The scarcity of hematopoietic stem cells (HSCs) restricts their use in both clinical settings and experimental research. Here, we examined a recently developed method for expanding rigorously purified murine HSCs ex vivo. After three weeks of culture, only 0.1% of cells exhibited the input HSC phenotype, but these accounted for almost all functional long-term HSC activity. Input HSCs displayed varying potential for ex vivo self-renewal, with alternative outcomes revealed by single cell multimodal RNA- and ATAC-seq profiling. While most HSC progeny offered only transient in vivo reconstitution, these cells efficiently rescued mice from lethal myeloablation. The amplification of functional HSC activity allowed for long-term multilineage engraftment in unconditioned hosts that associated with a return of HSCs to quiescence. Thereby, our findings identify several key considerations for ex vivo HSC expansion, with major implications also for assessment of normal HSC activity.
This study presents valuable data on how HSCs are expanded under PVA cultures. The functional evidence supporting the claims of the authors is solid, although an extended multi-omic data analysis could have strengthened the study. The work will be of interest to individuals within this HSC field.
Hematopoietic stem cells (HSCs) are the functional units in bone marrow transplantation (BMT) and gene therapy approaches via their ability for multilineage differentiation and long-term self-renewal1. However, restrictions in HSC numbers, the requirement of harsh host conditioning and challenges with how to genetically manipulate HSCs with retained function are barriers for BMT/gene therapy2. Due to the lack of reliable models for accurate assessment of human HSCs, mice continue to be the preferred experimental model for studying HSC biology. However, studying murine HSC biology also poses challenges due to the scarcity of HSCs.
Recently, culture conditions supporting efficient maintenance and expansion of murine HSCs were reported3. However, the expansion of functional in vivo HSC activity over a 28-day period (estimated to 236-899-fold) was not on par with the total cell expansion (>104-fold), demonstrating that large amounts of differentiated progeny were generated along with self-renewing HSC divisions3. Moreover, candidate HSCs (cHSCs) by phenotype represented only a minor portion of the cells at the culture end-point3, although assessment of HSC activity based on this might be obscured by previous observations that HSCs change some phenotypes in culture4. While in vitro differentiation complicates assessments of HSC expansion, it might also be beneficial. For example, while HSCs can support lifelong hematopoiesis after bone marrow transplantation (BMT), they have limited capacity to rescue the host from lethal conditioning5. To address this limitation, there is a requirement for progenitor cells that can efficiently supply the host with mature blood cells, such as erythrocytes and platelets6,7.
When transplanted, in vivo HSC activity is typically determined using both quantitative and qualitative parameters8. Most commonly, this is achieved by comparing to a known amount of competitor cells9. However, although the competitive repopulation assay (CRA) represents a mainstay in experimental HSC biology, it associates with shortcomings often neglected. First, while clinical BMT aims at transplanting large numbers of HSCs10,11, experimental murine BMT typically use limited HSC grafts, which can strongly influence on HSC function12. Secondly, the CRA is from a quantitative perspective rather blunt, with sigmoidal rather than linear characteristics9. This limits the boundaries of the effects that can be measured. Thirdly, the CRA assesses HSC activity based on overall hematopoietic chimerism, which might reflect poorly on the actual HSC activity given the vastly different turnover rates of different blood cells lineages13. At the same time, alternative assays such as limit dilution assays or single-cell transplantation assays are cumbersome, expensive and associated with ethical concerns. Newer developments in DNA barcoding technology have overcome many of these issues14.
Another concern with BMT is the use of aggressive host conditioning to permit engraftment. While eradicating malignant cells is an important part of treatment regimens for leukemic patients, the fates of HSCs in conditioned hosts versus native hematopoietic contexts are dramatically different15. But transplantation into unconditioned recipients is inefficient and requires large amounts of transplanted HSCs to saturate available niches16,17. To achieve even limited HSC chimerism in unconditioned hosts can have strong long-term therapeutic effects18 and many experimental approaches might benefit from avoiding host conditioning.
Here, we provide molecular and functional details on HSC differentiation and self-renewal following culture and define a set of parameters critical for in vivo assessment of HSC activity. Importantly, we find that HSC heterogeneity was a key influencing factor for HSC expansion even among rigorously purified cHSCs. This determinism has broad implications for experiments involving in vitro manipulation of HSCs, but also when assessing normal HSC function by BMT.
In vivo HSC activity is restricted to cells expressing high levels of EPCR
We first assessed the in vivo reconstituting activity of cHSCs with different phenotypes. The endothelial protein C receptor (EPCR or PROCR/CD201) is highly expressed on HSCs19, while CD41 (ITGA2B) was suggested to be functionally relevant for HSC maintenance and homeostasis20. Based on expression of EPCR and CD41, we isolated 4 subfractions within BM SLAM LSK cells (Lin-Sca1+cKit+CD150+CD48-/low) (Fig 1A) and competitively transplanted them at equal numbers into lethally irradiated recipients (Fig 1B). The reconstitution from the three EPCRhigh fractions was restricted to the PB myeloid lineages 4-week post-transplantation (Fig 1C), and most of these recipients displayed robust long-term (beyond 16 weeks) multilineage reconstitution with equivalent cHSC chimerism in the BM (Fig 1C-D). EPCRhighCD41- cells produced slightly more lymphoid cells than the EPCRhighCD41+ cells at the endpoint (Fig S1), but the cHSC chimerism in the BM was very similar to other tested EPCRhigh fractions (Fig 1D). In contrast to Gekas et al.20, we failed to observe that staining with the CD41 antibody (MWreg30 clone) compromises HSC engraftment. Transplantation of EPCR negative cells failed to associate with either long-term PB or BM cHSC chimerism, demonstrating lack of HSC activity within this fraction (Fig 1C-D).
