and Projection (UMAP). The top 500 significantly downregulated genes from TaDa data (FDR<0.05 and mean logFC≤-2) were analysed in the third instar larval brain scRNAseq data. These 500 candidates were used as gene signatures, and enrichment analysis carried out using AUCell to determine whether a subset of the input gene set was enriched for each cell (with an enrichment threshold set at >0.196), and the clusters projected in UMAP based on the signature score (AUC score) (Aibar et al., 2017). Violin plots, dot plots, feature plots, heatmaps and matrix plots were plotted to visualize gene expression in the scRNAseq data. Functional enrichment analysis for the common significantly downregulated genes from the TaDa analysis was compared to neuroendocrine cell markers using WebGestaltR (Liao et al., 2019).

Circadian neuron scRNA-Seq data analysis

Publicly available circadian neuron scRNA-Seq data (10x) from the GEO database (GSE157504) was employed to investigate expression of CG4577 in circadian neurons (Ma et al., 2021). The dataset d includes two conditions: LD (Light and Dark) and DD (Dark and Dark), as well as six time points: 2 hours, 6 hours, 10 hours, 14 hours, and 22 hours. After preprocessing, 3172 and 4269 cells remained for the LD and DD samples respectively, with a total of 15,743 and 15,461 RNA features. Subsequent analysis, including integration, dimensionality reduction/projection and cluster visualization was performed using R (Seurat) (Stuart et al., 2019). Based on clustering, 17 clusters were defined and visualized using two-dimensional Uniform Manifold Approximation and Projection (UMAP). Violin plots, dot plots, and feature plots were employed to visualize gene expression.

Immunohistochemistry

Relevant tissue (larval CNS or body wall muscle preparation) was dissected in cold PBS and tissues fixed in 4% formaldehyde at 4°C for 1 hour. Samples were washed 3 times with 0.1% PBS Triton X-100, followed by overnight incubation in 4% goat serum, 0.1% PBS Triton X-100. The following primary antibodies were used: guinea pig anti-Alk (1:1000, (Loren et al., 2003)), rabbit anti-Alk (1:1000, (Loren et al., 2003)), and rabbit anti-Dimm (1:1000 (Allan et al, 2005)), mouse mAb anti-PDF (1:1000, DSHB: C7), rabbit anti-Ilp2 (1:1000, (Veenstra et al, 2008)), anti-Dh44 (1:1000, (Cabrero et al, 2002)), rabbit anti-AstA (1:3000, (Stay et al, 1992) (Vitzthum et al, 1996), Jena Bioscience GmbH), rabbit anti-Lk (1:1000, (Cantera & Nässel, 1992)), guinea pig anti-Spar (1:2000, this study), and Alexa Fluor®-conjugated secondary antibodies were from Jackson Immuno Research. Corrected total cell fluorescence (CTCF) was calculated using FIJI (Schindelin et al, 2012) by determining the level of cellular fluorescence and fluorescence intensity from microscopy images in third instar larval brains (n≥3 per genotype).

Immunoblotting

Third instar larval brains were dissected and lysed in cell lysis buffer (50 mM Tris-Cl, pH7.4, 250 mM NaCl, 1 mM EDTA, 1 mM EGTA, 0,5% Triton X-100, complete protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail) on ice for 20 minutes prior to clarification by centrifugation at 14,000 rpm at 4°C for 15 minutes. Protein samples were then subjected to SDS-PAGE and immunoblotting analysis. Primary antibodies used were: guinea pig anti-Spar (1:1000) (this study) and anti tubulin (Cell Signaling #2125, 1:20,000). Secondary Antibodies used were: Peroxidase Affinipure Donkey Anti-Guinea Pig IgG (Jackson ImmunoResearch #706-035-148) and goat anti-rabbit IgG (Thermo Fisher Scientific # 32260, 1:5000).

Generation of anti-Spar antibodies

Polyclonal antibodies against Spar (CG4577) were custom generated in guinea pigs by Eurogentec. Two Spar peptides corresponding to epitopes LQEIDDYVPERRVSS (amino acids 212-226) and PVAERGSGYNGEKYF (amino acids 432-446) of Spar-PA were injected simultaneously.

Biochemical identification of Spar peptides

We re-examined peptidomic data from our previous study on the role of Drosophila carboxypeptidase D (SILVER) in neuropeptide processing (Pauls et al., 2019) for the occurrence of Spar. Peptides were extracted from brains from 5 d old male flies and analyzed on an Orbitrap Fusion mass spectrometer (Thermo Scientific) equipped with a PicoView ion source (New Objective) and coupled to an EASY-nLC 1000 system (Thermo Scientific) (for details see (Pauls et al., 2019)). Database search was performed against the UniProt Drosophila melanogaster database (UP000000803; 22070 protein entries) with PEAKS XPro 10.6 software (Bioinformatics solution) with the following parameters: peptide mass tolerance: 8 ppm, MS/MS mass tolerance: 0.02 Da, enzyme: “none”; variable modifications: Oxidation (M), Carbamidomethylation (C), Pyro-glu from Q, Amidation (peptide C-term). Results were filtered to 1% PSM-FDR.

