Col11a1 regulation of Mmp3 expression in cartilage.
a) PCR assay of Col11a1 excision in Col11a1fl/fl cultured rib cartilage cells.
b) Gene expression levels from Col11a1fl/fl cultured rib cartilage cells transduced with GFP (Ad5-GFP, left) or Cre expressing adenovirus (Ad5-cre, right) as determined by qRT PCR. Each value represents the ratio of each gene expression to that of GAPDH, and values are mean ± standard deviation. The expression value of control Ad5-GFP results (N≥ 4 experiments) was arbitrarily set at 1.0. Statistical differences were determined using a two-sided unpaired t test (P*<0.05).
c) Representative Western blot of cultured cartilage cells after Ad5-GFP or Ad5-cre transduction. Protein size ladder is shown in lane 1. Quantification of bands detected by Western blotting, where scramble results were set to 1.0 from N=4 independent experiments is shown at right.
d) Gene expression levels from dissected Col11a1fl/fl ATC rib cartilage, analyzed as described in a). Results shown are from N=3 mice.