Next, single EPCR+ SLAM LSKs were index sorted (Fig S1B) and expanded ex vivo in an F12-Polyvinyl alcohol (PVA) based culture media21. At an early stage (8 days, Fig S1C), cultures initiated with SLAM LSK cells with higher EPCR expression proliferated less compared to those with lower EPCR expression. However, after longer expansion (25 days, Fig S1C), the EPCR higher cells generated on average a larger amount of progeny. To evaluate the HSC activity following ex vivo expansion and to correlate this directly to EPCR expression levels, the expanded cells from index sorted cultures of cHSCs were collected and transplanted into individual lethally irradiated mice (Fig S1D). While freshly and stringently isolated HSCs normally fail to rescue mice from lethal irradiation5, we here entertained that culturing of HSCs in addition to inducing self-renewal, would also generate progenitors that could radioprotect the hosts. If so, survival could be used as a proxy for combined hematopoietic stem and progenitor cell (HSPC) activity. Indeed, mice receiving progeny from SLAM LSK EPCR higher cells were more efficiently radioprotected (Fig 1E). Thereafter, we initiated cultures with EPCRhigh or EPCRlower SLAM LSK cells fractions (Fig S1E). Mice transplanted with cells expanded from EPCRhigh cells radioprotected 5/5 recipients, while only two out of 5 animals receiving the progeny of EPCRlow cells survived long-term. As expected, these surviving mice displayed exclusive test-cell derived myelopoiesis long-term after transplantation (Fig 1F).
Taken together, these experiments established that HSC activity was restricted to EPCRhigh HSCs, and such cells could effectively generate progeny in vitro that rescue mice from lethal irradiation. Therefore, we operationally defined our input cHSCs by their EPCRhigh SLAM phenotype.
Phenotypic heterogeneity from ex vivo expanded cHSCs
The PVA-dependent cell culture system was reported to efficiently support HSC activity ex vivo over several weeks3. However, the functional HSC frequency did not appear on par with the total amount of cells generated by the end of the culture period, suggesting the generation also of many differentiated cells3. To detail this, we expanded aliquots of cHSCs ex vivo for 14-21 days, followed by multicolor phenotyping. From 50 cHSCs, an average of 0.5 million (14 days) and 13.6 million cells (21 days) were generated (Fig S2A) and which associated with a phenotypic heterogeneity that increased over time (Fig S2B). With the assumption that HSCs might retain their cell surface phenotype in cultures, we quantified EPCRhigh SLAM cells and observed a decrease in their frequency over time, although this was numerically counteracted by the overall proliferation in cultures (Fig 2A). Thus, quantification of cHSCs translated into an average expansion of 54-fold (26- to 85-fold) and 291-fold (86- to 1,273-fold) at 14 and 21 days, respectively (Fig 2A).
To gain a deeper understanding of the cells generated in our ex vivo cultures, we collected cells from the entire culture and isolated nuclei for single cell multiome sequencing using a commercial platform from 10x Genomics. This approach combines single-cell RNA sequencing and single-cell ATAC sequencing, allowing for integrated analysis of these modalities. Data was analyzed in R22 using mainly the Seurat23 and Signac24 packages, with the SAILERX package25.
Based on the transcriptomic signatures and distal motif identities, we were able to identify not only early hematopoietic stem and progenitor cells (HSPCs), but also many differentiated myeloid cells in the expansion cultures (Fig 2B), however with little evidence for lymphoid differentiation. Assessment of a condensed HSC signatures (Fig S2C) confirmed that the cHSCs were primarily located to cluster 5 (Fig 2D), with trajectory analyses suggesting multiple differentiation pathways from early HSPCs to differentiated myeloid progeny (Fig 2C).
Since the EPCR expression also condensed to cluster 5 (Fig S2D), we further investigated whether the expression of EPCR marks cHSCs ex vivo by collecting ex vivo expanded EPCR+ cells for single-cell multiome sequencing. These cells displayed less heterogeneity than what we observed for the total culture (Fig 2B), which included a more even distribution of the condensed HSC signature (Fig 2E) as well as the expression of EPCR (Fig S2E). Here, the most pronounced separator for the interrogated cells attributed to the cell cycle position (Fig 2F).
To explore if other markers could provide additional phenotypic information for genuine HSCs in cultures, we applied an Fgd5 reporter mouse model previously shown to selectively mark HSCs in situ26. While the Fgd5 reporter only marked a subset of cells in culture (Fig S2F), we noted heterogeneous expression of CD48 within the Fgd5 positive fraction (Fig S2F-G) and that we failed to clearly demarcate in our single-cell multimodal data.
Collectively, these results demonstrated robust expansion of cHSCs using the PVA-based culture system, albeit with additional parallel generation of a large number of more differentiated cells. While the identities of most of the cells in cultures could be clearly deduced by their combined gene expression and chromatin accessibility profiles, this approach failed to inform on the heterogeneous expression pattern of CD48 on cHSCs (Fig S2).
