Association of modules to experimental variables.
a-g, Bubble plots showing statistical association between MEG expression and variables of interest in various sample subsets. Strength of association (r²) is encoded by bubble size, significance (p) is encoded in the color scale with significant associations darkly bordered. Pearson correlation and Students t test, alpha=0.05. Plots show the associations between gene modules (rows) to; a-b, vehical treated song system specializations, comparing MEG expression in song system samples from either sex to their appropriate surrounding controls; c-d, E2-treated song system specialization, same comparison as a-b but within E2-treated samples; e, female vocal learning capacity after E2, comparing E2 treated female song system components to all other female samples from that circuit node; f, sexual dimorphism within the song system, comparing vehicle treated male and female song system components; g, sexual dimorphism within the surrounding control regions, comparing the vehicle treated male and female surrounding control samples. Each neural circuit node is considered separately (columns). h-k, Expression of modules with strong region specific expression. Module A is highly expressed in Str and Area X samples, with addtional differences between RA and LAI (h); Module C is highly expressed in the arcopallium, especially RA, with some increaase in HVC (i); Module F is expressed highly only in LMAN regardless of sex or treatement (j); Module G is only highly expressed in HVC, where it differs both by sex and treatment (k).