Angiogenesis is a morphogenic process resulting in the formation of new blood vessels from pre-existing ones, usually in hypoxic micro-environments. The initial steps of angiogenesis depend on robust differentiation of oligopotent endothelial cells into the Tip and Stalk phenotypic cell fates, controlled by NOTCH-dependent cell-cell communication. The dynamics of spatial patterning of this cell fate specification are only partially understood. Here, by combining a controlled experimental angiogenesis model with mathematical and computational analyses, we find that the regular spatial Tip-Stalk cell patterning can undergo an order-disorder transition at a relatively high input level of a pro-angiogenic factor VEGF. The resulting differentiation is robust but temporally unstable for most cells, with only a subset of presumptive Tip cells leading sprout extensions. We further find that sprouts form in a manner maximizing their mutual distance, consistent with a Turing-like model that may depend on local enrichment and depletion of fibronectin. Together, our data suggest that NOTCH signaling mediates a robust way of cell differentiation enabling but not instructing subsequent steps in angiogenic morphogenesis, which may require additional cues and self-organization mechanisms. This analysis can assist in further understating of cell plasticity underlying angiogenesis and other complex morphogenic processes.
We investigate the spatial and temporal patterns of Tip/Stalk specification and the ensuing angiogenic sprouting by using a novel controlled micro-engineered experimental model of angiogenesis and a set of mathematical models of the spatially resolved, cell population-level VEGF-NOTCH signaling. Our analysis provides a dynamic view of the initial step of angiogenesis, revealing fluctuations in its onset, and features suggesting transitions between order and disorder in cell organization. These findings suggest how a potentially very restrictive patterning mechanism can become sensitive to a variety of environmental cues. This sensitivity can be crucial for proper vascularization of a damaged organ, and may suggest new ways of analyzing angiogenesis in the context of cancer and other pathologies. This analysis also suggests a framework for understanding of other instances of NOTCH-mediated patterning processes.
This study provides important, detailed insights into the early stages of angiogenesis and points towards a novel mechanism that could be used to control the density and location of developing blood vessels. Convincing experimental methods, analysis techniques, and mathematical modeling are used to systematically support the findings. This study of the mechanisms by which paracrine and juxtacrine cues control the formation of new blood vessels will be of broad interest to colleagues studying angiogenesis and blood vessel formation.
Angiogenesis, i.e., the formation of new blood vessels from the pre-existing ones, is a striking example of phenotypic plasticity in an adult differentiated endothelium. Pro-angiogenic factors secreted in response to hypoxic conditions, particularly the vascular endothelial growth factor (VEGF), specify differentiation of endothelial cells lining blood vessels into diverse phenotypic states, including the pro-migratory Tip cell phenotype. Tip cells can initiate invasive cell migration into the surrounding extracellular matrix (ECM), leading to sprouting and branching of the nascent vessels(1, 2). Tip cells are differentiated from Stalk cells, another phenotypic state, through juxtacrine cell-cell interaction between these cell types involving NOTCH1 signaling, triggered and modulated by induction of Dll4 and Jag1 ligands(3, 4). Stalk cells can therefore form in immediate proximity of Tip cells, particularly, at the leading edge of an extending sprout, if the NOTCH signaling is sufficiently pronounced for the Tip-Stalk differentiation to occur. Proliferation of Stalk cells is as essential as the invasive migration of Tip cells for the emergence, extension and branching of growing sprouts, making the analysis of coordinated Tip and Stalk specification particularly important.
The inputs specifying the cell fate can be potentially contradictory, e.g., with pro-angiogenic factors, such as VEGF, promoting the Tip cell fate, and the NOTCH signaling activated by the neighboring cells promoting the Stalk cell fate and thus suppressing the Tip cell identity in the same cell. These and other signaling inputs can thus be incoherent in terms of cell fate specification and can result in complex dynamic outcomes that are still poorly understood. Further elucidation of these processes thus requires high resolution, quantitative experimental measurements tightly coupled with computational analysis. Since such measurements are still challenging in vivo, particularly in mammalian tissues, use of tissue models recapitulating the salient features of the developing vasculature is a key tool in the current analysis of angiogenesis and development of de novo vascular beds.