CRISPR/Cas9 mediated generation of the Spar^ΔExon1 mutant

The Spar^ΔExon1 mutant was generated using CRISPR/Cas9 genome editing. Design and evaluation of CRISPR target sites was performed using the flyCRISPR Optimal Target Finder tool (Gratz et al., 2015). Single guide RNA (sgRNA) targeting sequences (sequences available in Table S1) were cloned into pU6-BbsI-chiRNA vector 2 (Addgene, Cat. No. 45946) and injected into vasa-Cas9 (BDSC, #51323) embryos (BestGene Inc.). Injected flies were crossed to second chromosome balancer flies (BDSC, #9120) and their progeny were PCR-screened for progenitors carrying a 716 bp deletion event. Mutant candidates were confirmed by Sanger sequencing (Eurofins Genomics).

Generation of UAS-Spar fly lines

UAS-Spar was generated by cloning (GeneScript) the coding sequence of CG4577-RA into EcoRI/XbaI-cut pUASTattB vector followed by injection into fly embryos (BestGene Inc.) using attP1 (2^nd chromosome, BDSC#8621) and attP2 (3^rd chromosome, BDSC#8622) docking sites for phiC31 integrase-mediated transformation. Injected flies were crossed to second or third chromosome balancer flies, and transgenic progeny identified based on the presence of mini-white marker.

Measurement of Pupal size

Late pupae of the indicated genotype were collected and placed on glass slides with double-sided tape. Puparuim were imaged under Zeiss Axio Zoom.V16 stereo zoom microscope with a light-emitting diode ring light and measured using Zen Blue edition software. Both female and male pupae, picked randomly, were used for measurements.

Drosophila activity monitor assay

Up to 32 newly eclosed male flies were transferred into individual glass tubes containing food media (1% agar and 5% sucrose), which were each placed into a DAM2 Drosophila activity monitor (Trikinetics Inc). Monitors were then placed in a 25°C incubator running a 12:12h light:dark cycle, at a constant 60% humidity. Activity was detected by an infrared light beam emitted by the monitor across the center of each glass tube. The experiment was carried out for one month, and the raw binary data was acquired by the DAMSystem310 software (Trikinetics Inc.). The LD/DD experiment was performed according to previously published work (Chiu et al, 2010); adult flies were first entrained for 5 days in normal light:dark cycle and on the last day (LD5), the light parameters were switched off and flies were then conditioned in complete dark:dark settings for 7 days. Statistical and data analyses were carried out using Microsoft Office and GraphPad Prism 8.4.2, taking into consideration 5 min of inactivity as sleep and more than 24h of immobility as a death event. Actogram activity profile charts were generated using ActogramJ 1.0 (https://bene51.github.io/ActogramJ/index.html) and ImageJ software (https://imagej.nih.gov/ij/). ActogramJ was further used to generate the chi-square periodogram for each single fly in order to calculate the power value of rhythmicity and the percentage of rhythmic flies.

Data visualization and schematics

Schematics were generated at Biorender.com and Bioicons.com. The pipeline icon by Simon Dürr https://twitter.com/simonduerr is licensed under CC0 https://creativecommons.org/publicdomain/zero/1.0/ . Boxplots in Figure 3D and Suppl. Figure 7 were generated using BoxplotR (http://shiny.chemgrid.org/boxplotr/). Boxplots in Figure 4 were generated using GraphPad Prism 9.

Acknowledgements

The authors thank Jonathan Benito Sipos and Stefan Thor for the kind gift of anti Dimmed antibodies, as well as Jan Veenstra for kindly gifting anti-Dh44 and anti-Ilp2. C7 anti-PDF (developed by J. Blau) was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242. We acknowledge Bloomington Drosophila Stock Center (NIH P40OD018537) for fly stocks used in this study. We thank Hisae Mori for providing support for fly lab maintenance. We thank members of the Palmer, Hallberg lab and Anne Uv for critical feedback on the manuscript. This work has been supported by grants from the Swedish Cancer Society (RHP CAN18/0729), the Children’s Cancer Foundation (RHP 2019-0078), the Swedish Research Council (RHP 2019-03914), the Swedish Foundation for Strategic Research (RB13-0204), the Göran Gustafsson Foundation (RHP2016) and the Knut and Alice Wallenberg Foundation (KAW 2015.0144). MS and JS are supported by the Medical Practice Plan (MPP) at the American University of Beirut.

