Structural variants (SVs) are a powerful source of genetic variation that fuels the evolution of genomes and species (Gorkovskiy and Verstrepen, 2021). SVs regroup a wide variety of large- scale sequence alterations that can have profound consequences for organismal phenotypes and genome evolution. For example, aneuploidies can underlie the emergence of antifungal resistance in fungal pathogens (Selmecki et al., 2006) and drive tumorigenesis in mammalian cells (Shoshani et al., 2021; Trakala et al., 2021). Polyploidization is frequently linked to speciation and adaptation, notably in plants (Otto and Whitton, 2000). Mitotic recombination can yield loss of heterozygosity (LOH) events that drive adaptation in heterozygous hybrid genomes (Smukowski Heil et al., 2017), but also the somatic fixation of oncogene mutations (Cavenee et al., 1983). Repeated sequences are known to play a leading role in the generation of SVs (Lower et al., 2019). Notably, transposable elements (TEs) are sequences capable of semi-autonomous replication, producing families of repeats spread within genomes (Bourque et al., 2018). Because of their replicative nature, TEs are major generators of SVs: not only do they create interspersed duplications of themselves, but they can also trigger genome rearrangements through ectopic recombination (Dunham et al., 2002; Petrov et al., 2003). While TEs can cause SVs, various types of SVs can also affect the genomic landscape of TEs by producing gains and losses of individual copies (Figure 1).

Examples of how SVs can impact the genomic landscapes of TEs in hybrids.

An ancestral heterozygous genome is shown as an example, with two subgenomes (black and gray) harboring TEs (green boxes) in distinct copy numbers and at different positions. Examples of various types of SVs are shown in derived genomes: polyploidy (gain of haploid sets of chromosomes), aneuploidy (gain or loss of whole chromosomes), loss of heterozygosity (LOH, inter-homolog conversion of chromosome segments), and de novo transposition. The corresponding changes in TE copy numbers are shown at the right.

TEs are inherent components of virtually all Eukaryotic genomes and owe their evolutionary success to the fitness advantage of their own replicative ability. However, this selfish propagation conflicts with the fitness of the host, and can have profound deleterious impacts (Bucheton et al., 1984; Pasyukova et al., 2004; Wilke and Adams, 1992). The abundance of TEs per genome (or TE load) is an important population genetic parameter, as it is expected to correlate with the fitness cost for the host. TE load in a population is most often modeled as a quantity reflecting the balance between the gain of novel copies by transposition, and the decrease in copies by loss (e.g. by excision or sequence degeneration) and purifying selection against insertion alleles (Charlesworth et al., 1994; Charlesworth and Langley, 1989). However, TE load is not only defined by the copy number (CN) of TEs in the genome, but also where TE insertions fall and, consequently, how they interact with neighboring host sequences. The multidimensional nature of TE load makes its full characterization a complex task. Recent advances in long-read sequencing make it increasingly possible to decompose TE load by resolving the specific genomic context in which TE insertions are found (Oggenfuss and Croll, 2023; Rech et al., 2022). Additionally, accurate genome resolution opens unprecedented opportunities for a comprehensive assessment of the role of SVs in shaping TE load.

One long-standing hypothesis postulates that hybridization can cause a genomic shock that triggers TE mobilization and increases TE load in hybrids (McClintock, 1984), with major implications for speciation and hybrid evolution. We previously tested this hypothesis in Saccharomyces yeast hybrids by performing a large-scale evolution experiment by mutation accumulation (MA) to investigate the near-neutral evolution of TEs in hybrid genomes (Hénault et al., 2020). Saccharomyces cerevisiae genomes harbor five main long terminal repeat (LTR) retrotransposon families named Ty1-Ty5 (Kim et al., 1998). Its undomesticated sister species Saccharomyces paradoxus comprises related families Ty1, Ty3 and Ty5 (Yue et al., 2017), in addition to Tsu4 which was horizontally transferred from Saccharomyces uvarum (Bergman, 2018). We generated a collection of MA lines comprising a set of 11 hybrid genotypes created by crossing wild strains from North American populations of S. paradoxus and S. cerevisiae (Charron et al., 2014a; Kuehne et al., 2007; Leducq et al., 2016, 2014) that are separated by a range of evolutionary divergence, and by replicating each cross many dozen times independently (Charron et al., 2019; Hénault et al., 2020). The resulting collection was evolved by subjecting each line to periodical extreme bottlenecks by streaking for single colonies on plates of rich medium for ∼770 mitotic generations, thus amplifying the power of random genetic drift. We previously sequenced these MA lines with short reads and measured the changes in Ty load after evolution by producing aggregate estimates of relative abundance for each family, and found no support for the TE reactivation hypothesis (Hénault et al., 2020). However, the use of short reads alone precluded the detailed resolution of hybrid genomes and the decomposition of their Ty load. For instance, different SVs (including de novo transposition) may have changed Ty CNs in opposing directions, yielding no significant change in net CN.

While producing collapsed assemblies for haploid or predominantly homozygous genomes is now relatively straightforward, complex heterozygous genomes are much more difficult to characterize. Recent studies demonstrated the feasibility of the approach (Heasley and Argueso, 2022; O’Donnell et al., 2022), but the task of resolving hybrid genomes across a range of parental divergence under a unified comparative framework remains challenging. Here, we design a long- read sequencing, phasing and assembly approach to resolve hybrid MA lines genomes. We find that de novo transposition events play a minor role in shaping the Ty load in comparison to other SV types. Since the number of de novo insertions gained during MA is low, we further dissect transposition rate variation in S. paradoxus hybrids at a higher resolution with in vivo transposition rate measurements. We identify many aspects of hybrid genotype specificity that shape transposition rates, including initial Ty load, Ty sequence variation and mitochondrial DNA (mtDNA) inheritance.


The eleven MA crosses (Charron et al., 2019; Hénault et al., 2020) comprise five crosses within S. paradoxus populations (CC, within SpC; BB, within SpB), four crosses between S. paradoxus populations (BC, SpBSpC; BA, SpBSpA), and two interspecific crosses (BSc, S. paradoxus SpBS. cerevisiae) (Figure 2A). We previously selected 127 independently evolved MA lines (10- 12 per cross) and sequenced their genome with Oxford Nanopore long reads (Hénault et al., 2022). To produce an accurate representation of the two distinct subgenomes of each evolved MA line, we designed a strategy consisting of 1) sorting long-read libraries using variants that discriminate the two parental genomes of each cross, and 2) performing separate de novo assembly on the sorted reads. The proportion of sequenced bases successfully classified into subgenomes varied substantially depending on the cross (Figure 2B). CC crosses had the lowest classification rate (median: 70.8%) due to the scarcity of variants that discriminate SpC genomes from each other. BB crosses had a median classification rate of 84.5%, while the remainder (BC, BA and BSc) reached a higher rate (median: 93.7%). Unclassified reads tended to be shorter than classified reads, but the difference was narrower for libraries with lower classification success (Supplemental Figure S1). The scarcity of discriminating variants in low-divergence crosses is likely causing the classification of increasingly longer reads to fail. However, unclassified reads showed no overrepresentation in specific genomic regions (Supplemental Figure S2) and their exclusion is thus unlikely to bias the resulting assemblies.