Previously, we and others have developed a set of micro-fabricated experimental angiogenesis models that have had progressively improved biomimetic characteristics(5-9). These characteristics include spatially and biochemically appropriate cell micro-environments, composed of components of the extracellular matrix and of gradients of growth factors and cytokines around the developing vasculature, which is composed of endothelial cells and pericytes.
We have previously used this approach to map different combinations of VEGF and an inflammatory cytokine, TNF, onto pro- and anti-angiogenic outcomes, modeling frequently encountered angiogenesis conditions(7). This analysis provided evidence that ‘mini-sprouts’ — one-cell structures protruding from the parental blood vessel into the surrounding matrix — were comprised of Tip cells. However, it was not clear whether all such ‘mini-sprouts’ would ultimately develop into more mature multi-cellular sprouts with defined lumens and the potential to form new blood vessels. Furthermore, although our analysis was successful in explaining the fraction of Tip cells formed under different conditions, it was not clear how to account for the spatial aspects of the Tip cell and mature sprout specification, such as their mutual separation and density.
We address these challenges here by extending our analysis to a higher temporal and spatial resolution, both in experimental and mathematical models of angiogenic sprouting. Surprisingly, we found that the formation of mini-sprouts was a highly dynamic process, in which they could either retract after extension or form fullfledged sprouts. Furthermore, the experimentally determined spatial positioning of mini-sprouts was well explained by the predicted locations of the Tip cells in the mathematical model but the model could not account for which of the mini-sprouts would become fully formed spouts. Further analysis revealed that the stable sprout formation from mini-sprouts can be enabled by the local fluctuations of the density of fibronectin, a key pro-angiogenic ECM component, leading to sparse patterns where sprouts tend to maximally distance themselves from other fully formed sprouts. These results reveal some of the key mechanisms that may define the density of the angiogenically formed vascular beds under diverse conditions.
Dynamic angiogenesis can be explored in a 3D biomimetic experimental setup
To investigate the properties of angiogenic patterning and cell fate specification, we used an experimental model previously employed to assess the crosstalk between pro-angiogenic and pro-inflammatory stimuli(7). In this experimental setup, angiogenesis occurs from a 3D parental engineered endothelial vessel embedded in the collagen matrix and exposed to exogenously supplied VEGF and other pro-angiogenic factors (Fig. 1A-B). In agreement with prior observations, we found that this setup resulted in formation of both one-cell extensions into the matrix (mini-sprouts) and full-fledged multi-cellular sprouts containing detectable lumens and pronounced leading Tip cells (Fig. 1C). Sprouts displayed a variety of growth stages, including the very early ones, composed of one lumenized cell or pairs of connected cells, also forming a lumen (Table 1). Although mini-sprouts formed throughout the observation area of the vessel, sprouts developed within specific zones, while other zones remained devoid of detectable spout formation over the course of the study (Figs. 1D-F). These observations suggested that cell fate specification and sprout formation are dynamic processes that may display diverse local outcomes. We therefore set out to characterize these processes in the context of an accessible and well defined analysis tool that can allow to contrast experimental findings with mathematical models of angiogenic patterning, particularly those based on the commonly assumed NOTCH receptor mediated cell-cell interactions (Fig. 1G).
