Expression profiles of FBXO24 during testicular development and spermatogenesis in mice.
(A) qPCR analysis of FBXO24 mRNA levels in multiple organs in mice. n = 3/group. Data are mean ± S.D. *, p < 0.05. (B) qPCR analysis of FBXO24 mRNA levels in developing testes at postnatal day 0 (P0), P3, P7, P14, P28, and P35. n = 3/group. Data are mean ± S.D. *, p < 0.05. (C) qPCR analysis of FBXO24 mRNA levels in isolated spermatogenic cell populations, including spermatogonia (SG), Sertoli cells (SE), spermatocytes (SC), round spermatids (RS), and elongating spermatids (ES). n = 3/group. Data are mean ± S.D. *, p < 0.05. (D) Western blot analysis of FBXO24-HA expression in the tissues of adult transgenic mice. (E-F) Co-immunostaining of FBXO24-HA (red) and (E) γH2AX (green) or (F) PNA (green) in adult Fbxo24-HA testes. Scale bar, 25 μm. DNA (blue) is stained by DAPI. Spermatogenic stages are noted.