Acetylation of the circadian transcription factor BMAL1 by the acetyltransferase TIP60 is crucial for recruitment of the pause release factors to clock gene promoters and productive elongation of these genes.
Multiple enhancers in physical proximity can reinforce shared transcriptional 'hubs' to preserve their transcriptional output, providing a buffer during environmental stresses and genetic perturbations to preserve phenotypic robustness.
Live-cell imaging shows that the contractile acto-myosin network on the nuclear envelope remnant positions chromosomes in early mitosis to ensure efficient and correct interactions between chromosomes and the mitotic spindle.
For the first time, the spectrum of genes and pathways interacting with alternative DNA structures called G-quadruplexes in humans is revealed highlighting new possibilities for the pursuit of cancer therapies.
Enhancements to the CUT&RUN antibody-targeted nuclease strategy for chromatin profiling include an improved affinity-cleavage fusion protein and digestion protocol, and a simplified calibration strategy.