Structural Biology and Molecular Biophysics

Structural Biology and Molecular Biophysics

eLife publishes research that uses techniques including X-ray crystallography, cryo-EM and single-molecule methods. Learn more about what we publish and sign up for the latest research.
Illustration by Davide Bonazzi

Latest articles

    1. Structural Biology and Molecular Biophysics

    A cryogenic, coincident fluorescence, electron, and ion beam microscope

    Daan B Boltje, Jacob P Hoogenboom ... Sander den Hoedt
    Integrating a cryogenic light microscope with a focused ion beam/scanning electron microscope, allows directly targeting of fluorescent structure when preparing a frozen-hydrated lamella, without the need for repositioning or fiducial markers.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Defocus Corrected Large Area Cryo-EM (DeCo-LACE) for label-free detection of molecules across entire cell sections

    Johannes Elferich, Giulia Schiroli ... Nikolaus Grigorieff
    Cryo-electron microscopy can be used to detect biomolecules in entire 100- to 250-nm-thick cell slices when using a method to collect montages that prevents radiation damage in areas that have not been imaged yet.
    1. Structural Biology and Molecular Biophysics

    Structure of the GOLD-domain seven-transmembrane helix protein family member TMEM87A

    Christopher M Hoel, Lin Zhang, Stephen G Brohawn
    A cryo-EM structure of TMEM87A unexpectedly reveals it is structurally related to seven other proteins including Wntless, a chaperone for lipidated and secreted Wnt morphogens, suggesting GOLD-domain seven-transmembrane helix (GOST) proteins may all similarly traffic membrane-associated cargo.
    1. Immunology and Inflammation
    2. Structural Biology and Molecular Biophysics

    Structure of human phagocyte NADPH oxidase in the resting state

    Rui Liu, Kangcheng Song ... Lei Chen
    The cryo-EM structure of a functional human NOX2-p22 complex in nanodisc in the resting state reveals the architecture of this key enzyme essential for innate immunity.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    The Nse5/6-like SIMC1-SLF2 complex localizes SMC5/6 to viral replication centers

    Martina Oravcová, Minghua Nie ... Michael N Boddy
    SIMC1 and SLF1 bind exclusively to SLF2 to form two separate complexes that direct the human SMC5/6 complex to its antiviral defense or DNA lesion repair activities.
    1. Structural Biology and Molecular Biophysics

    Sublytic gasdermin-D pores captured in atomistic molecular simulations

    Stefan L Schaefer, Gerhard Hummer
    Molecular dynamics simulations reveal the formation of membrane pores by gasdermin-D, the ultimate effector of pyroptotic cell death in inflammation and infection.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Reevaluation of Piezo1 as a gut RNA sensor

    Alec R Nickolls, Gabrielle S O'Brien ... Alexander T Chesler
    Piezo1 ion channels are not modulated by RNA ligands, contrary to previous findings.
    1. Structural Biology and Molecular Biophysics

    Dilated cardiomyopathy mutation E525K in human beta-cardiac myosin stabilizes the interacting-heads motif and super-relaxed state of myosin

    David V Rasicci, Prince Tiwari ... Christopher M Yengo
    A dilated cardiomyopathy mutation can stabilize the auto-inhibited super-relaxed state and interacting-heads motif in human cardiac myosin, suggesting a mechanism by which disease mutations can reduce muscle force and power.
    1. Structural Biology and Molecular Biophysics

    Structural and thermodynamic analyses of the β-to-α transformation in RfaH reveal principles of fold-switching proteins

    Philipp K Zuber, Tina Daviter ... Stefan H Knauer
    The β-barrel conformation of the KOW domain of the metamorphic protein RfaH is in equilibrium with a low-populated, predominately unstructured state exhibiting helical elements, suggesting that transiently structured elements in unfolded conformations might be a general scheme in fold-switching proteins.
    1. Structural Biology and Molecular Biophysics

    Structural and functional insights of the human peroxisomal ABC transporter ALDP

    Yutian Jia, Yanming Zhang ... Guanghui Yang
    The Cryo-EM structural and biochemical analysis provides a framework for understanding the working mechanism of ALDP.
    Short Report Updated
    Formats available:
    • HTML
    • PDF

Senior editors

  1. Richard Aldrich
    Richard Aldrich
    The University of Texas at Austin, United States
  2. Amy H Andreotti
    Iowa State University, United States
  3. Nancy Carrasco
    Vanderbilt University School of Medicine, United States
  4. See more editors