Author contributions

S.K.S. and R.H.P. conceived the research. Wet lab experiments were conducted by S.K.S., L.M., J.S., G.U., P.M.-G., and T.M.. V.A. carried out all bioinformatics analysis. L.M. assisted with Spar mutant generation, validation and immunohistochemistry. J.S. performed and analyzed all Drosophila activity monitoring experiments under the supervision of M.S.. G.U. performed immunoblotting. P.M.-G. assisted in optimizing and performing TaDa experiments and T.M. performed image analysis and assisted with immunohistochemistry. A.S. and C.W. analyzed mass spectrometry peptidomic data. D.N. provided critical feedback and design input. R.H.P and M.S. supervised the project. S.K.S. and R.H.P. wrote the first manuscript draft that was further developed with all authors.

Competing interests

The authors declare that they have no competing interests

Data Availability Statement

The original contributions presented in the study are included in the article/Supplementary Material. The TaDa dataset has been deposited in Gene Expression Omnibus (GEO) under the accession number GSE229518. The genome browser tracks for the TaDa peak analysis can be found at: https://genome-euro.ucsc.edu/s/vimalajeno/dm6.

Supplementary figure legends

Supplementary Figure 1. TaDa third instar larval CNS sample validation and additional data analysis. A-B’. Expression of mCherry in the larval CNS reflects Dam-PolII expression. Third instar larval brains were stained for Alk (in green) and mCherry (in magenta) confirming expression of Dam-PolII in the TaDa system. Scale bars: 100 μm. C. Schematic overview of the TaDa analysis experimental workflow. Brains from third instar wandering larvae were dissected, and methylated DNA digested with Dpn1 restriction endonuclease. The resulting DNA fragment library was amplified, sequenced and analysed through TaDa bioinformatics pipelines. D. Bar graph showing total number of reads in each replicate of the TaDa dataset. D’. Bar graph showing percentage of reads aligned to Drosophila genome in each replicate. E. Correlation plot of samples (control and Alk^DN) and replicates shows no significant intra-replicate differences. F. Line graph indicating the relative distance to TSS of different samples compared to random regions. G. Pol ll occupancy profile of Alk in Alk^DN compared to control indicates a higher pol II occupancy in exons 1 to exon 7, in agreement with the expression of the Alk^DN transgene.

Supplementary Figure 2. Feature plots visualizing expression of TaDa-identified genes expressed in neuroendocrine cells in scRNA-seq from third instar larval CNS (Pfeifer et al., 2022). TaDa candidates CG12594, cpx and VGlut are shown.

Supplementary Figure 3. Co-expression of Alk and Spar in publicly available Drosophila CNS scRNA-seq datasets. UMAP showing co-expression of Alk and CG4577 in different cell clusters in publicly available scRNA-seq data (Brunet Avalos et al., 2019) from first instar larval CNS (A) and (B) adult CNS (Davie et al., 2018). C. Pairwise alignment of CG4577-PA and CG4577-PB showing isoform-specific differences in amino acid positions 405 and 406 (highlighted in yellow).

Supplementary Figure 4. Alignment of CG4577 orthologs in flies (Brachycera). A. Alignment of Drosophila melanogaster CG4577 orthologs in the family Drosophilidae (vinegar flies, including the fruit fly Drosophila melanogaster). B. Aligment of Drosophila melanogaster CG4577 orthologs in other brachyceran taxa.

Supplementary Figure 5. A-A” Adult CNS showing Spar expression in Dimm positive cells. Spar (in magenta) and Dimm (in green), close-ups (B-B”) indicated by boxed regions and arrows indicating representative co-expressed markers cells. Scale bars: 100 μm.

Supplementary Figure 6. Spar expression in adult neuropeptide expressing neuronal populations. A. Immunostaining of w¹¹¹⁸ third adult CNS with anti-Spar (in magenta) and anti-PDF (in green). Closeups (B-B”) of PDF- ans Spar-positive LNv neurons, indicated by white arrowheads. C. Immunostaining of w¹¹¹⁸ adult CNS with Spar (in magenta) and Dh44 (in green). Closeups (D-D”) of Dh44- and Spar-positiv e neurons, indicated by white arrowheads. E. Immunostaining of w¹¹¹⁸ adult CNS with Spar (in magenta) and Ilp2 (in green). Closeups (F-F”) showing the close proximity of Ilp2-positive and Spar-positive neurons in central brain, indicated by white arrowheads. G. Immunostaining of w¹¹¹⁸ adult CNS with Spar (in magenta) and AstA (in green). Closeups (H-H”) showing AstA- and Spar-positive neurons in central brain indicated by white arrowheads. Scale bars: 100μm.