Mathematical model of VEGF/NOTCH signaling predicts spatially resolved Tip-Stalk patterns
To set the framework for the analysis of cell fate determination, we extended our previously developed and experimentally validated mathematical model of Tip-Stalk fate differentiation between two cells(10) to a multicellular hexagonal lattice in two-dimensions. In the new model, we replicated within each cell the signaling network incorporating the NOTCH and VEGF pathways (Fig. 2A). Prior analysis of this model on the level of two adjacent cells predicted the emergence of bistability between a (high NOTCH, low Delta) Stalk phenotype and a (low NOTCH, high Delta) Tip phenotype(10). This result is consistent with the overall expectation of the differentiation effect of Delta-NOTCH signaling, which in 2D is further expected to generate ‘salt-and-pepper’ patterns, with a single Tip cell surrounded by 6 Stalk cells (11), yielding the overall fraction of Tip cells in this arrangement of 25%. However, this simple bistability and spatial patterning picture can be altered by signaling inputs that potentially conflict with those involved in Delta-NOTCH signaling (Fig.1G). For example, the VEGF pro-angiogenic factor promoting the Tip cell fate, can conflict with the NOTCH signaling activated by the neighboring cells that instead promotes the Stalk cell fate while suppressing the Tip cell identity. This might result in disordered patterns with adjacent Tip cells that deviate from the archetypical salt-and-pepper configuration. To explore the properties of this disordering effect, we ran simulations, in which the VEGF-NOTCH signaling occurred in all individual cells within a hexagonal array of the model multi-cellular endothelium, starting from randomized initial conditions (Fig. 2A). The fully equilibrated patterns were then analyzed for distributions of the simulated Delta and NOTCH expression across the cells. We found for a wide range of VEGF inputs that the distributions of Delta and NOTCH displayed largely bimodal distributions, and the levels of the average Delta expression increased with the increasing input (Fig. 2B-C, supplementary Fig. 1A-B) due to positive effect of the activated VEGF receptor on Delta (see again the circuit in Fig. 2A). Nevertheless, the clear overall bimodality allowed us to consistently classify cells into the Delta-high (‘Tip’) and Delta-low (‘Stalk’) cell states and examine the spatial distribution of these cellular sub-types (see Method section: Mathematical model of VEGF/NOTCH signaling).
The spatial Tip-Stalk cell distribution patterns revealed a complex dependency on the VEGF input. At relatively low VEGF levels, the patterns were mostly ordered, with small deviations from the expected ‘salt’ and paper geometry with a 25%-75% ratio of tip-stalk (Fig. 2D). However, as the VEGF input increased, the fraction of tips grew and the patterns became sharply more disordered over a relatively narrow range of magnitude of the VEGF input, and then became progressively further disordered, but with a lower VEGF sensitivity, over several more orders of magnitude of VEGF inputs (Fig. 2D-E and supplementary Fig. 1A-B). Spatial patterns in the disordered phase at high VEGF input levels were characterized by much higher fractions of Tip cells that were frequently in contact with each other as quantified by a “disorder index” (supplementary Fig. 1C-D). This transition was reminiscent of the order-disorder transition commonly observed and studied in the change of the order of atoms in various substances as a function of temperature, and in other chemical systems (12, 13). As expected for these types of transitions, increasing or decreasing the order-stabilizing Delta-NOTCH cell-cell signaling, resulted in the corresponding shifts of the ranges of VEGF inputs, over which the sharp order-disorder transition occurred. For example, a small increment or decrease in the cellular production of the Delta ligand shifted the order-disorder transition to either lower or higher VEGF inputs (Fig. 2F). As the broad sweep of the VEGF inputs simulated in the mathematical model is likely beyond the range of receptor sensitivity for the experimental VEGF signaling, we contrasted the predicted fractions of Tip cells with the previously made observations in the 3D experimental angiogenesis model shown in Fig. 1. We found that, for the VEGF = 10 ng/ml, the experimentally determined fraction of the Tip cells was 0.20 ± 0.08 (mean± SD), i.e., encompassing the fraction of 0.25 expected for the completely ordered, ‘salt-and-pepper’ Tip cell distribution pattern. However, for VEGF = 100 ng/ml the experimentally observed Tip cell fraction was 0.32 ± 0.01 (mean ±SD), which corresponded to the disordered state predicted by the model under higher VEGF inputs. We therefore concluded that the transition between order and disorder can occur in the 10-100 ng/ml range of VEGF concentration, allowing us to investigate the properties of the disordered state and its relationship to the spatial frequency of sprout formation. Based on the Tip/Stalk cell ratio, we calibrated the model’s parameters so that a VEGF input of 100 ng/ml matched the experimentally observed Tip/Stalk fractions at the same experimental VEGF input (see Methods section: Mathematical model of VEGF/NOTCH signaling).