Functional HSC activity associates to a minor LSK SLAM EPCRhigh fraction within ex vivo cultures
Given our inability to distinctly identify cHSCs by their molecular profiles, we next explored the possibility to prospectively isolate HSCs from the cHSC cultures and instead rely on their functional ability to long-term repopulate lethally irradiated hosts for readout. cHSCs were isolated and cultured ex vivo for 21 days and the expanded cells were separated into two equal portions: one portion was kept unfractionated, while three subpopulations of CD150+LSKs: EPCRhighCD48-/low, EPCRhighCD48+ and EPCR- were sorted from the other portion. The fractions were competitively transplanted into lethally irradiated mice, such that each recipient received fractions equivalent to the expansion from 50 cHSCs (EE50) along with 500,000 competitor WBM cells (Fig 3A).
Stable and very high long-term multilineage reconstitution was observed in all recipients of unfractionated cultured cells. By contrast, test-cell derived reconstitution was not recovered from EPCR- cells (Fig 3B and Fig S3). EPCRhighCD48+ cells produced very high reconstitution levels short-term after transplantation, but the chimerism provided by these cells dropped considerably over time (Fig 3B and Fig S3). This differed strikingly from recipients receiving the minor fraction of EPCRhighCD48-/low cells, which presented with robust long-term multilineage reconstitution (Fig 3B and Fig S3). While almost no BM cHSC chimerism was observed from EPCRhighCD48+ cells, EPCRhighCD48-/low cells produced very high chimerism in all evaluated progenitor fractions, including for cHSCs (Fig 3C).
These data established that functional in vivo HSC activity following culture is restricted to the minor fraction of SLAM LSK EPCRhigh cells.
Quantification of HSC activity in ex vivo expansion cultures
Expanded cHSCs presented with a vastly higher reconstitution activity compared to freshly isolated cHSCs, and mostly fell out of range for accurate quantification (Fig 3B). We therefore assessed the impact of lowering the amount of input cHSCs and enhancing the amount of competitor cells. Aliquots of 10 cHSCs were cultured and expanded for 21 days. Cells from each culture (EE10) were next mixed with 2 or 20 million (2M or 20M) competitor WBM cells, followed by transplantation (Fig 4A, ‘individual’). In parallel, we also included a group in which EE50 cHSCs were mixed with 10 million WBM cells and split among 5 lethally irradiated recipients (Fig 4A, ‘pooled’). This allowed us to assess the variability among input cHSCs,
Although robust reconstitution was observed in most recipients of ‘individual’ cultures (Table 1, Fig 4B-C, Fig S4A-B), the levels of test-cell derived cells varied extensively among these recipients. To quantify the HSC activity in each recipient, we calculated the repopulating units (RUs)9 for each lineage and recipient separately at the experimental endpoint (Table 1). Short-lived myeloid cells has been proposed as a better indicator of ongoing HSC activity27, and in agreement with this, we observed a high correlation between the RUmyeloid and RUs for cHSCs in the BM (Table 1). A much more consistent HSC activity was observed in between recipients of ‘pooled’ cells (Table 1, Fig 4B-C). This confirmed that the differential chimerism in recipients of limited cHSC numbers relates to an unresolved cellular heterogeneity of input cHSCs. RUs per initial cHSC were calculated for freshly isolated BM cHSCs (the three EPCRhigh groups from Fig 1B) and following 3-week culture (‘pooled’ group). This revealed 461- and 70-fold increases in PB myeloid and BM cHSC chimerism in recipients of ex vivo expanded cells, respectively. This is an estimate of the increase of functional HSC activity following ex vivo culture (Fig S4G).
We next assessed HSC clonality using a lentiviral barcoding approach. Five aliquots of 1,000 cHSCs were transduced with a barcode library28 48 hours after culture and then expanded for 19 days. Half of the expanded cells from each well were transplanted into one ‘parental’ recipient, respectively, while the other half was mixed with each other, with each ‘daughter’ recipient receiving 1/5 of the mixed cells (Fig 4D- i). As a proxy for the HSC activity, barcodes were extracted from BM test-derived myeloid cells 16-weeks post-transplantation. Following stringent filtering, we retrieved 223 unique barcodes in the ‘parental’ recipients, with a highly variable contribution (0.1 - 26.0%, Fig 4E-G). Representation of the same barcode in ‘parental’ and ‘daughter’ recipients demonstrates a shared clonal/HSC origin, where more robustly expanding HSCs should have a higher chance of reconstituting more ‘daughter’ recipients. Our data agreed well with this presumption (Fig 4E), and the most actively shared clones also associated with larger outputs in ‘daughter’ recipients (Fig 4F).
While our initial barcode experiments were designed to assess the in vitro expansion potential of cHSCs, a next set of experiments were executed to assess the clonal HSC activity following expansion. For this, cHSCs were ex vivo expanded for 13 days, provided with barcodes29 overnight, and transplanted into 4 lethally irradiated recipients (Fig 4D-ii). 16-weeks post-transplantation, we recovered 573 unique barcodes. The median clone sizes of HSCs post-culture were significantly smaller than the pre-culture clones (Fig 4G), which was expected as more clones (573 vs 223) were evaluated in the ‘post-culture’ setting. However, the frequency of dominantly contributing clones was significantly less abundant (Fig 4H), demonstrating a more even contribution from individual clones in this setting. This is in line with the interpretation that expanded HSCs are functionally more equivalent than input cHSCs.
