Isobaric contractile Assessment of popliteal cLV from PdgfrαCreERTM driven deletion of Ano1, CX45, and CaV1.2
Summary of the contractile parameters recorded from popliteal cLVs in PdgfrαCreERTM-Ano1fl/fl, PdgfrαCreERTM-Cx45fl/fl mice, PdgfrαCreERTM-Cav1.2fl/fl mice. Contraction frequency (A, D, G), ejection fraction (B, E, H), and vessel tone (C, F, I) were assessed. No statically significant differences observed in cLVs isolated from PdgfrαCreERTM-Ano1fl/fl and PdgfrαCreERTM-Cx45fl/fl mice across these three parameters. Mean and SEM shown, n=6 popliteal vessels from 3 mice PdgfrαCreERTM-Ano1fl/fl mice and n=10 popliteal vessels from 6 mice Ano1fl/fl mice. Mean and SEM shown, n=5 popliteal vessels from 3 mice PdgfrαCreERTM-CX45fl/fl mice and n=8 popliteal vessels from 11 mice CX45fl/fl mice. Mean and SEM shown, n=6 popliteal vessels from 3 mice PdgfrαCreERTM-Cav1.2fl/fl mice and n=9 popliteal vessels from 20 mice Cav1.2fl/fl mice. The contractile data from control Cav1.2fl/fl vessels was previously published but was separated by sex (Davis et al., 2022) while they are combined here. * Denotes significance at p <0.05 which 0.10 > p >0.05 are reported as text. Normalized contraction amplitude, fractional pump flow, end diastolic diameter can be found in Supp Figure 8.