Spatiotemporal expression of Bm-mamo.
(A) Temporal expression of Bm-mamo. In the molting stage and adult stage, this gene was significantly upregulated. M: molting stage, W: wandering stage, P: pupal stage, P1: Day 1 of the pupal stage, A: adult stage, 4th3d indicates the 4th instar on Day 3. 1st to 5th denote the first instar of larvae to fifth instar of larvae, respectively.
(B) Tissue-specific expression of 4th-instar molting larvae. Bm-mamo had relatively high expression levels in the midgut, head, and epidermis. AMSG: anterior division of silk gland and middle silk gland, PSG: posterior silk gland.
(C) Detailed analysis of Bm-mamo at the 4th larval stage in the epidermis of the Dazao strain. Bm-mamo expression is upregulated during the molting stage. HCS indicates the head capsule stage. The “h” indicates the hour, 90 h: at 90 hours of the 4th instar.