Subgenome-level resolution of hybrid yeast MA lines genomes using a long-read phasing and assembly approach.

(A) Design of the MA crosses. Haploid derivatives of wild isolates used as parental strains for the MA crosses are shown on the Y-axis. Strains in bold are shared by multiple crosses. MA crosses are shown on the X-axis (left to right, from the least to the most divergent), with the number of lines randomly sampled for long-read sequencing shown in parentheses. The phylogenetic tree displays the evolutionary relatedness between wild populations of S. paradoxus and S. cerevisiae, based on genome-wide nucleotide variants (scale bar: substitutions per site) (Hénault et al., 2022). (B) Summary of the classification of long reads in two parental subgenomes. For each MA line library, the percent of sequenced bases classified as the MATa and MATα parental subgenomes are shown on the X and Y axes, respectively. Dotted lines indicate global classification rates. Full lines indicate expected subgenome classification ratios of 1:1 for diploid (circles) or tetraploid (squares) lines, and 2:1 or 1:2 for triploid (triangles) lines. (C) Summary statistics of the de novo subgenome-level assemblies of the MA lines.

Despite the variation in classification rates, the classified reads yielded highly contiguous subgenome-level assemblies, with a median of 22 contigs, a median N50 of 783 kb, and 95% of the assemblies having N50 values larger than 200 kb (Figure 2C, Supplemental Table S1). Only six assemblies were more fragmented (>100 contigs) and corresponded to the sorted libraries with the least sequenced bases (Supplemental Figure S3A), which are expected to complicate the assembly. Thus, our approach yielded chromosome-level representations of the two subgenomes of each MA line.

We then integrated various data to infer the state of all loci containing Ty elements in the MA lines subgenomes in relation to the corresponding parental genome. First, we produced whole-genome assemblies of the 13 haploid parental strains from long reads. We annotated their Ty elements and used whole-genome alignments to determine the orthology of Ty loci between the parental strains of each cross (Supplemental Figure S4). Only 10 pairs of loci were found to be orthologous in four of the lowest divergence crosses (CC1: 1, CC2: 5, CC3: 3, BB1: 1). We also annotated the Ty elements in the MA lines subgenome assemblies and defined the orthology between Ty loci by aligning subgenome assemblies against the corresponding parental assembly. We also computed the depth of coverage of sorted long-read libraries mapped on the corresponding parental genome assemblies to yield estimates of CN per genomic window. We then applied a decision tree on this data to classify individual Ty loci by type of SV (Supplemental Figure S5). 89.8% of Ty loci were successfully classified, with low-contiguity assemblies contributing disproportionately to classification failure (Supplemental Figure S3B). From the successfully classified loci, 79.0% had no change compared to the parental genomes. The remainder had an uneven distribution of SV classes (Figure 3A). The largest class was polyploidy, since many lines within CC and BC crosses have become tetraploid or triploid (Charron et al., 2019; Marsit et al., 2021). Triploidization was exclusive to six BC lines and was likely caused by the diploidization of SpC parental cells (creating mating-competent pseudohaploids) prior to hybridization (Charron et al., 2019), meaning that triploidization occurred before MA strictly speaking. LOH and aneuploidy also accounted for large fractions of Ty loci affected by SVs. Notably, there was a substantial impact of LOH events in low-divergence CC crosses. Ty1 is the most abundant family in SpC genomes and despite a relatively constant load, Ty1 copies mostly occupy non-orthologous loci (Supplemental Figure S4A), providing the substrate for LOH to affect Ty1 landscapes in CC crosses. While LOH had a bidirectional effect on Ty CNs, causing gains and losses in approximately equal proportions, aneuploidies had a strong bias towards gains (Figure 3B). The distributions of net Ty CN change per MA line showed that most crosses had significant gains (Figure 3C). The post-evolution Ty CNs quantified from relative depth of coverage of short-read libraries (Hénault et al., 2020) and the net CN changes determined here were globally correlated (mixed linear model with random intercepts for MA cross✕Ty family combinations, P- value=3.01✕10-35). In many individual cases, significant positive correlations were observed (Supplemental Figure S6), indicating that our current analyses recapitulate previous results.

Distribution of the number of Ty loci affected by various SV classes.

(A) Number of Ty loci affected by SVs per line for each cross. Dots represent individual MA lines and bars show the average count per cross. Polyploidy refers to increased ploidy level to triploidy or tetraploidy from the expected diploid initial state. LOH refers to the conversion of large chromosome segments (interstitial or terminal) from one parental genotype to the other. Aneuploidy refers to gains or losses of whole chromosomes. Truncation refers to partial deletion of a full-length Ty copy. De novo refers to the presence of a new full-length Ty copy not found in the parental background or any other MA line. Deletion refers to the local deletion of a full-length Ty copy without other SVs. Excision refers to the replacement of a full-length Ty copy with a matching solo LTR, consistent with intra-element LTR-LTR recombination. (B) Average gain and loss of Ty CN per line for each cross, for SV classes that can have bidirectional effects on Ty CN. (C) Distributions of net Ty CN change for individual MA lines. Symbols indicate FDR-corrected P- values for Wilcoxon signed-rank tests (*: p≤0.05, **: p≤0.01).

We identified 23 candidate de novo Ty insertions as loci occupied by one full-length Ty element that was absent from the corresponding parental genome and any other MA line. From these candidates, we used strict filtering criteria to narrow down confident loci that were compatible with retrotransposition. First, we required the presence of characteristic target site duplications (TSDs) of 5-6 nt (Supplemental Figure S7), consistent with the staggered dsDNA cut catalyzed by the Ty1 integrase (Wilhelm et al., 2005). We further confirmed the uniqueness of Ty insertion junction sequences by inspecting whole-chromosome alignments comprising the MA line subgenomes and all parental genomes. We also ensured that all de novo TSD sequences were unique and absent from the parental full-length Ty loci.

We determined that 18 de novo loci were compatible with all these criteria (Figure 4). The quantitative impact of de novo insertions on Ty load evolution is thus minor in comparison to other SV types, with 0.14 insertions per line on average (Figure 3A). All de novo loci belonged to the Ty1 family of S. paradoxus. Eight out of 11 crosses exhibited de novo Ty1 retrotransposition events, half of them being found in the CC crosses. Eight insertions were found at loci with parental Ty1 elements (full-length or solo-LTR) in close proximity, highlighting the importance of the de novo assembly approach to accurately resolve these complex loci. Two retrotransposition events in BC2 lines inserted into the UWOPS-91-202 SpB subgenome that is natively devoid of full-length elements, effectively re-colonizing a genome in which all Ty families had gone extinct. We built a maximum likelihood phylogenetic tree comprising the de novo insertions and all parental Ty1 and Ty2 full-length sequences (Supplemental Figure S8). This analysis revealed that de novo copies originated from major Ty1 clades populating SpC, SpB and SpA genomes, indicating that Ty1 is globally active in S. paradoxus populations. However, this tree had generally poor branch support values from the bootstrap analysis. A phylogenetic network built from the same dataset showed substantial signal for reticulate evolution between Ty1 families of S. cerevisiae and S. paradoxus SpA (Supplemental Figure S9), in agreement with previous studies that showed interspecific horizontal transfers (Bleykasten-Grosshans et al., 2021; Czaja et al., 2020).