Precise quantification of Tip cell spatial arrangement suggests disordered patterning in the engineered angiogenesis model
To enable the comparison between the modeling predictions and experimental observations, we first quantified the spatial patterning characteristics of the inferred positions of the Tip cells in the experimental angiogenesis model. As in our prior analysis using this experimental approach (7), we identified Tip cells based on their key phenotypic characteristic — invasive migration into the surrounding collagen matrix. As suggested above, Tip cells can either be present in the form of ‘mini-sprouts’ or be at the tips of sprouts containing recognizable lumens (see Fig. 3A and Table 1 for examples of this classification). Since formation of both mini-sprouts and lumenized sprouts involved emergence of Tip cells specified in endothelial cell monolayer lining the parental vessel, we constructed a two-dimensional map of the experimentally inferred spatial positions of Tip cells at the location of all mini-sprouts and sprouts (Figs. 3A & B). This mapping (see Methods section: Quantification of Tip-Tip cell distance in experiments) assumed that the Stalk cells found in the extending sprouts emerge through cell proliferation, rather by Stalk cell migration from the parental vessel. Experimentally, we used nuclear staining to identify non-Tip cells. Since the results above suggested that the cell fates and their patterns in our experimental setup were consistent with the ordered or somewhat disordered ‘salt-and-paper’ patterns, we further assumed that all the non-Tip cells adopted the Stalk fates (in the sense of Delta-low, NOTCH-high status, alternative to the Tip cell fate). The resulting map of experimentally specified Tip and Stalk cell locations was then used to calculate the shortest distances between Tip cells, measured in ‘cell hops’, i.e., the minimal number of intermediate cells between randomly chosen pairs of Tip cells (Fig. 3B). These distances included Stalk cells exclusively, and no intermediate Tip cells. If two or more Tip cells were at equal distance from a given other Tip cell, their distance ranking was assigned randomly (e.g., two Tip cells at the equal minimal distance to a given Tip cell, would be randomly assigned the ranking of the closest and second closest Tip cell). We then analyzed these data for the VEGFEXT= 100 ng/ml experimental input which resulted in the most robust sprouting, comparing the results with the predictions of our mathematical model for the same input level that, when averaging over multiple simulations, matched the experimentally measured fraction of Tip cells. Finally, we quantified the shortest paths separating Tip cells in the equilibrated patterns (Fig. 3C and Methods section: Quantification of Tip-Tip cell distance in modeling).
The processing of experimental data described above permitted a direct comparison of modeling predictions and experimental defined Tip cells locations, First, we examined the average distances from the Tip cells to the closest, second closest, etc. neighboring Tip cells. We found that this distance distributions closely agreed with the modeling predictions, particularly for the distances up to the 4th closest neighbor, differing substantially from the predictions for the ordered ‘salt-and-pepper’ patterns (Fig. 3D). A key finding was that, in agreement with the expectation from the disordered pattern model, there were frequent cases of direct contact between two Tip cells, making the average distance to the closest Tip cell around 0.5 cells in the model. In contrast, two Tip cells were always separated by at least one Stalk cell in “traditional” ‘salt-and-pepper’ models. Further analysis of the experimental distributions of Tip cell distances revealed that Tip cells were adjacent to at least 1 other Tip cell with 80% chance, and with at least 2 other Tip cells with 40% chance, and with at least 3 other Tip cells with 20% chance (Fig. 3E). These Tip cell distance distributions were again in agreement with the modeling results. Taken together these findings parodied strong evidence for the predicted partially disordered pattern of Tip cell specification.
Dynamic tracking of angiogenic cell fate specification
The integrative, computational and experimental, analysis presented above suggested that the spatial Tip cell distribution can be well explained by the model of a partially disordered ‘salt-and-pepper’ mechanism. However, it is not clear whether all such Tip cells would spearhead the formation of a new sprout, or retract back to an alternative (Stalk) cell fate. We addressed this question by dynamically tracking the fates of minisprouts to examine whether this state is an intermediate step towards the sprout formation. Specifically, we imaged the progress of sprouting in the same areas of a live parental vessel at different time points of 1 hour, 3 hours, 7 hours and 28 hours of incubation in 100 ng/ml of VEGF (Fig. 4A-H). Strikingly, we found that although all sprouts indeed formed from mini-sprouts, not all mini-sprouts persisted and initiated sprout formation. Instead, many mini-sprouts retracted and new mini-sprouts formed during the time-course of the analysis. We then tracked a group of 118 cells that adopted the mini-sprout phenotype at least once over a period of 28 hours after VEGF exposure. Their state change dynamics was visualized using the Sankey diagram (Fig. 4I). The initial state of any sprouts or mini-sprouts was classified as the Stalk cell to reflect the hypothesized ‘salt-and-pepper’ patterning structure, entirely consisting of either Tip or Stalk cells. When a mini-sprout retracted, it was newly marked as a Stalk cell. By the final time point of 28 hours of VEGF exposure, 45.8% of the cells that displayed the mini-sprout phenotype at least once during the experiment retracted back to the Stalk state, illustrating the highly dynamic phenotype of mini-sprout extensions and retractions. Of the remaining cells, 41.5% and 12.7% were classified to be either in the mini-sprout or sproutleading Tip cell states, respectively. Although sprout formation continued throughout the experiment, the rate of conversion of mini-sprouts to full-fledged sprouts gradually decreased over time, with 13.6%, 2.9%, and 7.5 % of mini-sprouts becoming sprout-leading Tip cells in the time ranges of 1-3, 3-7 and 7-28 hours. respectively (Fig. 4K-M). In most cases (86.7%), spouts emerged from newly formed mini-spouts (i.e., the cells that were Stalk cells and then mini-sprouts in the preceding two time points), suggesting that minispouts represent transient states rapidly converting to either the fully committed sprout state or to the Stalk state (Fig. 4O). These observations raised the question of what might define the commitment of a minisprout to the sprout differentiation. We next addressed this question by analyzing the spatial distribution of fully formed sprouts over the observed area of the parental vessel.
