Suppression of vmPFC PV neurons in control animals.
(A) Speed of eYFP-expressing mice during avoidance with (red) and without (dark brown) illumination. (B) Distribution of crossing latencies during avoidance in eYFP-expressing animals with (red) and without illumination (brown). (C-D) Same as (A-B) for approach. (E) Freezing probabilities of NpHR-expressing animals during avoidance with (red) and without (dark brown) illumination. (F) Difference in freezing probabilities between with (on) and without (off) illumination in NpHR-expressing mice (red) and in eYFP- expression mice (grey). (G) Difference in freezing probabilities, averaged over the laser stimulation period, between with (on) and without (off) illumination in NpHR-expressing mice (red) and in eYFP- expression mice (grey). (H) Change in ratio of freezing duration/avoidance latency of NpHR-expressing and eYFP-expression mice during illumination compared to non-illumination. (I) Crossing latencies during avoidance in NpHR-expressing mice, grouped by relative position prior or within a stimulation block. (J) Average crossing latencies of all non-illuminated trials (off), 1st trials (1st stim) and 2nd-6th trials in illuminated blocks (2-6th stim). (K) Crossing latencies during avoidance in NpHR-expressing mice, grouped by relative position within or after a stimulation block. (L) Average crossing latencies of all illuminated trials (stim), 1st trials (1st off) and 2nd-6th trials (2-6th off) in non-illuminated blocks. (M) Post- crossing optogenetic inactivation schematic. (N) Speed of NpHR-expressing mice during avoidance with (red) and without (dark brown) illumination. (O) Same as (N), but the y-axis was plotted on a log scale. (P) Change in speed of NpHR-expressing and eYFP-expression mice during illumination (0 to 2 s from avoiding chamber crossing) compared to non-illumination trials. (Q) The latency of the first trials in stimulation blocks is compared to the latency of the second trials in stimulation blocks, and the latency of the first trials after stimulation blocks is compared to the latency of the second trials after stimulation blocks. n.s. p > 0.05, *p < 0.05, **p < 0.01. For (G) and (H), un-paired t-test. For (J), (L) and (Q), paired t- test. For (P), 2-way ANOVA interaction term. Shaded regions indicate SEM. NpHR: N=8 mice, eYFP: N=5 mice.