Characterization of the immune response of dermal fibroblasts in ACD
(A) tSNE plots showing cell distribution of the Pdgfra+ dermal fibroblasts after re-clustering.
(B) Bubble plots showing the expression of marker genes for each dFB cell cluster. Abbreviations: AP, adipocyte progenitors; Areg, adipogenesis-regulatory cells; pAd, preadipocytes; RET/PAP, reticular and/or papillary dFBs; pF, peri-follicular dFBs.
(C) Violin plots showing the expression of indicated genes across various dFB sub-populations in the control and the ACD samples.
(D) SCENIC analysis showing the top enriched transcriptional factors in various dFB clusters.
(E) GO pathway analysis of the upregulated genes (red) or downregulated genes (blue) in the r5 dFB cluster after ACD elicitation.
(F) Volcano plot showing differentially expressed genes in control and ACD samples within the r5 dFB cluster.
(G) Violin plots showing the expression of indicated genes across various cell populations in the control and the ACD samples.
(H) Frozen sections of control and ACD ear skin samples were subjected to immunostaining analysis using antibodies against CXCL9 (green) and PDGFRA (red). Nuclei were counter stained by DAPI (blue). Dermal CXCL9+ or PDGFRA- cells were highlighted by either red- or green-dotted lines in the zoom-in panel. Scale bar, 200 μm. Zoom-in image is shown on the right-hand side.
(I-J) Quantified results showing the fluorescent intensity (arbitrary unit, AU) of CXCL9, CXCL10 or PDGFRA in the dermal CXCL9/10+ cells or PDGFRA- cells shown in Fig. 4H or Fig. S4G.
All error bars indicate mean ± SEM. *p < 0.05, ***p < 0.001, ****p < 0.0001, ns, non-significant.
Figure supplement 1. Characterization of the immune response of dermal fibroblasts in ACD.