Random uniform model accounts for spatial distribution of extending sprouts
While the NOTCH/VEGF mathematical model could quantitatively resolve Tip-Stalk spatial patterns, it did not capture the rate of cell fate switching or explained the commitment of Tip cells to lead the formation of a mature sprout. To identify the underlying principles of sprout initiation, we thus integrated the multicell model with several alternative phenomenological hypotheses (summarized in Fig. 5A-D). We then tested these hypotheses against the measured distributions of distances between sprouts and, in particular, the observation that sprouts were always separated by at least one non-sprout cell (blue bars in Fig. 5E). In these testS, we ensured that the results were normalized to the overall density of sprouts observed experimentally (see Method section: Phenomenological models of Sprout selection). The first straightforward hypothesis was that mini-sprouts commit to the sprout phenotype independently of the location of other forming sprouts, constituting the $cell-autonomous sprout selection” model (Fig. 5A-B). In this case, however, the corresponding model predicted multiple contacts between sprouts (black line in Fig. 5E), in sharp contrast with the experimental observation. The observation that most sprouts are in contact with at least another Tip (Supplementary figure 2A), but never in contact with another sprout, suggested a control mechanism where sprout selection inhibits nearby Tip cells from committing to the same fate. This led to two additional alternative hypotheses. In $repulsion between sprouts” model, it was assumed that sprouts cannot be in contact; therefore, Tip cells cannot commit to the sprout phenotype if already in contact with a sprout (Fig. 5C). In the $random uniform” model, it was assumed that sprouts are selected randomly, but maximizing their overall spread in the lattice (Fig. 5D; see Method section: Phenomenological models of Sprout selection). While both models correctly predicted sprouts to never be in contact, the “random uniform” model better described the cases where adjacent sprouts are separated by two or more cells (Fig. 5E).
To test these mechanisms more rigorously, we computed the average distance (in cell numbers) between pairs of closest sprouts while also varying the number of sprouts allowed in the lattice (supplementary figure 2B-D), thus generating a curve of the typical sprout-sprout distancing as a function of sprout density in the lattice (Fig. 5F). The $random uniform” model predictions agreed very closely with the experimentally observed combination of sprout fraction and sprout-sprout distance, whereas the other two models greatly underestimated the distances between sprouts (Fig. 5F). Furthermore, while all models overestimated the fraction of the adjacent sprouts that are one cell away from the current sprout and underestimated the fractions of sprouts at greater distances, the deviation of the “random uniform” modelpredictions for this inter-sprout distribution was the lowest of all the models, again supporting the ‘random uniform’ model as the more likely to account for sprout selection (Fig. 5F).
Fibronectin distribution may mediate sprout induction
Random distribution maximizing the distance between sprouts is similar to allelopathy models, accounting to spatial dispersion of species maximizing distance between them. In these models, the key postulated mechanism is inhibition of growth of individuals of the same species through a mutual suppression mechanism(14-16). Another analogous set of mechanisms are embedded within the concept of the Turing pattern formation, the key to which is diffusible negative feedback regulator setting spatial distribution of morphogenic features (17, 18). A variant of such mechanisms is a model postulating depletion of some ingredient that is key to the local growth, by its active redistribution towards the growing pattern features and depletion from the zones between them, rather than active mutual inhibition of the pattern forming units.