De novo retrotransposition events in the MA lines.

Genome maps show the full- length Ty elements annotated in the subgenome assemblies of the MA lines that have de novo insertions. Gray boxes represent chromosomes, which are numbered at the figure top. Empty triangles represent the parental annotations present at a CN greater or equal to one. Full triangles correspond to the confident de novo retrotransposition events. Triangle orientation indicates whether the annotation is on the plus (right pointing) or minus (left pointing) chromosome strand. The cross corresponding to each MA line is labeled at the right, with the total count of de novo retrotransposition events shown between parentheses.

The sequence resolution of de novo loci also enabled the characterization of insertions that disrupted parental genomic features. One de novo Ty1 element inserted into the left LTR of a parental full-length Ty1 copy in the MSH-587-1 subgenome of the MA line J27 (CC1 cross, Supplemental Figure S10A), resulting in a nested insertion. Additionally, in a single case, a de novo Ty1 element inserted 37 bp downstream of the start codon of the host gene AIM29 in the LL2011_009 subgenome of the MA line L41 (CC3 cross, Supplemental Figure S10B), introducing an early stop codon that yields a peptide of 36 amino acid residues.

The frequency of de novo Ty1 retrotransposition events per cross tends to support our previous conclusion that TE mobilization does not scale positively with parental divergence in Saccharomyces hybrids (Hénault et al., 2020). A similar conclusion was reached from interspecific hybrids between S. cerevisiae and the more distantly related species S. uvarum (Smukowski Heil et al., 2021). However, the number of de novo Ty1 insertions characterized by MA are quite low (at most four per cross) making it difficult to confidently test for differences in transposition rate between hybrid genotypes. Multiple aspects of natural genetic variation were shown to impact the mobilization of Ty1 (Czaja et al., 2020; Smukowski Heil et al., 2021), suggesting that some of these aspects could also contribute to modulate transposition rate in S. paradoxus hybrids.

We investigated how multiple aspects of genotype specificity impact transposition rate in S. paradoxus hybrids using an existing genetic assay developed in S. cerevisiae to measure the retrotransposition rate of a single tester Ty1 element (Curcio and Garfinkel, 1991). This assay relies on an antisense artificial intron (AI) that interrupts a copy of the HIS3 gene, which is itself inserted in antisense orientation at the 3’ end of the internal ORF of a plasmid-borne Ty1 element. The correct splicing of the AI is enabled by Ty1 transcription, following which the reverse transcription of the Ty1 mRNA and the integration of the resulting dsDNA copy in the genome restores the HIS3 ORF. Selecting for histidine prototrophy in a fluctuation assay (Luria and Delbrück, 1943) enables the estimation of the transposition rate of the tester element. We adapted this assay to measure retrotransposition rates in multiple S. paradoxus backgrounds, and with two Ty1 sequence variants sampled from SpB and SpC genomes as tester elements (Supplemental Figure S11, Supplemental Tables S2-6).

A mechanism of negative copy-number dependent self-regulation of retrotransposition was characterized in the Ty1 family of S. cerevisiae, termed copy-number control (CNC) (Garfinkel et al., 2003). This mechanism relies on the expression of an N-truncated Ty1 Gag protein (p22) from two alternative downstream start codons (Nishida et al., 2015; Saha et al., 2015). p22 interferes with the assembly of the viral-like particles essential for Ty1 life cycle completion (Cottee et al., 2021; Saha et al., 2015). CNC yields a steep negative relationship between the retrotransposition rate measured with a tester element and the number of p22-expressing Ty1 copies in the genome (Garfinkel et al., 2003). We previously observed a negative relationship between Ty1 load change after MA and the initial Ty1 load, and hypothesized that CNC-like regulation could be at play (Hénault et al., 2020). While the scarcity of Ty1 mobilization in MA lines now refutes this hypothesis, transposition rates could still be impacted by the initial load. We measured transposition rates for Ty1 elements in eight different haploid backgrounds of S. paradoxus (one SpA, four SpB, and three SpC; Supplemental Tables S5,7) that exhibit a wide variation in genomic Ty1 CNs. The transposition rate in SpB backgrounds had a strong negative relationship with Ty1 CN (Figure 5). The transposition rate in a SpB background with a single Ty1 copy (UWOPS-79- 140, estimated CN: 1.2) reached 1.73✕10-6 cell-1 gen-1. In comparison, an SpB background harboring a single additional Ty1 copy (MSH-604, estimated CN: 2.2) had a transposition rate over an order of magnitude lower (9,77✕10-8 cell-1 gen-1). In SpC backgrounds, which all have higher Ty1 CNs (20.7-32.4), the transposition rate was below 1✕10-8 cell-1 gen-1.

Ty1 transposition rate as a function of chromosomal Ty1 CN in S. paradoxus.

Transposition rates were measured in eight haploid S. paradoxus backgrounds, highlighted by background gray bars at the corresponding CN. Black bars indicate the 95% confidence intervals. Empty symbols represent upper bound transposition rate estimates obtained by artificially inflating the mutant count by 1 when 0 was observed. Marker shapes indicate the sequence variant of the tester Ty1 element, either SpB (circles) or SpC (squares). The S. paradoxus backgrounds and estimated Ty1 CNs are, from left to right: UWOPS-79-140 (SpB, 1.2); MSH-604 (SpB, 2.2); LL2012_021 (SpB, 4.6); LL2012_014 (SpB, 10.6); YPS744 (SpA, 14.4); LL2012_011 (SpC, 20.7); LL2011_012 (SpC, 26.3); LL2011_009 (SpC, 32.4).

In each background, we tested two sequence variants of the tester Ty1 element: one derived from a SpB genome (MSH-604), and one derived from a SpC genome (LL2011_012). We found that the SpC variant generally yielded lower transposition rates (Figure 6A). In the UWOPS-79-140 SpB background, the transposition rate of the SpB variant (1.73✕10-6 cell-1 gen-1) was significantly higher than the SpC variant, with a difference of over threefold (5.34✕10-7 cell-1 gen-1). We crossed three of the haploid backgrounds, one from each population (SpB: UWOPS-79-140, SpC: LL2011_012 and SpA: YPS744), to generate all the possible homozygous and heterozygous diploid backgrounds (Supplemental Table S6), and measured their transposition rates (Figure 6B, Supplemental Table S8). This experiment confirmed that transposition rates are lower with the SpC Ty1 variant, with the difference being significant in three different backgrounds (SpASpB, SpBSpB and SpBSpC). Additionally, these measurements showed that transposition rates in heterozygous hybrids are intermediate between their respective homozygous parental backgrounds (Supplemental Figure S12), providing further evidence for the robustness of transposition regulation in hybrids (Hénault et al., 2020; Smukowski Heil et al., 2021).

Ty1 transposition rate as a function of the sequence variant of the tester Ty1 element.

