Cryo-EM and functional studies reveal how combined action of proteins RPS26/eS26 and RIO1 allows late precursors to the human small ribosomal particle to be matured into fully translation-competent 40S subunits.
Structure modeling, site-directed mutagenesis, and current recordings revealed the mechanism by which stabilization of voltage sensors in the resting and activated states determines the gating properties of the CaV1.1 calcium channel.
The structure of gp41with its membrane anchors highlights the flexible linkage of the transmembrane regions and the fuson peptides, which generates an asymmetric conformation, a potential target of MPER bNAbs.
A real-time, stopped-flow method, in combination with protein-induced fluorescence enhancement, Förster resonance energy transfer, and time-resolved fluorescence spectroscopy, reveals a kinetic framework for understanding Dicer as a complex molecular motor.
A combination of equilibrium and nonequilibrium molecular dynamics simulations is an effective tool to study allosteric communications in ultrafast enzymes that show little or no conformational changes.