Given these prior models, we hypothesized that a similar mechanism may account for the dispersion patterns of sprouts. We focused on the extracellular matrix as a possible medium accounting for the positive and negative pattern-setting interactions. Indeed recently, it has been observed that collagen can be reorganized by the growing sprouts, so that it is concentrated around the extending sprouts and depleted elsewhere(19-21). We explored whether a similar distribution is also be observed for fibronectin, an ECM component that is critical for the formation of lumenized sprouts (22-25). Fibronectin expression levels in the vicinity of individual cells comprising the parental vessel and the emerging sprouts was experimentally assessed by immunostaining, with simultaneous cell identification using induced cytoplasmic GFP expression and nuclear staining, followed by 3D reconstruction (Figs. 6A-H). Untreated quiescent cells (No treatment) and mini-sprouts showed similar levels of fibronectin expression (Fig. 6H). Interestingly, the fibronectin expression was highly enriched at the base but not the tip areas of the extending sprouts, suggesting that it may be a key determinant of sprout induction but not extension (Figs. 6B,C, & H).
We also examine the regional variation of fibronectin expression in larger areas, which was less variable and potentially more relevant to sprout extension. In particular, we accessed the ratio of cells having fibronectin levels higher than a threshold in groups of 7 cells around sprouts and mini-sprouts (Figs. 6I & J). The overall expression levels when all region types were combined was relatively uniform (Fig. 6J, third panel. Strikingly however, regions around sprouts (Fig. 6J, first panel) showed oppositely skewed patterns vs. mini-sprouts (Fig. 6J, second panel). Specifically, the fibronectin expression levels around sprouts were higher than the threshold, whereas the fibronectin levels around mini-sprouts were lower than the threshold. Altogether, these results supported the model in which fibronectin can indeed serve as a mediator of Turing-like induction of spouting patterns, through re-modeling that enriches it at the points of sprout induction and depletes it at the points where Tip cells (mini-sprouts) are not stabilized to form full-fledged, lumenized sprouting bodies.
A major challenge of the analysis of tissue development and homeostasis is understanding of how differentiation into distinct cell types can be robustly achieved, while also being sensitive to various pro-differentiation and morphogenic cues and, potentially affected by molecular noise in biochemical reactions. In the context of angiogenesis, this challenge more specifically relates to enabling effective vascular morphogenesis through robust yet environmentally responsive differentiation of endothelial cells into Tip and Stalk cells states. This process is guided by the multiple pro-angiogenic cues, such as VEGF and by the local tissue organization and mediated by the paracrine Delta-NOTCH cell signaling. Recent mathematical models of this process(26, 27) have considered the robustness of the Delta-NOTCH differentiation to molecular noise, concluding that noisy biochemical reactions can both disrupt and enhance the spatial differentiation patterns, depending on the magnitude and spatial distribution (26). The effect of pro-angiogenic cues, such as VEGF on robustness of spatial differentiation patterning remains substantially less explored. Furthermore, compiling between modeling predictions and experimental validation has been challenged by the complexity of in vivo angiogenesis analysis, both on cellular and molecular levels.
Here, we integrated engineered experimental angiogenic assay and a spatially resolved computational modeling analysis to explore the spatially and temporally resolved effects of VEGF on angiogenic cell speciation. Our results suggest that VEGF can have a dual role in inducing the initial Tip-Stalk cell differentiation. On the one hand, a low level of exogenous VEGF is essential to induce Delta-NOTCH signaling and the classical ordered ‘salt-and-pepper’ pattern, with approximately 25% of the cells adopting the Tip cell fate, as expected (28). This role of VEGF is conceptually similar to the classical case of EGF induced NOTCH signaling in C. elegance vulva development (29, 30). On the other hand, our results also suggest that an increase of VEGF levels can introduce disorder into this pattern, similar to order-disorder transition for various composite materials (12, 13), which may occur with increasing temperature and have the properties of a sharp phase transition. More specifically, high VEGF levels may play the role similar to an increased temperature in order-disorder transitions, leading to emergence of partially disordered ‘salt-and-pepper’ structures. These disordered structures are characterized by higher than expected fractions of Tip cells and, consequently, an increased occurrences of otherwise disallowed adjacent Tip cells. Importantly, for all VEGF input levels and the resulting spatial patterns, the Tip-Stalk cell specification continued to be robust, although the degree of induction of NOTCH signaling was dependent on the VEGF dose. These results suggested that cell specification patterns deviating from the expected ‘salt-and-pepper’ one can develop not only due to noisy NOTCH signaling, as suggested by prior models, but also due to the control of the fractions of alternative cell states by the magnitude the pro-differentiation cue.