Finally, we assessed how the culture system supports the in vivo activity of fetal liver (FL) cHSCs. E14.5 FL-cHSCs were sorted using the same immuno-phenotype as BM cHSCs and cultured ex vivo. We observed an average 188-fold increase of cHSCs following 21-days culture (Fig S4D). We used the ‘pooled’ approach for functional evaluation and competed EE10 cells with either 2 or 20 million WBM cells (Fig S4C). While ex vivo expanded FL-cHSCs contributed considerably to multilineage reconstitution (Fig S4E), we observed compared to adult cHSCs 5.7- and 6.6-fold lower reconstitution from FL-cHSCs within PB myeloid cells and cHSCs, respectively (Fig S4F-G).
Taken together, these experiments demonstrated robust increases in HSC activity following culture of cHSCs, albeit slightly less for FL-cHSCs. However, clonal barcode assessments revealed substantial variation in ex vivo expansion potential of even stringently purified input cHSCs.
Ex vivo expanded cHSCs returns to a quiescent state following engraftment in unconditioned recipients
Successful engraftment in an unconditioned setting requires large numbers of HSCs/HSPCs16, which reportedly can be accommodated by PVA-cultured HSCs3. To test this, we transplanted EE100 CD45.1+ cHSCs into lethally irradiated or unconditioned CD45.2+ recipients (Fig S5A). In lethally irradiated hosts, cultured cells reconstituted >90% of PB cells, including with robust contribution of donor cells to the myeloid lineages. By contrast, most of the unconditioned recipients lacked long-term reconstitution (Fig S5B). Given that CD45 mismatching might be a sufficient immunological barrier to prevent HSPC reconstitution30, we instead transplanted expanded cells into unconditioned F1 CD45.1+/CD45.2+ hosts (Fig 5A). Clear long-term multilineage engraftment was observed in all these hosts (Fig 5B).
In the BM, cHSCs exist for the most part in a quiescent state, which contrasts the situation in cultures (Fig S5C). To investigate to what extent ex vivo expanded cHSCs could return to quiescence following transplantation, we expanded 100 CD45.2+ cHSCs for 21 days and stained all their progeny with the proliferation-tracking dye Cell Trace Violet (CTV) prior to transplantation into CD45.1+/CD45.2+ hosts. As a control, we transplanted CTV labelled unmanipulated CD45.2+ cKit-enriched BM cells (Fig 5C). In these animals, splenic CD4+ enriched cells were co-transplanted as a control for non-dividing cells (Fig S5D). Reconstitution and cHSC fates were monitored biweekly for up to 8-weeks post-transplant. Although the phenotypic cHSC contribution in recipients receiving ex vivo expanded cells outnumbered the contribution from unmanipulated cells two-weeks post-transplantation, the levels became comparable later (Fig 5D). Recipients from both groups showed high levels of CTV signal two- weeks post-transplantation, with the majority of donor EPCR+ cHSCs having undergone fewer than 3 cell divisions. Over time, increasing numbers of cHSCs had proliferated within both groups and with similar patterns (Fig 5E-F, Fig S5E), with a slightly higher number of undivided cHSCs retrieved from ex vivo expanded HSCs at all time points (Fig 5E). The most quiescent cHSCs associated with higher EPCR levels (Fig S5F-G), again attesting to the usefulness of EPCR to predict robust HSC activity.
Therefore, ex vivo expanded cHSCs can efficiently engraft immunologically matched hosts without conditioning. Under such conditions, expanded cHSCs can rapidly return to quiescence following transplantation.
Although many efforts have aimed to in vitro expand murine HSCs, the results have for the most part been disappointing31. A recent study suggested that the substitution of undefined serum products with the synthetic polymer PVA is one key determinant towards this goal3. Here, we detailed this culture system further. We focused on the requirements of candidate input HSCs, the reproducibility of the system, the cellular output and the functional in vivo performance of the in vitro replicating HSCs. Several observations that we believe are valuable not only for future applications of the PVA culture system, but also for HSC biology in general, emerged from our work.
While the PVA-system supports HSC activity even from unfractionated BM cell preparations32, the long-term repopulating HSCs (LT-HSCs) in cruder input cell preparations is very low. While having its own advantages, this makes it difficult to track the fates of cHSCs. Therefore, we here consistently initiated cultures with more stringently purified cHSCs. As a starting point, we used cHSCs isolated based on a current standard SLAM phenotype (Lin-Sca+cKit+CD48-CD150+)33. In agreement with previous reports34, we observed that CD201/EPCR further enriches for LT-HSCs, a knowledge that has so far not been adopted as a standard in the field33. This applied both to cHSCs isolated directly from the BM and following culture. In fact, despite that HSCs change many phenotypes in culture4, the LT-HSC activity following culture was remarkably enriched not only in the EPCR positive fraction, but also for other phenotypic attributes of unmanipulated LT-HSCs. Importantly, such cHSCs represented only a very minor fraction in cultures (0.6% and 0.1% of 2- and 3-week cultures, respectively) but contained all LT-HSC activity, with the massive overall expansion in cultures leading to substantial net expansions of such cells (100- to 1,200-fold).