Transposition rates of the SpB (X-axis) and SpC (Y-axis) Ty1 variants are shown for haploid backgrounds (A) and diploid backgrounds (B). Black bars indicate the 95% confidence intervals. Empty symbols represent upper bound transposition rate estimates obtained by artificially inflating the mutant count by 1 when 0 was observed. The diagonal line indicates a 1:1 relation between transposition rates for the SpB and SpC Ty1 variants.

Like many other fungal species, mtDNA inheritance in Saccharomyces yeasts is biparental (Wilson and Xu, 2012), yielding a transient state of heteroplasmy that is resolved with the fixation of a single mtDNA haplotype by vegetative segregation (Birky, 2001). A recent study measured the rate of Ty1 transposition in S. cerevisiaeS. uvarum hybrids while controlling mtDNA inheritance and showed an effect of the parental mtDNA haplotype (Smukowski Heil et al., 2021). The measured effect was quite strong, the transposition rate linked with the S. uvarum mtDNA haplotype being approximately one order of magnitude lower than the S. cerevisiae mtDNA haplotype. We investigated whether mtDNA inheritance also affected transposition rates at the intraspecific level in hybrids between S. paradoxus natural populations. For each of the diploid crosses described above, we tested reciprocal crosses with either parental haploid background being depleted of its mtDNA (ρ0). As expected, the reciprocal ρ0 parents in the homozygous SpC background yielded no significant difference in transposition rate (Figure 7). However, many significant differences were observed in heterozygous backgrounds. In both SpASpC and SpBSpC hybrids with the SpB Ty1 tester variant, the transposition rate was significantly lower when the mtDNA was inherited from the SpC (MATα) parent (Figure 7). Although not statistically significant, the same trend was observed for the SpC Ty1 tester variant in the SpBSpC hybrid. These results indicate a moderate but consistent effect of the SpC mtDNA in lowering Ty1 transposition rate.

Ty1 transposition rate as a function of the inherited mtDNA haplotype.

Transposition rates in diploid backgrounds as a function of whether the inherited mtDNA is that of the MATa parent (X-axis) or the MATα (Y-axis) are shown. Black bars indicate the 95% confidence intervals. Empty symbols represent upper bound transposition rate estimates obtained by artificially inflating the mutant count by 1 when 0 was observed. Marker shapes indicate the sequence variant of the tester Ty1 element, either SpB (circles) or SpC (squares). The diagonal line indicates a 1:1 relation between transposition rates for reciprocal mtDNA inheritance. Colored arrows at the bottom left indicate the mtDNA haplotype that would correspond to a higher transposition rate on that side of the diagonal for each hybrid background.


The repetitive nature of TEs makes them notoriously difficult to resolve in genomes (Goerner- Potvin and Bourque, 2018). As such, studies investigating the TE reactivation hypothesis in hybrids have used a variety of methods for quantifying the activity of TEs at different levels, for instance in terms of genomic abundance (O’Neill et al., 1998; Ungerer et al., 2006) or expression level (Dion-Côté et al., 2014; Renaut et al., 2014). Not all methods enable the decomposition of TE load at the level of sequence-resolved insertion loci (Tusso et al., 2021), which is an especially challenging task in heterozygous genomes. A full mechanistic understanding of how TE load evolves in hybrids requires the ability to characterize how TE copies are gained and lost, through the action of de novo transposition and other types of SVs.

Here, we use long-read sequencing to yield accurate representations of the genomes of yeast hybrids experimentally evolved under a near-neutral MA regime, and investigate the mechanisms responsible for changes in the genomic landscape of Ty elements. We find that SV types like polyploidy, LOH and aneuploidy have the largest impact on Ty CN variation. While de novo Ty1 insertions compatible with retrotransposition were found in most of the MA crosses, their contribution to CN changes is very modest compared to the transposition-unrelated SVs. The scarcity of de novo insertions in the MA lines provides insufficient resolution to dissect subtle transposition rate variation between hybrid genotypes. We thus complement the genomic analysis of MA lines with in vivo Ty1 transposition assays to measure transposition rate in multiple genetic backgrounds. We find that initial Ty1 load, Ty1 sequence variation and mtDNA haplotype inheritance are factors that modulate transposition rates within S. paradoxus. In addition, our results confirm that unlike other yeasts (Tusso et al., 2021), Ty mobilization in Saccharomyces is not triggered by hybridization (Hénault et al., 2020; Smukowski Heil et al., 2021) and this, along a wide gradient of parental evolutionary divergence.

Our in vivo transposition assays revealed sharp contrasts in the dynamics of Ty1 mobilization. Multiple aspects of the SpC genotypes are associated with lower mobilization rates. Ty1 transposition occurs at a very low rate in SpC backgrounds compared to other S. paradoxus populations, and using a SpC Ty1 sequence variant as a tester element showed a much lower transposition rate compared to a SpB Ty1 variant in matched backgrounds. Moreover, reciprocal crosses with controlled mtDNA inheritance suggested an effect of SpC mtDNA in reducing Ty1 transposition rates. Despite the clear evidence for lower mobilization in vivo, half of the de novo transposition events in the MA lines genomes happened in intra-lineage SpC crosses. The SpC population harbors a low level of genetic diversity compared to SpB (Eberlein et al., 2019; Leducq et al., 2016), and have a fairly constant load of Ty1 (Hénault et al., 2020). Our new whole-genome assemblies revealed that this apparent CN stability hides major variation in terms of Ty1 insertion position, which explains how LOH events can drastically alter the Ty1 landscape in CC crosses.

The contrasted mobilization dynamics suggest that intraspecific variation affects not only the potency of Ty1 transposition, but also the strength of its regulation. SpB genomes generally have low Ty1 CNs, potent Ty1 sequence variants, and a steep negative relationship of Ty1 transposition rate as a function of standing genomic Ty1 CN. This latter pattern is entirely consistent with the CNC mechanism identified for Ty1 in S. cerevisiae, although the functional demonstration of CNC in our system remains to be done. On the other hand, the Ty1 load is higher in SpC genomes, but SpC Ty1 variants and mtDNAs are linked to markedly lower transposition rates. Still, our data suggest that transposition rates in SpC are much higher than what would be predicted by extrapolating the steep CNC-like negative relationship of SpB genomes (Figure 5). The less potent SpC Ty1 variant seems to have established a state that can maintain substantial polymorphism in the population, perhaps enabled by lower or non-functional CNC regulation. The first alternative start codon from which the p22 protein can be expressed in S. cerevisiae is conserved in SpB and SpC Ty1 variants (M249, orthologous to M253 in the S. cerevisiae Gag protein sequence alignment from Czaja et al., 2020), suggesting that both could express p22. Despite recent structural insights into the mechanism of p22-mediated CNC (Cottee et al., 2021), the molecular basis of the difference between CNC+ and CNC- p22 variants in S. cerevisiae remains unclear (Czaja et al., 2020). Variation outside of p22 is also likely to impact CNC. For instance, the expression of CNC components in S. cerevisiae is affected by the Mediator transcriptional regulator complex (Salinero et al., 2018), providing the host genome with a mechanism to modulate the action of CNC (Curcio, 2019). We can hypothesize that intraspecific differences in Ty1 mobilization rate may stem from complex interactions between Ty1 potency, Ty1 self-regulation and host regulation.