Supplementary Figure 7. Spar does not affect the Alk-regulated pupal size phenotype. Overexpression of Spar (C155-Gal4>UAS-Spar) or Spar RNAi (C155-Gal4>UAS-Spar RNAi) in CNS does not significantly affect pupal size compared to previously characterized controls such as Alk^DN (C155-Gal4>UAS-Alk^DN), which significantly increase pupal size and overexpression of Jeb (C155-Gal4>UAS-Alk^DN), which significantly decrease pupal size compared to controls (C155-Gal4>+) (n.s = not significant, **p<0.05, ***p<0.01).

Supplementary Figure 8. Spar expression in circadian neuronal clusters. A-B. Feature plots depicting the expression of Spar in publicly available circadian neuronal scRNA-seq data (Ma et al., 2021) throughout the LD cycle (Zeitgeber time) (A) and DD cycle (Circadian time) (B). C. Dotplot showing Spar expression throughout the DD cycle along with the previously characterized circadian associated neuropeptide Pdf and the core clock gene Per. Peak expression of Spar and Per is observed at CT10.

Supplementary Figure 9. A. Representative activity profile graph of w¹¹¹⁸ and Spar^ΔExon1 illustrating the average activity count measured every 5 min across a 24 hour span obtained by averaging 5 days in light/dark conditions (LD1-LD5). An unpaired student t-test was used to determine the significance between the two groups (****p<0.0001). A’. Graph illustrating the mean locomotor activity per day of w¹¹¹⁸ and Spar^ΔExon1 obtained by averaging 5 days in light/dark conditions (LD1-LD5). An unpaired student t-test was used to determine the significance between the two groups (****p<0.0001). B. Representative activity profile graph of w¹¹¹⁸ and Spar^ΔExon1 illustrating the average activity count measured every 5 min across a 24-hour span obtained by averaging 5 days in dark/dark conditions (DD1-DD5). CT0 and CT12 represent the start and end of the constant dark conditions respectively. An unpaired student t-test was used to determine the significance between the two groups (****p<0.0001). B’. Graph illustrating the mean locomotor activity per day of w¹¹¹⁸ and Spar^ΔExon1 obtained by averaging 5 days in dark/dark conditions (DD1-DD5). An unpaired student t-test was used to determine the significance between the two groups (****p<0.0001). C. Representative sleep profile graph of w¹¹¹⁸ and Spar^ΔExon1 illustrating the average activity count measured every 5 min across a 24-hour span obtained by averaging 5 days in light/dark conditions (LD1-LD5). An unpaired student t-test was used to determine the significance between the two groups (****p<0.0001). E’. Graph illustrating the mean sleep per day of w^{1118 and} Spar^ΔExon1 obtained by averaging 5 days in light/dark conditions (LD1-LD5). An unpaired student t-test was used to determine the significance between the two groups (****p<0.0001). F. Representative sleep profile graph of w¹¹¹⁸ and Spar^ΔExon1 illustrating the average activity count measured every 5 min across a 24-hour span obtained by averaging 5 days in dark/dark conditions (DD1-DD5). An unpaired student t-test was used to determine the significance between the two groups (****p<0.0001). F’. Graph illustrating the mean sleep per day of w¹¹¹⁸ and Spar^ΔExon1 obtained by averaging 5 days in dark/dark conditions (DD1-DD5). An unpaired student t-test was used to determine the significance between the two groups (****p<0.0001).

Supplementary Figure 10. Spar^ΔExon1 flies retain a hyperactive profile when shifted to dark/dark conditions. A. Graph illustrating the Qp statistical value (rhythmicity power) obtained by generating the Chi-square periodograms of w¹¹¹⁸, Spar^ΔExon1, and Alk^ΔRA flies. An unpaired student t-test was used to determine the significance between wild-type and each mutant group (****p<0.0001). A’. Representative graph of the percentage of rhythmicity of w¹¹¹⁸, Spar^ΔExon1, and Alk^ΔRA flies. B. Graph illustrating the Qp statistical value obtained by generating the Chi square periodograms of w¹¹¹⁸ flies in 5 days LD and 7 days DD conditions. A paired student t-test was used to determine the significance between the two experimental conditions (***p<0.001). C. Graph illustrating the Qp statistical value obtained by generating the Chi-square periodograms of Spar^ΔExon1 flies in 5 days LD and 7 days DD conditions. A paired student t-test was used to determine the significance between the two experimental conditions. D. Representative graph of the percentage of rhythmicity of w¹¹¹⁸ flies in LD vs DD conditions. E. Representative graph of the percentage of rhythmicity of Spar^ΔExon1 flies in LD vs DD conditions.