The binding sites of modified LDLs on SCARF1
(A) Surface electrostatic potential of f2 fragment shows a positively charged region on SCARF1 (left, red rectangle), which contains four arginines at site 1 and site 2 (right, magenta ovals).
(B) Interactions of AcLDL with the cells transfected with the single mutants (R to S) of the binding sites by flow cytometry.
(C) Interactions of OxLDL with the cells transfected with the single mutants (R to S) of the binding sites by flow cytometry.
(D) Interactions of AcLDL with the cells transfected with the double, triple or quadruple mutants (R to S) of the binding sites by flow cytometry.
(E) Interactions of OxLDL with the cells transfected with the double, triple or quadruple mutants (R to S) of the binding sites by flow cytometry.
(F) Interactions of AcLDL with the cells transfected with the double or quadruple mutants (R to K) of the binding sites by flow cytometry.
(G) Interactions of OxLDL with the cells transfected with the double or quadruple mutants (R to K) of the binding sites by flow cytometry.
(H) Confocal fluorescent images of the SCARF1 mutant transfected cells incubated with OxLDL (scale bar, 7.5 µm).