In conclusion, our work provides a detailed account of the evolution of TE landscapes in the genomes of hybrid yeast MA lines, through an accurate resolution of their subgenomes by phased assembly. We highlight that retrotransposition is only a minor contributor to changes in TE load compared to transposition-unrelated SVs. While transposition rate harbors substantial variation that is underpinned by multiple sources of genetic diversity at the intraspecific level, its potential for shaping the TE landscape in Saccharomyces hybrids is likely to be overpowered by other forces of structural genome evolution.

Material and methods

Experimental evolution by MA and long-read sequencing

The MA evolution experiments used in this study were described previously (Charron et al., 2019; Hénault et al., 2020). Briefly, crosses were made between 13 haploid derivatives of wild yeast isolates (11 S. paradoxus isolates and two S. cerevisiae isolates). A total of 11 crosses were designed to span a range of evolutionary divergence between the parental strains, from very low divergence at the intraspecific level to very high divergence at the interspecific level. Each cross was replicated between 48 and 96 times with independent matings. The resulting collection was evolved under an MA regime for approximately 770 mitotic generations by streaking for single colonies on YPD agar medium (Supplemental Table S9) at 3-day intervals. Glycerol stock archives were made of the entire collection at regular intervals, and a subset of 10-12 MA lines per cross were randomly selected for long-read sequencing. Long-read library preparation and sequencing were described in (Hénault et al., 2022). Briefly, DNA was extracted using a standard phenol-chloroform protocol. Native (PCR-free) genomic DNA libraries were prepared using the SQK-LSK109 kit (Oxford Nanopore Technologies [ONT]) and multiplexed with the EXP-NBD104 barcoding kit (ONT). Sequencing was performed on a MinION sequencer (MIN-101B, ONT) with FLOMIN106 (revC) flowcells. The sequencing was run using MinKNOW v3.3.2 (ONT) and the basecalling was run using guppy v3.0.3 (ONT). Demultiplexing was done using guppy_basecaller v3.1.5.

Haploid genome assembly and annotation of the parental strains

De novo haploid genome assemblies for the 13 parental strains of the MA crosses were produced from basecalled long reads using wtdbg2 v2.5 (Ruan and Li, 2020) with parameters –p 0 –k 15 – AS 2 –s 0.05 –L 8000 –g 12m –X 120. Basecalled long reads were aligned to the draft assemblies using Minimap2 v2.17 (Li, 2018) with preset map–ont. Draft assemblies were polished with the long-read alignments and the linked raw nanopore signal data using nanopolish v0.13.3 (Loman et al., 2015) with parameter ––min–candidate–frequency 0.1. Short-read libraries from the corresponding 13 parental strains were retrieved from NCBI (accession number PRJNA515073). Reads were trimmed using Trimmomatic v0.33 (Bolger et al., 2014) with parameters ILLUMINACLIP:{custom adapters file}:6:20:10 MINLEN:40. The short-read libraries were mapped on the signal-level polished draft assemblies using BWA-MEM v0.7.17 (Li, 2013) and SAMtools v1.15 (Li et al., 2009). The signal-level polished draft assemblies were polished with the short- read alignments using Pilon v1.22 (Walker et al., 2014).

Polished de novo genome assemblies were scaffolded at the chromosome level using the following procedure. Reference genomes for S. paradoxus strains LL2012_001 (SpA), MSH-604 (SpB) and LL2011_012 (SpC) (Eberlein et al., 2019) were scaffolded using RaGOO v1.1 (Alonge et al., 2019), using the genome of S. cerevisiae strain S288c (Yue et al., 2017) as a reference. For each of the MA parental strains, polished contigs were aligned against the scaffolded reference of the corresponding species or population using the nucmer (with preset ––mum) and show-coords (with parameters –r –d –T) tools from the MUMmer v3.23 suite (Kurtz et al., 2004). Contigs were sorted and merged into chromosomes using a custom Python v3.9.10 (Van Rossum and Drake, 2009) script. We manually corrected for misassemblies that would yield dicentric or acentric chromosomes, or that were incompatible with karyotypes determined by CHEF-PFGE (Charron et al., 2014b). Chromosome-level assemblies were masked for rDNA repeats as follows. Repeats from the S288c reference genome (release R64-2-1, downloaded from were extracted from chromosome XII (NC_001144, 451417-468931) and searched against the chromosome-level assemblies using BLASTN v2.11.0 (Camacho et al., 2009). Sequences corresponding to hits with alignment lengths over 200 bp were hard-masked using a custom Python v3.9.10 script.

Final assemblies were annotated for families of Ty LTR retrotransposons using RepeatMasker v4.1.2-p1 (Smit et al., 2015) with parameters –s –nolow –no_is –gccalc and a custom database of reference Ty sequences from S. paradoxus (lib_Sp.fasta) or S. paradoxus and S. cerevisiae combined (lib.fasta). RepeatMasker outputs were defragmented using REannotate version 26.11.2007 (Pereira, 2008) with parameters -s 15000 -d 10000. Final assemblies for the two parents of each MA cross were aligned to each other using the nucmer (with preset ––mum) and show-coords (with parameters –r –d –T) tools from the MUMmer v3.23 suite. The orthology between the full-length Ty annotations for each cross was scored from visual inspection of the alignments.

Sorting of reads per MA line subgenome

For each of the 11 crosses of the MA experiment, genomic variants that distinguish the two parental genomes were obtained from a variant call dataset previously generated (Hénault et al., 2020) from short reads mapped on the reference genome of S. paradoxus SpB strain MSH-604 (Eberlein et al., 2019). Informative variants were identified with bcftools v1.16 (Li, 2011) using a custom bash script. Long-read libraries were mapped against the MSH-604 genome (Eberlein et al., 2019) and a custom Python v3.9.10 script was used to classify individual reads as one parental genotype or the other. Each read had to have at least two informative variants. For a read to be classified as originating from the subgenome of one parent, it had to be supported by a count of informative variants at least twice as high as the count of informative variants supporting the opposing parental subgenome. Lists of read IDs for reads successfully classified were used to split MA lines libraries using Seqtk v1.3-r106 (https ://

Subgenome-level assembly and annotation of MA line subgenomes

Basecalled long reads split by subgenome for each MA line were assembled separately using wtdbg2 v2.5 with parameters –p 0 –k 15 –AS 2 –s 0.05 –g 12m. Draft assemblies were polished with basecalled reads using medaka v1.4.4 ( with model r941_min_high_g303. Polished assemblies were annotated for families of Ty LTR retrotransposons using the same methodology as for haploid parental genomes (see above). To enable the comparison of Ty annotations between the different assemblies, the following procedure was designed to establish a common coordinate system and to define orthology relationships. Subgenome-level assemblies were aligned against the corresponding haploid parental genome using Minimap2 v2.20-r1061 with preset –x asm10. Ty annotation coordinates were exported into BED format using a custom Python v3.9.10 script and lifted over to haploid parental genome coordinates using paftools liftover v2.20-r1061 (Li, 2018). The resulting annotation coordinates were merged in clusters (roughly corresponding to Ty orthogroups) in a custom Python v3.9.10 script using the DBSCAN algorithm from the scikit-learn v1.1.3 Python package (Pedregosa et al., 2011) with parameters eps=500 min_samples=1. Annotations within one cluster were required to share the same Ty family and strand orientation. Clusters were classified as complex or non-complex, depending on whether they contained more than one annotation in any genome.

