Chromatin accessibility dynamic during GABAeric interneuron differentiation
A. Schematic illustration of GABAergic interneuron differentiation protocol. Cells from iPSC differentiation were collected for ATAC-Seq. Interneuron differentiation was grouped into four stages: iPSC (Day 0), neural progenitor cells; NPCs (Day 19), immature neurons, imN (Day 35), GABAergic interneurons, GABA (Day 65).
B. Heatmap of four Clusters identified by time series analysis using temporal changes in chromatin accessibility compared to D0 (Day 0) and other points. The signal strengths were denoted by color intensities.
C. Line plot of four clusters showing the average changes in chromatin accessibility.
D. Barplot for genomic distribution of differential chromatin accessible regions for each cluster.
E. Heatmap for top 10 TFs enrichment in each cluster. The significance was denoted by color intensities. O-S-E-N = OCT4-SOX2-TCF-NANOG.
F. Bubble plot of KEGG pathway enrichment for each cluster. p-value and enrichment were indicated.
G. Genome browser view showing representative differential chromatin accessible regions at the indicated gene loci.