Computational and Systems Biology

Latest articles

1. 1. Computational and Systems Biology

   Protein Overexpression: Reaching the limit

   Benedetta Bolognesi, Ben Lehner

   How many copies of a protein can be made before it becomes toxic to the cell?
2. 1. Computational and Systems Biology

   Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins

   Yuichi Eguchi et al.

   Measurement of expression limits of yeast glycolytic proteins reveal the protein burden limit, which is the expression limit of any protein in the cell.
3. 1. Cell Biology
   2. Computational and Systems Biology

   Multiple inputs ensure yeast cell size homeostasis during cell cycle progression

   Cecilia Garmendia-Torres et al.

   Yeast cell size homeostasis is not controlled by a G1-specific mechanism alone but is likely to be an emergent property resulting from the integration of several mechanisms that coordinate cell and bud growth with division.
4. 1. Cell Biology
   2. Computational and Systems Biology

   A quantitative approach for analyzing the spatio-temporal distribution of 3D intracellular events in fluorescence microscopy

   Thierry Pécot et al.

   QuantEv is a fully automatic and semi-parametric method that allows quantitative analysis of the spatio-temporal distribution of complex molecular trafficking objects at the scale of the whole cell.
5. 1. Cancer Biology
   2. Computational and Systems Biology

   Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes

   Jia-Ren Lin et al.

   t-CyCIF can be used to collect spatially-encoded, multiparametric data from fixed and embedded research or clinical specimens making it possible to probe the organization of tumors and tissues at a single-cell level.
6. 1. Cell Biology
   2. Computational and Systems Biology

   A positive-feedback-based mechanism for constriction rate acceleration during cytokinesis in Caenorhabditis elegans

   Renat N Khaliullin et al.

   Positive feedback between contractile ring myosin and compression-driven cortical flow can explain the exponential accumulation of contractile ring components and constriction rate acceleration that ensures timely cell separation during cytokinesis.
7. 1. Chromosomes and Gene Expression
   2. Computational and Systems Biology

   Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells

   Raghuvir Viswanatha et al.

   Pooled CRISPR knockout screening in Drosophila cells enables high-resolution, genome-wide functional genomic comparisons in cell-lines across vast evolutionary distance.
8. 1. Computational and Systems Biology
   2. Physics of Living Systems

   Biophysical clocks face a trade-off between internal and external noise resistance

   Weerapat Pittayakanchit et al.

   Molecular clocks that are robust to external fluctuations are vulnerable to internal fluctuations and vice versa.
9. 1. Biochemistry and Chemical Biology
   2. Computational and Systems Biology

   Coevolution-based inference of amino acid interactions underlying protein function

   Victor H Salinas, Rama Ranganathan
10. 1. Computational and Systems Biology
    2. Developmental Biology

    Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension

    Timothy E Vanderleest et al.

    Tricellular vertices possess sliding behaviors that harness radial forces and drive cell shape changes and intercalation in an epithelial tissue.