In spite of the observation that disordered ‘salt-and-pepper’ patterns still display robust differentiation, the spatial patterns of cell co-localization can generate inherent instabilities, e.g., due to two adjacent Tip mutually suppressing their fate selection through NOTCH signaling. Instabilities of this sort may increase the sensitivity of the differentiation process to additional cues and can also lead to facilitated dynamic switching of cell fates (e.g., Tip to Stalk and vice versa) over prolonged periods of time. Such instabilities might lead to oscillatory-like fluctuations of NOTCH signaling as observed in other differentiation processes (31-33) and in endothelial cell sheets under high VEGF concentration inputs(34). Importantly, these instabilities may also underlie the striking observation of the continuous retraction and extension of mini-sprouts (protruding Tip cells) observed in our study. This dynamic fate-switching behavior can thus represent a signature of an unstable differentiation process that may either stabilize, in response to additional cues, leading to a specific morphogenetic outcome, such as the extension of a stable sprout, or display a prolonged instability resulting in a lack of pronounced morphogenesis. Such poised but unstable states may be similar to the undifferentiated state of neurogenic progenitors displaying oscillatory NOTCH signaling, which can proceed to differentiation after the oscillation is resolved into a temporally stable NOTCH activity (35).
The cues stabilizing cellular differentiation and morphogenesis can vary and represent the signature of both global and local pro- and anti-angiogenic environments. For instance, our prior analysis indicated that exposure of model parental vessels to VEGF only rather than a more complete pro-angiogenic cocktail of various factors, can result in formation of mini-sprouts (and hence, effective Tip-Stalk cell differentiation), but not full-fledged sprouts (7). Sprout formation may also be modulated by the presence of mural cells and pro-inflammatory cytokines, which can indirectly modulate the NOTCH activity, but could also have additional effects, serving to stabilize a specific differentiation outcome. However, even if sprouts do form, it is not clear how their spatial distribution may arise and be potentially controlled by the environmental inputs. Our results argue that, for a given number of sprouts forming in the parental vessel, their mutual distances are maximized. This spatial distribution is consistent with a Turing-like mechanism (17, 18), implying the existence of a long-distance interaction inhibiting formation of new sprouts in the vicinity of the existing ones. Although the actual mechanism of putative Turning-like pattern formation is not fully elucidated here, our results are consistent with a variant of this regulatory behavior, in which a component of the extracellular matrix, fibronectin is actively redistributed by the nascent sprouts, with this ECM component being enriched at the points of sprout formation, but depleted In other zones, thus preventing sprout induction in the depleted areas. This redistribution is indeed equivalent to the classical Turing mechanisms that would involve generation of an explicitly inhibitory compound by the growing sprouts. Fibronectin has been implicated as a key pro-angiogenic ECM component, possibly due to its integration with VEGF, which further increases the plausibility of this mechanism (23). Interestingly, we find elevated levels of fibronectin at the bases of extending sprouts, but not at their tips. This finding has two implications. First, fibronectin may be an important factor in the sprout induction rather than extension, hence the growing sprout can progress into the surrounding matrix beyond the area of enriched fibronectin, leaving it behind. Secondly, fibronectin can also promote lumen formation (24, 25), thus its enrichment at the bases of extending sprouts can further contribute to their lumenized structure. Furthermore, this mechanism can help explain the dramatic influence of cytokines, such as TNF, in preventing sprout formation, which can happen in sharp, TNF dose-dependent manner (7). Indeed, TNF avidly binds to fibronectin (22) and therefore, at high enough concentrations, would have a particular anti-angiogenic effect in the areas of increased fibronectin density, which according to the proposed mechanism would be the areas of incipient sprouts. This would dramatically increase its anti-sprouting effect, even if the effect of this cytokine on the NOTCH-dependent cell specification is more muted (7). Of note, another version of the Turing mechanism has recently been suggested to account for the branching of the sprouts, also involving formation of new Tip cells leading individual branches, although the molecular mechanism postulated in that analysis was distinct from the one proposed here (36, 37). These models, though plausible, will need to be further tested to ascertain causality of the proposed mechanisms, although at this stage the Turing-like mechanisms appear to be the best candidates to explain the experimental results we obtained.