While we gained insights into the dynamics of ex vivo differentiation of cHSCs into mature lineages by simultaneously measuring ATAC signal and RNA transcripts in individual nuclei using single-cell multiome sequencing, the limited sensitivity of this method hindered the identification of more homogeneous populations of bona fide HSCs within the sorted EPCR+ cells. Therefore, we assessed the functional in vivo HSC activity in most of our subsequent work. While our clonal barcoding experiments unequivocally demonstrated HSC self-renewal, the large spectra in expansion potential of individual cHSCs was noteworthy. This variation was reduced when assessing HSC activity following culture and aligns with the interpretation that the offspring of cHSCs that self-renew robustly in cultures exhibit less differences. Nonetheless, the demonstrated in vitro self-renewal refutes alternative views in which the culture process would mainly act to enhance the reconstitution capacity of individual HSCs, for instance by altering their homing properties and/or by the conversion of more differentiated progenitors into cells with LT-HSC activity. Therefore, our data support the view that this (or other) culture systems might not support all types of LT-HSCs previously alluded to35. Further support to this notion can be derived from our data on FL-cHSCs, which did not expand better than adult HSCs in the PVA system, despite that FL HSCs repopulate mice more efficiently than adult HSCs when unmanipulated36. In a broader sense, this associates also with the general concept of self-renewal and that has recently been highlighted in studies on native hematopoiesis, where distinct but only slowly contributing LT-HSCs co-exist with more active progenitors that can presumably also execute self-renewal divisions37–39. It appears that many aspects of this structure are re-created in cultures and, as we show, this requires as input the normally very slowly dividing and presumably most primitive LT- HSCs.
While the parallel self-renewal and differentiation in HSC cultures represent a fundamental difference compared to other in vitro stem cell self-renewal systems (e.g., for ES/iPS cells), where preferential self-renewal can be achieved, the differentiation of HSCs can be taken advantage of. We observed that even single cultured HSCs can enhance the speed of donor reconstitution and alleviate the effects of myeloablative conditioning, and the parallel self-renewal and differentiation can also be harnessed to approach HSC fate decisions at a molecular level40. This was exemplified by our single-cell multimodal experiments, which allowed for determinations of the differentiation trajectories of cHSCs in the culture system and their associated molecular features. However, as the expansion of genuine LT-HSC activity is far from unlimited, a shortage of HSCs is by all likelihood still an obstacle for larger-scale screening efforts such as genome-wide CRISPR screens and larger small molecule screens.
During our studies, we encountered several aspects of the CRA that are often neglected. First, the HSC activity when transplanted at limited numbers highlighted large variations in between recipients, which is in line with previous extensive single cell transplantation experiments41–45. While raised previously46, taking into account not only the repopulating activity but also clonal aspects has not reached wide use. Emerging barcoding technologies, by which many clones can be evaluated in a single mouse, makes this easier. Secondly, the progeny from a relatively small number of cultured cHSCs (10-50 cells) vastly outcompeted “standard” doses of competitor cells, making quantification unreliable/impossible. This contrasted results from freshly isolated cHSCs transplanted at the same doses used to initiate cultures. While this concern could be overcome by adjusting the graft composition (e.g. enhancing the number of competitor cells), extensive variation still existed among cultures that could be eliminated by using larger amounts of input cells and splitting the contents into individual recipients. This should have relevance also for normal HSC biology by highlighting intrinsic rather than stochastic regulation as the central determinant for persistent HSC function. A third consideration relates to the definition of ongoing HSC activity. We and others have previously established that this is best mirrored by the HSC contribution to myelopoiesis47,48. When we assessed cultured EPCR+CD48+ cells (with predominantly transient multilineage activity), we observed prominent long-term T cell contribution but limited myeloid reconstitution. This should not be confused with the concept of HSC lineage-bias, but rather reflects that the pool of T cells in vivo can be maintained by homeostatic proliferation and thus do not require continuous input from HSCs. Thus, short-lived non-self-renewing multipotent progenitors, which are generated in large numbers in culture, can contribute extensive (lymphoid) offspring in the long-term but should not be mistaken for ongoing HSC activity. This consideration is valid also for competitor grafts in the CRA (in the form of WBM cells), which contain numerous progenitor cells that can rapidly and persistently contribute to lymphopoiesis. Thus, as opposed to the classic CRA, where the overall donor contribution is assessed9, the contribution to short-lived lineages reflects better the ongoing HSC activity, with the contribution to lymphoid lineages serving as a qualitative parameter for multipotency. In agreement with previous viral barcoding studies on HSCs14 we observed that when cultured HSCs are barcoded and assessed long-term, there is a strong correlation between barcodes in BM progenitors and myeloid cells, while the pool of mature B cells contain clones that cannot be recovered in other lineages (data not shown).
