HMMR regulates the dynamics of neuronal microtubules.
(A) Representative image of a 4DIV EB3-mCherry-expressing cortical neuron. The color boxes indicate regions of quantification: red, green, and blue boxes represent the distal, middle, and proximal neurite, respectively. The scale bar presents 10 µm. (B) Representative kymographs of indicated neurons at different regions of the neurite. (C) Quantification of EB3-mCherry comets dynamics in B. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001, one-way ANOVA followed by Dunnett’s post-hoc tests. (D) Representative kymographs of indicated neurons at different regions of the neurite. (E) Quantification of EB3-mCherry comets dynamics in D. ****, P<0.0001, two-tailed Student’s t-test. At least 15 neurons were analyzed per condition per repeat. All bar graphs are expressed as mean ± s.e.m. from three independent repeats.