Classification of orthologous Ty clusters

For each cluster of orthologous Ty annotations in each MA line subgenome assembly, we assigned an SV class, if applicable. Unless otherwise stated, all the steps of the classification procedure were performed using custom Python v3.9.10 scripts.

Basecalled long reads split by subgenome were mapped against the corresponding parental assembly using Minimap2 v2.20-r1061 with preset map–ont, and alignments were filtered using SAMtools v1.16.1 to remove secondary alignments. Depth of coverage values from these alignments were extracted using SAMtools v1.16.1. Tracts of discrete relative depth of coverage along each genome were defined as follows. Assemblies were subdivided into 30 kb-wide non- overlapping windows, and the median depth of coverage value of each window was divided by the genome-wide median. Adjacent windows having the same value were merged to generate tracts that reflect major CN changes along the genome (i.e. polyploidy, aneuploidies and LOH events).

In addition to the discrete estimates of relative depth of coverage along each subgenome, the existence of a subgenome-level assembly was inferred for each parental Ty cluster using a reverse liftover procedure as follows. The parental genomes were aligned against the corresponding subgenome-level assemblies using Minimap2 v2.20-r1061 with preset –x asm10. For each Ty cluster, the two 500 bp-wide windows immediately flanking the annotation were exported into BED format and lifted over to subgenome-level assembly coordinates using paftools liftover v2.20-r1061. For the downstream steps, the clusters having inconsistent depth of coverage-based CN estimates and assembly alignment-based presence (i.e. having a CN of zero and assembly presence, or a CN greater than zero and assembly absence) were excluded.

These data were organized as boolean variables as follows. At the level of the individual orthologous cluster, the variables included were: whether the cluster is complex (comprising more than a single annotation per genome); and whether the cluster contains an annotation in the corresponding parental genome. At the level of the individual orthologous cluster in individual MA line subgenomes, the variables included were: whether the cluster contains an annotation; whether there is the presence of an assembly based on assembly alignment; whether the content of the cluster is the same as the parent; and whether the CN of the cluster is the same as the parent. The CN of the cluster and the types of annotations in the cluster are non-boolean variables that were also included at this level.

Using these variables, a decision tree (Supplemental Figure S5) was used to attribute one of the following SV classes for each orthologous cluster in each MA line subgenome. The majority of clusters were identical to the corresponding cluster in the parental genome, and were called “No change”. The clusters which had identical contents to the parental genome, but in a different CN, were called “Polyploidy/aneuploidy/LOH”. The clusters which had a full-length annotation in the parent and a truncated annotation in the MA line subgenome were called “Truncation”. The clusters which had a full-length annotation in the parent and a solo LTR in the MA line subgenome were called “Excision”. The clusters which had a unique additional full-length annotation in the MA line subgenome compared to the parent were called “De novo”. The clusters which had an annotation in the parental genome, but no annotation in the MA line subgenome despite having support for MA line subgenome assembly presence, were called “Deletion”.

We manually classified loci from the class “Polyploidy/aneuploidy/LOH” using the mappings of long read libraries against parental assemblies. Genome-wide relative depth of coverage profiles were generated by binning values in non-overlapping windows of 10 kb. Profiles were individually examined to determine if CN changes were due to polyploidy, aneuploidy or large tracts of LOH.

Identification of de novo transposition events

From the classification produced by the decision tree, the loci identified as “De novo” were curated to identify confident loci corresponding to retrotransposition events. We extracted the sequences immediately flanking the insertion loci and looked for short repeated motifs in direct orientation, corresponding to TSDs. TSDs of five or six nucleotides were found, and loci with no identifiable TSD were discarded. We produced chromosome alignments comprising the MA line subgenome(s) with de novo loci and all parental strains using Mauve v2015-02-26 (Darling et al., 2010) with the progressiveMauve algorithm and default parameters. Alignments were inspected to confirm that the junctions of de novo loci were unique to the corresponding MA line subgenome. Finally, we compared the TSD motifs of candidate de novo retrotransposition to TSD motifs of all parental full-length copies to filter out non-unique ones. The remaining loci (all from the Ty1 family) were classified as de novo retrotransposition events.

A phylogenetic analysis of the de novo Ty1 insertions was performed by extracting the sequences of de novo loci and of all full-length Ty1 and Ty2 parental elements. We added the sequences of representative Ty1 and Ty2 elements identified by (Bleykasten-Grosshans et al., 2021) as references. We included the 50 bp flanking sequences in 5’ and 3’ of all extracted sequences and ran a multiple sequence alignment using MUSCLE v3.8.31 (Edgar, 2004). The alignment was trimmed manually using MEGA v11.0.11 (Tamura et al., 2021). A maximum likelihood phylogenetic tree was computed on the trimmed alignment using RAxML-NG v1.1.0 (Kozlov et al., 2019) with the GTR+G model initiated with 10 parsimony trees and 200 bootstraps. The phylogenetic tree was visualized using FigTree v1.4.4 ( Additionally, we built a phylogenetic network from the same trimmed alignment using SplitsTree v4.18.3 (Huson and Bryant, 2006) with default parameters.

Plasmids used and constructed in this study

Plasmids used and constructed in this study are listed in Supplemental Table S2. Oligonucleotide sequences are detailed in Supplemental Table S3. PCR reactions and cycles are detailed in Supplemental Table S4. Media recipes are detailed in Supplemental Table S9. All the PCRs used for cloning were performed using the KAPA HiFi HotStart DNA polymerase (Roche). All the PCRs used for cloning confirmations were performed using the Taq DNA Polymerase (BioShop). Plasmid minipreps were performed on overnight cultures in 5 mL 2YT+Amp or 2YT+Kan medium, depending on the plasmid, using the Presto Mini Plasmid Kit (GeneAid).

Gibson cloning reactions were performed as follows. A Gibson assembly master mix was prepared with the following components (for 1.2 mL): 360 μL of 5X ISO buffer (500 mM Tris-Cl, 50 mM MgCl2, 4 mM dNTPs mix, 50 mM DTT, 250 mg mL-1 PEG 8000, 5 mM NAD+), 0.64 μL 10 U μL-1 T5 exonuclease (New England Biolabs), 20 of 2 U µL-1 Phusion High-Fidelity DNA Polymerase (New England Biolabs), 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs) and 700 µL of PCR-grade H2O. Gibson reactions were assembled by adding all the components to 7.5 µL of Gibson master mix, and completing the volume to 10 µL with PCR-grade H2O. Gibson reactions were incubated at 50°C for 60 min.

Competent cells of Escherichia coli were prepared as detailed in Green and Rogers (Green and Rogers, 2013). E. coli transformations were performed by combining 100 µL of competent cells with 5 µL of mutagenesis or Gibson assembly reaction and incubating the mixture on ice for 15 min. Heat shock was done at 42°C for 1 min, followed by incubation on ice for 5 min. 900 µL of 2YT medium was added and the mixture was incubated for 45 min at 37°C with agitation. Cells were plated on 2YT+Amp agar or 2YT+Kan agar medium, depending on the plasmid, and incubated at 37°C for 24 hrs.