Successful repopulation of non-conditioned hosts is based on the suggestion that large numbers of HSCs are needed to saturate those few BM niches available for engraftment18,49. Avoiding toxic myeloablation has apart from evident clinical implications also a precedence in experimental murine HSC biology, where lethal myeloablation enforces vigorous HSC proliferation following transplantation12, but which contrasts native contexts12,37,38. We observed that the CD45.1/CD45.2 differences are sufficiently immunogenic to mediate long-term graft failure in the non-conditioned setting, which in hindsight has been alluded to previously18,30,50. Thus, by matching hosts for CD45-isoforms, we could successfully obtain low-level (2-5%) long-term multilineage chimerism in adult non-myeloablated hosts from only 100 cHSCs. Intriguingly, despite being activated to proliferate in vitro, many cHSCs rapidly returned to a quiescent state in vivo, with the most dormant cHSCs exhibiting the most stringent HSC phenotype (EPCRhigh SLAM LSKs). The possibility for non-conditioned transplantation should complement genetic lineage tracing models aimed at exploring both normal and malignant hematopoiesis in more native/physiological contexts12,37,38. In summary, we here characterized and detailed murine HSCs as they self-renew and differentiate in vitro. Although several aspects of the PVA system remain to be explored, a powerful in vitro self-renewal system has been a long-sought goal in HSC biology where HSCs have been notoriously difficult to uphold in an undifferentiated/self-renewal state. The merger of this system with recent advances in transplantation biology, genome engineering and single-cell techniques holds promise for many exciting discoveries of relevance to both basic and more clinically oriented hematopoietic research.
Materials and methods
Adult (8-10-week-old) C57Bl/6N (CD45.2+) female mice were purchased from Taconic. Transgenic Fgd5-ZsGreen-2A-CreERT2 mice26 (JAX:027789) was a kind gift from Derrick Rossi. Mice were maintained in the animal facilities at the Biomedical Center of Lund University and kept in environment-enriched conditions with 12-hour light-dark cycles and water and food provided ad libitum. All experimental procedures were approved by a local ethical committee (permits M186-15 and 16468/2020). All efforts were made to reduce the number of experimental animals and suffering.
All mice used as recipients were 8 - 12 weeks of age. For conditioned recipients, mice were lethally irradiated (950 cGy) at least 4 h prior transplantation. The conditioned mice received prophylactic ciprofloxacin (HEXAL, 125 mg/L in drinking water) for 2 weeks beginning on the day of irradiation. All transplantations were performed through intravenous tail vein injection. The amount of transplanted test cells and competitor/support cells is described in the results section.
In vitro HSC culture
In vitro HSC cultures were performed using F12-PVA based culture conditions as previously described3. In brief, cHSCs (HSCs (EPCRhighCD150+CD48-/low LSKs) were sorted into 96-well flat-bottom plates (Thermo Scientific) coated with 100 ng/ml fibronectin (Sigma) for >1 hour at 37 °C prior to sorting. HSCs were sorted into 200 µl HSC media (Supplemental Table 1) and expanded at 37°C with 5% CO2 for up to 21 days. The first media changes were performed 5 days (for ≥ 50 initial HSCs), 10 days (for 10 initial HSCs) or 15 days (for single-cell cultures) after initiation of cultures and then every 2 days. For pre-cultures associated with lentiviral transduction, the first media change was performed 24h after transduction and then as above. Cells were split at a 1:2-1:4 ratio into new fibronectin coated plates when reaching 80-90% confluency (normally every 4 days after the first split). In order to expand cHSCs to reach a workable number as well as avoiding vast numbers of differentiated cells generated after very long-term of culture (f.i. up to 4 weeks), the cHSCs were kept expanding ex vivo for 3 weeks as a standard protocol. Following 14- and/or 21-day expansion, cellularity was assessed using an Automated Cell Counter (TC20, BioRad) and used for flow cytometric analyses and/or transplantation.
Mice were euthanized by cervical dislocation after which bones (both right and left tibias, femurs, and pelvis) or spleens were extracted. Fetal cells were extracted from livers at E14.5. Bones were crushed using a mortar and pestle and BM cells were collected in ice-cold phosphate-buffered saline (PBS, Gibco) with 1% fetal bovine serum (Sigma-Aldrich) (FACS buffer), filtered (100 µm mesh) and washed. FL or spleen cells were brought into single cell suspension by grinding through a 100 µm mesh. BM or FL cells were cKit-enriched by anti-cKit-APCeFluor780 (eBioscience) staining and spleen cells were CD4-enrihced by anti-CD4-APC-Cy7 staining, followed by incubation with anti-APC conjugated beads (Miltenyi Biotec). Magnetic separation was performed using LS columns according to manufacturer’s instructions (Miltenyi Biotec). cKit-enriched BM or FL cells were washed and stained with fluorescently labeled antibodies for analysis or sorting.
Peripheral blood (PB) chimerism analysis after transplantation was done as previously described39. In brief, blood was drawn from tail vein into FACS buffer containing 10 U/ml Heparin (Leo). After incubating with an equal volume of 2% dextran (Sigma) in PBS at 37 °C, the upper phase was collected and erythrocytes were lyzed using an ammonium chloride solution (STEMCELL Technologies) for 3 min at room temperature, followed by washing.
For analysis and/or sorting after ex vivo expansion, cultured cells were resuspended by pipetting and collected using FACS buffer. An aliquot was taken for cell counting using an Automated Cell Counter (TC20, BioRad).