Overall, our analysis supports the following dynamic view of angiogenic induction. The VEGF input can induce NOTCH signaling and formation of Tip cells that can behave as mini-sprouts. At lower VEGF input, a more ordered pattern of Tip cell induction can lead to formation of approximately 25% of Tip cells, but relatively few of these will become sprouts, reflecting lower sensitivity to additional ambient cues, promoting sprout formation. On the other hand, at higher VEGF inputs, a greater disorder of Tip cell patterning permits higher sensitivity to external cues, such as fibronectin, that can stabilize the sprouting. In addition, a more frequent co-localization of Tip cells under this condition enables initiation of a sprout from two adjacent cells, as observed in a fraction of cases in our experiments (the case in Table 1 involving formation of a sprout by two adjacent cells). The initial emergence of sprouts leads to a progressively less likely sprout formation and to the overall maximization of the distance between the sprouts. Both these observations are consistent with non-local inhibition of sprout formation around the sprouts that have already formed. A plausible mechanism for this inhibition and the overall pattern formation is the re-distribution of fibronectin from the zones between sprouts towards the incipient sprouts, constituting both a positive and negative feedback loops, commonly assumed in a variant of the Turing patterns from action. This mechanism is very sensitive to various local inputs distinct from VEGF and fibronectin, which can further influence the location and density of the developing blood vessels. These may include the effects of pericytes or local inflammatory environments, as mentioned above, but also other ECM components, such as collagens, that may enhance the protrusion of Tip cells and thus stabilize the emergent sprouts both directly and indirectly. Endothelial fate induction process may be interesting to contrast with other complex multicellular processes, including collective epithelial migration, where NOTCH signaling similarly modulates fine-grained patterns of leader and follower cells (38), and underscore the need in the future to develop more refined models that explicitly integrate the interconnections between biochemical and mechanical regulation of Tip-Stalk fate(39).
The analysis presented here highlights the utility of combining experimental engineered vasculature models with mathematical analysis as integrated research platforms to gain a progressively better insight into angiogenesis in highly controlled micro-environments. Although, questions remain about the mechanistic underpinnings of the phenomena observed here and in related studies, the analysis enabled by these model systems can help formulate guiding hypotheses for further understanding of angiogenesis in vivo, while decoupling the complexities of the native angiogenic environments. We anticipate that the dynamic exploration presented here can help pave the way for further quantitative understanding of this key biological process.
Materials and Methods
Chips for mimicking 3D angiogenesis in vitro were fabricated as introduced in our previous work(7). Fluorescence images of the in vitro model were acquired by Lecia scanning disk confocal microscopy, and the z-stack images were processed with IMARIS (Bitplane) to quantify Tip-Tip distance, to track the positions of sprouts and mini-sprouts, and to analyze fibronectin intensity distribution. We generalized existing mathematical models of the interconnected signaling between the NOTCH and VEGF pathways to a two-dimensional multicellular scenario (7, 40). The temporal dynamics of NOTCH (N), Delta (D), Jagged (J), NOTCH intracellular domain or NICD (I), and VEGF receptor (VR) in a cell were modeled with the parameter values presented in Table 2. The details of materials and methods can be found in SI Appendix, Materials and Methods.
This work was supported by NIH through NCI U54 CA209992 (to A.L.); Mia Neri Foundation (to T.-Y.K.); The Center for Theoretical Biological Physics sponsored by the NSF (Grant PHY-2019745), NSF-PHY-2210291, CPRIT Scholar in Cancer Research sponsored by the Cancer Prevention and Research Institute of Texas (to J.N.O.); a Simons Foundation grant 594598 (to Q.N.). F.B. was supported by a National Science Foundation grant DMS1763272 (Q.N.).
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