Construction of Ty1 retrotransposition marker plasmids

Retrotransposition rate measurement assays were developed based on the plasmid-borne assay designed by Curcio and Garfinkel (Curcio and Garfinkel, 1991) with several modifications. First, we replaced the S. cerevisiae Ty1 sequence with variants sampled from wild S. paradoxus genomes. To measure transposition rates at a transcription rate closer to the natural genomic context and prevent potential biases arising from galactose induction in multiple wild genetic backgrounds, we opted for the native promoter of Ty1 variants instead of the GAL1 promoter. Finally, we opted for a low CN centromeric plasmid backbone instead of a high CN plasmid backbone in order to increase the mitotic stability and reduce the CN variance of the system.

Plasmid pRS31N was constructed as follows. Using a pFA6-natNT2 (Janke et al., 2004) miniprep as a template, we amplified the natNT2 resistance cassette against nourseothricin (Nat). Using a pRS316 (Sikorski and Hieter, 1989) miniprep as a template, we amplified the backbone excluding the URA3 selection marker. A Gibson assembly mix was prepared by adding 1 µL of natNT2 cassette and 1 µL of backbone to the Gibson master mix. After incubation for 60 min at 50°C, the Gibson reaction was digested with DpnI (New England Biolabs). Competent cells of E. coli MC1061 (Casadaban and Cohen, 1980) were transformed with 5 µL of the Gibson reaction. Cloning was confirmed by PCR by testing the 5’ junction. A miniprep was migrated by agarose gel electrophoresis and transformed into yeast, selecting on both YPD+Nat agar medium and SC- ura agar medium.

Plasmids pRS31N-Ty1-SpB-HIS3AI and pRS31N-Ty1-SpC-HIS3AI were constructed as follows. The plasmid pGTy1-H3HIS3AI was a gift from David Garfinkel (Addgene plasmid # 62228 ; ; RRID:Addgene_62228) (Curcio and Garfinkel, 1991). Using a pGTy1-H3mHIS3AI miniprep as a template, we amplified the retrotransposition indicator gene (RIG) HIS3AI. We treated the HIS3AI RIG amplicon with DpnI followed by purification using solid- phase reversible immobilization (SPRI) beads. We digested the plasmid pRS31N with SacII (New England Biolabs), treated the digestion with calf intestinal alkaline phosphatase (CIP, New England Biolabs) and purified the digested plasmid using SPRI beads. We extracted genomic DNA from two S. paradoxus wild diploid strains (MSH-604, SpB; and LL2011_012, SpC) by following a standard phenol-chloroform extraction protocol on overnight cultures in 5 mL YPD medium incubated at 30°C with agitation. A genomic region of ∼10 kb containing a single full- length Ty1 element was amplified from the MSH-604 (chrXV) and LL2011_012 (chrVII) genomic DNA extractions using unique primers. The full-length element chosen from the LL2011_012 genome was identical to five other full-length Ty1 elements in the same genome, and one of the two full-length Ty1 elements in MSH-604 was chosen at random. From each genomic region amplicon, the full-length Ty1 element was amplified in two segments: a first segment spanning the left LTR and complete internal ORF (Ty1 5’ segment), and a second segment spanning the right LTR (Ty1 3’ segment). The Ty1 segment amplicons were purified with SPRI beads. A first Gibson assembly reaction was performed by combining 40 fmol each of the digested pRS31N backbone, the Ty1 5’ segment amplicon and the HIS3AI RIG amplicon. Competent cells of Escherichia coli MC1061 were transformed with 5 µL of the Gibson assembly reaction. Cloning was confirmed by PCR by testing the 5’ and 3’ junctions. We prepared a miniprep of the first Gibson assembly and validated its restriction profile with EcoRV (New England Biolabs). The miniprep of the first Gibson assembly was digested with NotI (New England Biolabs), and the digestion was treated with CIP and purified using SPRI beads. A second Gibson assembly reaction was performed by combining 40 fmol each of the first Gibson assembly digestion and the Ty1 3’ segment amplicon. Cloning was confirmed by PCR by testing the 5’ and 3’ junctions. We prepared a miniprep of the second Gibson assembly and validated its restriction profile with EcoRV. We confirmed the final clones with long-read whole-plasmid sequencing at Plasmidsaurus (

Yeast strains used and constructed in this study

S. paradoxus strains used and constructed in this study are listed in Supplemental Table S5. Oligonucleotide sequences are detailed in Supplemental Table S3. PCR reactions and cycles are detailed in Supplemental Table S4. Media recipes are detailed in Supplemental Table S9. All the PCRs for plasmid mutagenesis and cassette amplification were performed using the KAPA HiFi HotStart DNA polymerase. All the PCRs used for confirmations or diagnostics were performed using the Taq DNA Polymerase.

S. paradoxus competent cells were prepared from 25 mL cultures in YPD medium incubated at 30°C with agitation until they reached an optical density at 600 nm (OD600 mL-1) of 0.5-0.7. Cultures were centrifuged at 500✕g for 5 min. Cells were washed once in 5 mL in H2O and once in 5 mL SORB solution (1 M sorbitol, 100 mM lithium acetate, 10 mM Tris-Cl, 1 mM EDTA). Cells were resuspended in 180 µL of SORB solution and 20 µL of salmon sperm DNA that was previously boiled for 5 min and cooled on ice. Competent cells were stored at -70°C until use.

S. paradoxus transformations were performed by combining 20 µL of competent cells, 250 ng of plasmid or 8 µL cassette amplicon, and 100 µL of Plate Mixture solution (40% PEG 3350, 100 mM lithium acetate, 10 mM Tris-Cl, 1 mM EDTA). The mixture was incubated at room temperature for 30 min. 7.5 µL of DMSO were added and a heat shock was performed at 37°C for 30 min. Cells were centrifuged at 2000 rpm for 3 min and resuspended in 100 µL of YPD medium for 2 hrs in the case of plasmid transformations, or 5 hrs in all other cases.

Generation of histidine auxotroph strains

Short placeholder sequences (hereafter referred to as stuffers) (Dionne et al., 2021) to be used as donor DNA in CRISPR-Cas9 mediated deletion of HIS3 (YOR202W) were amplified using the following reactions: 2 µL of Taq 10X Buffer (BioShop), 1.2 µL of 25 mM MgCl2, 0.4 µL of 10 mM dNTPs mix, 0.4 µL of 10 µM 5’ primer, 0.4 µL of 10 µM 3’ primer, 2 µL of 0.01 µM template primer, 0.12 µL of Taq DNA Polymerase and 13.5 µL of PCR-grade H2O. The following PCR cycle was run: 3 min at 94°C; 35 cycles of 30 sec at 94°C, 30 sec at 68°C, and 15s at 72°C; 25 sec at 72°C; hold at 10°C.