Flow cytometry analysis and FACS sorting
Cells were kept on ice when possible, with the FACS buffer kept ice-cold. Staining, analysis and sorting were performed as previously described12. In brief, cells were resuspended in Fc-block (1:50, 5 x 106 cells/50 µl, InVivoMab) for 10 min and then for 20 min with a twice concentrated antibody staining mixture (Supplemental Tables 2-10) at 4 °C in dark. In case biotinylated lineage markers were included, a secondary staining with streptavidin BV605 was performed for 20 min (1:400, 5 x 106 cells/100 µl, Sony) at 4 °C in dark. After a final wash, the cells were resuspended in FACS buffer containing propidium iodide (1:1000, Invitrogen). All FACS experiments were performed at the Lund Stem Cell Center FACS Core Facility (Lund University) on Aria III and Fortessa X20 instruments (BD). Bulk populations were sorted using a 70-micron nozzle, 0.32.0 precision mask, and a flow rate of 2 - 3K events/s. HSCs for single cell culture were index sorted. FACS analysis was performed using FlowJo version 10.8.0 (Tree Star Inc.).
Multiome single-cell sequencing
Multiome sequencing experiments were performed at the Center for Translational Genomics (Lund University) using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle kit according to manufacturer’s instructions (10x Genomics). 40,000 viable cells or EPCR+ viable cells were sorted from ex vivo expanded cultures for multiome single-cell sequencing. Data has been uploaded to GEO under accession number GSE234906 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE234906) and will be available upon publication.
All accompanying code for the single-cell multiome analysis post Cell Ranger can be found at this Github repository (https://github.com/razofz/DB_QZ_multiome), including conda specifications for the environments used with exact versions of all packages.
The count matrices were generated with the Cell Ranger ARC tool (v2.0.0), aligned to the reference genome mm10. The aggregated dataset (containing data for both samples) were then loaded into Signac/Seurat in R where non-standard chromosomes were removed and the Seurat object split into the two samples, here named Diverse and Immature, which corresponds to whole culture or sorted EPCR+ cells, respectively. The data were then filtered on UMI, gene and peak counts, as well as nucleosome signal and TSS enrichment, with separate thresholds for the two samples. The ATAC features were classified as either distal or proximal peaks, and split accordingly. Proximal peaks were defined as a ± 2 kbp region around the transcription start site (TSS). The TSS positions were retrieved from GREAT’s website, and GREAT v4 mm10 version was used. All peaks not classified as proximal received the classification “distal”. Motifs were then identified for distal and proximal peaks separately51.
ii. Downstream processing and joint-modality dimensionality reduction
The samples were processed separately. Since SAILERX uses pre-existing dimensionality reduction and clusters for the RNA modality, the RNA data was normalized and scaled, highly variable genes (HVGs) were found, principal component analysis (PCA) was run and clustering was performed, all with the Seurat standard functions with default parameters. After that, relevant data was extracted (metadata including RNA clusters, PCA embeddings, a whitelist of non-filtered out cell barcodes, ATAC peak count matrix) and with it an hdf5 file similar to the example files provided by the SAILERX repository was created (https://github.com/uci-cbcl/SAILERX). A SAILERX model was then trained on the generated hdf5 files (still sample-wise) according to their provided instructions. The resulting embeddings from the SAILERX joint-modality dimensionality were then exported and imported into the respective Seurat objects. In Seurat, the embeddings were used for graph construction, clustering and constructing UMAPs. Scores for cell cycle gene signatures were calculated and visualized (Fig 2E for the Immature sample) using Seurat’s corresponding standard functions with default parameters.
iii. Differential gene expression
Differential gene expression testing was performed on the resulting clusters using Seurat’s FindAllMarkers function with the HVGs found above. This analysis was used to calculate HSC signature score as described below (v. HSC signature).
iv. ATAC processing and cluster annotation
Motif activity scores were identified with chromVAR52 for distal and proximal peaks respectively, still sample-wise. Differentially active motifs (DAMs) were then identified for each cluster. Annotation of clusters in terms of cell type was then performed manually with the help of DEGs and DAMs (Fig 2B), guided by preexisting data on the expression profiles of cell types using the Enrichr platform (https://maayanlab.cloud/Enrichr/).
v. HSC signature
A 13 gene condensed HSC signature list (Fig S2C) was obtained by mining genes associated to cluster 5 with publicly available gene expression data of HSCs from the ImmGen consortia and Bloodspot (https://servers.binf.ku.dk/bloodspot/). This signature was then used to calculate a score with Seurat’s AddModuleScore function. A classification for expression of this gene signature was performed with a slightly modified version of Seurat’s CellCycleScoring function, where instead of three classifications (S, G2M or Undecided/G1) two classifications were used (expressed or not expressed). This classification was then used to create the contours used in Fig 2D-E, and the signature score used for coloring the points in the same plots.
vi. Trajectory inference in the Diverse sample
Trajectory inference was performed for the Diverse sample with the slingshot package53. As initiating cluster, the cluster 5 was assigned (the earliest cluster), and end clusters chosen were clusters 4, 1, 6 and 7. The slingshot function of slingshot was used with otherwise default parameters. A joint UMAP with the four resulting trajectories was constructed (Fig 2C). To circumvent the problem of interfering color scales, only the top 20% expressing cells for each marker was chosen for coloring, and overlapping cells between these sets were excluded.
Assessment of HSC heterogeneity via DNA barcoding
Lentiviral barcode libraries were purchased from AddGene (No. 115645 and No. 83993) and amplified according to instructions28,29. AddGene 83993 is a Crispri gRNA library that was repurposed here for the purpose of lentiviral barcoding (e.g. no Cas9 was co-expressed). These libraries were kind gifts from Samantha Morris and Jonathan Weissman.
