Knockdown of FMNL2 disrupts actin assembly during mouse oocyte meiosis.
(A, B) Representative images of actin distribution at the oocyte cortex and the fluorescent intensities in the control group and FMNL2-KD group (P > 0.1). White, actin; green, tubulin; magenta, DNA. Bar = 10 μm. (C, D) Representative images of actin distribution in the oocyte cytoplasm and the fluorescent intensities in the control group and FMNL2-KD group. White, actin; green, tubulin; magenta, DNA. Bar = 10 μm. (E, F) Representative images of actin distribution in the oocyte cytoplasm and the fluorescent intensities in the FMNL2-KD group and rescue group. White, actin; magenta, DNA. Bar = 10 μm. (G) Mass spectrometry results showed that FMNL2 was related to many actin-related proteins. (H) Co-IP results showed that FMNL2 was correlated with Arp and Formin2 but not with Profiling and Fascin. (I) Arp2 protein expression significantly increased in the FMNL2-KD oocytes compared with the control oocytes. Arp2 protein expression significantly decreased in the rescue oocytes compared with the FMNL2-KD oocytes. (J) Formin2 protein expression significantly decreased in the FMNL2-KD oocytes compared with the control oocytes. Formin2 protein expression significantly increased in the rescue oocytes compared with the FMNL2-KD oocytes. The data are presented as mean ± SEM from at least three independent experiments. * P < 0.05, ** P < 0.01, **** P < 0.0001.