A plasmid encoding CRISPR-Cas9 and a guide RNA (gRNA) sequence targeting the HIS3 coding sequence in S. paradoxus was designed as follows. The plasmid pCAS was a gift from Jamie Cate (Addgene plasmid # 60847 ; ; RRID:Addgene_60847) (Ryan et al., 2014). pCAS was used as a template to mutagenize the gRNA sequence to target the coding sequence of the PEX11 gene (YOL147C). Mutagenesis PCRs were treated with DpnI and transformed in E. coli MC1061. The mutagenesis was confirmed by Sanger sequencing of the gRNA locus from a miniprep. pCAS-PEX11 was used as a template to mutagenize the gRNA sequence to target the coding sequence of HIS3. Mutagenesis PCRs were treated with DpnI and transformed in E. coli MC1061. The gRNA locus was amplified for confirmation, and the mutagenesis was confirmed by digesting the amplicon with HinfI (New England Biolabs) (the PEX11 gRNA sequence contains a HinfI restriction site, while the HIS3 gRNA doesn’t) and by Sanger sequencing.

CRISPR-Cas9 mediated deletion of HIS3 was done following the method of Ryan et al. (Ryan et al., 2016) with pCAS-HIS3 and the corresponding stuffer amplicon in eight different haploid S. paradoxus strains derived from wild isolates (Charron et al., 2014b; Leducq et al., 2016). Gene deletion was confirmed by PCR and growth on SC-his agar medium.

To enable crosses among three of the his3::stuffer strains, one per population (SpA: YPS744, SpB: UWOPS-79-140, SpC: LL2011_012), the hphMX resistance cassette against hygromycin B (HygB) at the HO locus was replaced by the kanMX resistance cassette against geneticin (G418), and the mating types were switched.

The plasmid pUG6 (Güldener et al., 1996) was obtained from Euroscarf ( pUG6 was used as a template to amplify the kanMX cassette with flanking homology to the HO locus. Competent cells were transformed with the cassette and plated on YPD+G418 agar medium. Cassette switch was confirmed by PCR.

The plasmid pHS3 was a gift from John McCusker (Addgene plasmid # 81038 ; ; RRID:Addgene_81038). Competent cells from the mating type switched strains were transformed with pHS3 and plated on YPD+G418+Nat agar medium. Colonies were grown in 3 mL YPD medium at room temperature with agitation for two days to allow for pHS3 segregation, diluting cultures ∼1000X after 24 hrs. Cultures were streaked on YPD plates and incubated at room temperature for five days. Single colonies were checked for the loss of pHS3 by making patches on YPD+Nat agar medium and for autodiploidization by PCR (Huxley et al., 1990). Strains were sporulated as described in Plante and Landry (Plante and Landry, 2020) and tetrads were dissected on YPD agar medium using a SporePlay microscope (Singer Instruments). Dissection plates were incubated at room temperature for five days. The mating types of spores were confirmed by PCR (Huxley et al., 1990).

To enable crosses with reciprocal mtDNA inheritance, we generated strain derivatives devoid of mtDNA (ρ0) as described in Fox et al. (Fox et al., 1991). The loss of respiration ability was confirmed by spotting the strains on YPEG agar medium.

Crosses with reciprocal mtDNA inheritance

Histidine auxotroph strains and their HO cassette-switch, mating type-switch and ρ0 derivatives were crossed as detailed in Supplemental Table S6. For each parental strain, an overnight preculture was grown in 1 mL of YPD medium at room temperature. 100 µL of each preculture were combined in 1 mL YPD medium and incubated at room temperature without agitation for 4 hrs. 10 µL of mating cultures were streaked on YPD+G418+HygB agar medium and incubated for three days at room temperature. Two single colonies per streaking were used to inoculate 1 mL of YPD medium and the strains were tested for respiratory proficiency by spotting on YPEG agar medium. The strains were also tested for diploidy by cellular DNA staining and fluorescence measurement by flow cytometry using a Guava easyCyte 8HT flow cytometer (EMD Millipore) as described in Marsit et al. (Marsit et al., 2021).

Measurement of retrotransposition rates

Fluctuation assays were performed to measure retrotransposition rates. Competent cells of the corresponding haploid strains and diploid crosses were transformed with minipreps of either pRS31N-Ty1-SpB-HIS3AI or pRS31N-Ty1-SpC-HIS3AI, and plated on YPD+Nat agar medium. Transformation plates were incubated at 30°C for three days. From the transformation plates, single colonies were picked and resuspended in 10 mL of YPD medium. Cell densities were estimated by measuring the OD600 of the resuspensions, and dilutions at 2✕10-3 OD600 mL-1 were prepared in YPD medium. 96-well plates were filled with 225 µL of YPD+Nat 1.1X medium. 25 µL of cell dilutions were added to each well for a final concentration of 2✕10-4 OD600 mL-1 (∼500 cells per well). Each combination of background and Ty1 variant was replicated in 48 independent cultures (half a plate). Plates were covered with breathable rayon films and incubated at 20°C for three days. Final cultures were centrifuged at 500✕g for 5 min and resuspended in 200 µL of H2O. The entire resuspensions were spotted in groups of 7 or 9 on SC-his agar plates that were previously dried at 37°C for three days. Plates were incubated at room temperature and His+ colonies were counted after five days.

For each haploid background, 42 cultures were spotted on SC-his agar plates and six were pooled. Serial dilutions were prepared by diluting pools twice 200X and 100 µL of final dilutions were plated on YPD agar plates. Plates were incubated at room temperature for 3 days and colonies were counted to estimate final population sizes. Colony counts are detailed in Supplemental Table S7. For each diploid background, 36 cultures were spotted on SC-his agar plates and 12 were pooled. Serial dilutions were prepared by diluting pools twice 12X and cell densities of the final dilutions were measured by flow cytometry using a Guava easyCyte 8HT flow cytometer to estimate final population sizes. Colony counts are detailed in Supplemental Table S8.

The number of mutations (retrotransposition events) in each culture and associated 95% confidence intervals were estimated using the newtonLD and confintLD functions from the Python implementation of the R package rSalvador (Zheng, 2017) ( rSalvador/blob/master/pysalvador/ Retrotransposition rates were computed by dividing mutation count estimates by the estimates of final population sizes.

Data accessibility

Nanopore long-read sequencing data (basecalled reads) and parental genome assemblies are available at NCBI under accession number PRJNA828354. Illumina short-read sequencing data of the MA lines is available at NCBI under accession number PRJNA515073. Custom scripts, custom databases of reference Ty sequences and subgenome-level assemblies of MA lines used in this study are available at GitHub (

Competing interests

We declare no competing interest.


We thank Alexandre Dubé for helpful comments on the manuscript and contributions to plasmid constructions. We thank Anna Fijarczyk and Hélène Martin for contributions to sequencing data preprocessing. We thank the members of the Landry laboratory for helpful discussions on the project. This project was supported by funding to C.R.L. from an NSERC discovery grant (RGPIN- 2020-04844) and an FRQNT team grant (2019-PR-254415), and an NSERC Alexander Graham Bell doctoral scholarship to M.H. C.R.L. holds the Canada Research Chair in Cellular Systems and Synthetic Biology.

Author contributions

C.R.L. and M.H. designed the project. M.H. performed the analyses and experiments. M.H., S.M., and G.C. performed the MA evolution experiment and short-read sequencing as cited. M.H. wrote the manuscript with input from all authors.