For pre-culture labelling, five wells containing 1000 CD45.1+ HSCs each were sorted and cultured for 48 h. Cells were then transduced with the AddGene 115645 library (containing >5737 unique barcodes) overnight with approximately 35% transduction efficiency, which was followed by regular culture procedures. After 19 days, expanded cells were collected from each initial wells separately. Half of the expanded cells were transplanted into individual ‘parental’ CD45.2+ recipients and the rest were mixed together and transplanted into five ‘daughter’ CD45.2+ recipients. Each ‘parental’ or ‘daughter’ recipient received an estimated expansion equivalent to 500 cHSCs. For post-culture labelling, 3000 cHSCs were sorted from CD45.1+ mice and expanded for 13 days. Thereafter, the cells were transduced with the AddGene 83993 library (containing >10090 unique barcodes) overnight at an approximate density of 100,000 cells/well. Half of the cultured and transduced cells were transplanted into 5 CD45.2+ WT recipients. Each recipient received an estimated expansion equivalent of 300 cHSCs, of which approximately 15% were transduced according to assessments of transduction efficiencies. Barcode labelled cells were collected and analyzed from four recipients. For both pre- and post-culture labeling experiments, recipients were lethally irradiated and provided with 500,000 unfractionated CD45.2+ WBM cells each as support. At the experimental end points (16 weeks after transplantation), barcode labelled BM myeloid cells were sorted into 200 µl RNA lysis buffer (Norgen Biotek Corp) (“pre-culture” barcoding) or pelleted for genomic DNA extraction (“post-culture” barcoding) and proceeded to library preparation and sequencing analysis.
RNA was isolated according to the protocol of Single Cell RNA Purification Kit (Norgen Biotek Corp) and cDNA were synthesis using qScript cDNA SuperMix (Quantabio). Genomic DNA was extracted using PureLink Genomic DNA Mini Kit (Invitrogen). The 8- (pre-culture) and 21-base pair (bp) barcodes (post-culture) were amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs). Primers used are listed in Supplemental Table 11. Adapters containing index sequences (Supplemental Table 10) were added by PCR for 7 cycles. After each PCR amplification step, the products were cleaned up using SPRIselect beads (Beckman Coulter). Purified libraries were run on Agilent High Sensitivity DNA Kit chip (Agilent Technologies) to verify the expected size distribution, quantified by Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific), and pooled at equimolar concentrations. Pooled libraries were loaded on an Illumina MiSeq Reagent Nano Kit v2 flow cell following protocols for single-end 100-cycle sequencing. FASTQ files were further assessed with FastQC (v.0.11.2), including read count, base quality across reads, and guanine and cytosine (GC) content per sequence.
After extracting the reads of each barcode, the frequency in each recipient was calculated and barcodes with a frequency less than 0.1% were considered background reads. This is an arbitrary cut-off. Barcodes present in more than one ‘parental’ recipient were excluded from analysis (7 % and 2% of barcodes pre- and post-culture, respectively) to avoid the possibility of including HSCs transduced independently with the same barcode or potential over-represented barcodes. Reads were normalized to 106 total reads per recipient followed by statistical analyses.
Cell Trace Violet labelling for in vitro and in vivo HSC proliferation tracing
CD45.2+ cultured cells, cKit-enriched BM cells or CD4-enriched spleen cells were collected, washed and pelleted. Cells were resuspended to a final concentration of 5 x 106 cells/ml and labelled with 1 µM CTV (Invitrogen) in PBS for 10 min at 37 °C. The reaction was stopped by adding same volume of ice-cold FACS buffer and washing again with FACS buffer. For in vitro proliferation tracing, 105 CTV labeled cKit-enriched BM cells were plated into each well containing F12-PVA culture media (Supplemental Table 1). At each time point, half of the cultured cells were collected and stained for evaluating cHSC CTV signals (Supplemental Table 8) while the remaining cells were kept in culture for continue expansion. For in vivo proliferation tracing, CTV labeled EE100 cultured cells or 5 x 106 cKit-enriched BM cells were mixed with 2 x 106 CTV labeled CD4-enriched spleen cells and transplanted into one CD45.1+/CD45.2+ unconditioned recipients. For analysis, BM cells were cKit-enriched and stained accordingly (Supplemental Table 9). CD4 positive spleen cells were collected from the same recipient as a control for positive signal/undivided cells (Supplemental Table 10).
Statistical analyses were performed using Microsoft Excel and Graphpad Prism 9. Significance was calculated by Mann-Whitney tests. Statistical significances are described in figure legends for the relevant graphs. In all legends, n denotes biological replicates.
We acknowledge Dr. Shabnam Kharazi for scientific discussions and technical support, Mr. Yun Sheng for advice on analysis for barcode sequencing and Prof. Hiromitsu Nakauchi and Dr. Adam C. Wilkinson for advice on cell cultures. The work was supported by grants from the Tobias Foundation, the Swedish Cancer Foundation, Barncancerfonden and the Swedish Research Council to D.B. and the Royal Physiographic Society of Lund foundation to Q.Z and A.K-C.
Q.Z. designed the research, performed experiments, analyzed data, and wrote the manuscript; R.O. performed bioinformatic analysis and wrote the manuscript; A.K-C. and O.Y. performed experiments and analyzed data; D.B. supervised the project, was responsible for funding acquisition, designed the research, analyzed data and wrote the manuscript.
Conflict of interest disclosures
The authors report no conflict of interest.
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