The procedure for ChIP-seq of flag-tagged proteins and Overview of ChIP-seq data
Validation of procedure for ChIP-seq of flag-tagged cyAbrB2, SigE, and SigA. (A) The immunoblot for inputs and immunoprecipitants (IP) of ChIP for cyAbrB2-FLAG and cyAbrB1-FLAG. Input lysate of untagged control (GT) is also loaded. Inputs equivalent to the indicated portion of IP were loaded. (B)Scatter plots showing the reproducibility of two replicates for ChIP-seq assay. ChIP-seq data of SigE, SigA, and cyAbrB2 in aerobic and microoxic conditions and ChIP-seq data of cyAbrB1 in the aerobic condition are shown. Dots indicate normalized IP read count / normalized input read count in each 100bp window. X-axis is the value of replicate1, and Y-axis is the value of replicate 2. (C) and (D) Overview for ChIp-seq of flag tagged cyAbrB2, cyAbrB1, SigE, and SigA. Y-axis indicates [ normalized IP read count / normalized input read count at each 25bp window], and X-axis indicates chromosome position. (C) Distribution of cyAbrB2, cyAbrB1, SigE, and SigA across the whole genome of Synechocystis. Aerobic (L +O2) and dark microoxic (D -O2) data are displayed. (D) Magnified image for chromosome position of 1,550kb-1,800kb. ChIP-seq data of cyAbrB2, cyAbrB1, SigE, and SigA in aerobic and dark microoxic conditions are overlayed. The dots below the graph indicate the binding region of each protein calculated by peak caller.