Abstract
Saliva is essential for oral health. The molecular mechanisms leading to physiological fluid secretion are largely established, but factors that underlie secretory hypofunction, specifically related to the autoimmune disease Sjögren’s syndrome (SS) are not fully understood. A major conundrum is the lack of association between the severity of inflammatory immune cell infiltration within the salivary glands and glandular hypofunction. In this study, we investigated in a mouse model system, mechanisms of glandular hypofunction caused by the activation of the stimulator of interferon genes (STING) pathway. Glandular hypofunction and SS-like disease were induced by treatment with 5,6-Dimethyl-9-oxo-9H-xanthene-4-acetic acid (DMXAA), a small molecule agonist of murine STING. Contrary to our expectations, despite a significant reduction in fluid secretion in DMXAA-treated mice, in vivo imaging demonstrated that neural stimulation resulted in greatly enhanced spatially averaged cytosolic Ca2+ levels. Notably, however, the spatiotemporal characteristics of the Ca2+ signals were altered to signals that propagated throughout the entire cytoplasm as opposed to largely apically confined Ca2+ rises observed without treatment. Despite the augmented Ca2+ signals, muscarinic stimulation resulted in reduced activation of TMEM16a, although there were no changes in channel abundance or absolute sensitivity to Ca2+. However, super-resolution microscopy revealed a disruption in the intimate colocalization of Inositol 1,4,5-trisphosphate receptor Ca2+ release channels in relation to TMEM16a. TMEM16a channel activation was also reduced when intracellular Ca2+ buffering was increased. These data are consistent with altered local coupling between the channels contributing to the reduced activation of TMEM16a. Appropriate Ca2+ signaling is also pivotal for mitochondrial morphology and bioenergetics and secretion is an energetically expensive process. Disrupted mitochondrial morphology, a depolarized mitochondrial membrane potential, and reduced oxygen consumption rate were observed in DMXAA-treated animals compared to control animals. We report that early in SS disease, dysregulated Ca2+ signals lead to decreased fluid secretion and disrupted mitochondrial function contributing to salivary gland hypofunction and likely the progression of SS disease.
Introduction
Saliva plays crucial roles in oral health, including lubricating the mouth, maintaining pH balance, defense against microorganisms, aiding taste, and initiating digestion of macronutrients (1–3). Saliva is produced primarily by three major salivary glands; the submandibular gland (SMG), parotid gland (PG), sublingual gland (SLG), and some minor glands in the lower lip, tongue, and cheek. Saliva is generated in secretory acinar cells, with its content adjusted by ducts before reaching the mouth. The acinar cells are fundamental to the production of the primary salivary secretion (4). The fluid secretion process is driven by the trans-epithelial movement of Cl- across acinar cells. To accomplish vectorial movement of Cl-, acinar cells are polarized such that the basolateral plasma membrane (PM) faces the interstitium and is adjacent to blood vessels, while the apical PM forms a lumen with the distinct PM regions physically segregated by tight-junctional complexes. At the basolateral PM, Cl- are transported into the acinar cell cytoplasm against their electrochemical gradient via the Na+/K+/2Cl- cotransporter, (NKCC1). Following mastication or the experience of the taste and the smell of food, the neurotransmitter, acetylcholine (ACh) is released from parasympathetic nerves and acts on muscarinic receptors on the basolateral PM. Activated muscarinic receptors promote the production of inositol 1,4,5 trisphosphate (IP3), and subsequently Ca2+ release from endoplasmic reticulum (ER) stores via IP3 receptors (IP3Rs) situated in the ER in the extreme apical aspects of the cell (5–7). Elevated [Ca2+]i activates a Ca2+-activated Cl- channel named TMEM16a that allows Cl- to move through the apical PM to the ductal lumen which is continuous with the salivary intercalated duct (3,8). In turn, Na+ moves through the paracellular space to balance the Cl- and water follows osmotically both paracellularly and through the water channel aquaporin5 (AQP5) to generate the primary saliva(8).
The importance of saliva is underappreciated in the absence of hypofunction. Reduced salivary secretion is termed xerostomia and can result from the iatrogenic effects of drugs, as collateral damage to salivary glands following radiotherapy for malignancy in the head and neck area, and commonly in Sjögren’s syndrome (SS) (9). SS is a chronic autoimmune disorder, that is predominantly manifested as profound dry eye and dry mouth as ultimately the immune system targets and destroys lacrimal and salivary gland cells (10–13). SS can occur independently (primary SS, pSS) or concurrently with diseases such as arthritis or lupus (secondary SS, sSS) (14). SS affects millions of people, predominantly females in their fourth and fifth decades of life. While treatments can alleviate symptoms, there is no cure or intervention to halt its progression. The etiology of SS remains largely unresolved, but it’s believed to result from a combination of genetic, environmental, hormonal, and possibly viral factors, causing an aberrant immune response directed against the exocrine glands. The identification of SS usually is scored by the extent of salivary hypofunction, the degree of immune infiltration, evidence of damage to minor salivary glands observed following biopsy, and the presence of autoantibodies, such as anti-SSA (Ro) and Anti-SSB (La) and anti-nuclear antibody (ANA) which are classically found in SS (12,13,15). Notably, however, in the early phases of SS, there is minor immune infiltration and little overt damage to exocrine tissue despite profound hypofunction. Provocatively, these data indicate that loss of secretory tissue per se is not the causative event resulting in dryness early in the disease, and further indicates that a defect in the stimulus-secretion coupling mechanism precedes glandular destruction and possibly contributes to the progression of the disease.
Over the years, numerous mouse models both genetic and “induced” have been developed to study the pathogenesis of SS, with each exhibiting specific aspects of the human condition, including glandular dysfunction, autoantibody production, and lymphocytic infiltration (16,17). To investigate the early events in SS, we concentrated on an SS model induced by the activation of the stimulator of the interferon gene (STING) pathway. This is thought to mirror the molecular response to bacterial/viral infection. STING is primarily located in the endoplasmic reticulum (ER) and plays a crucial role in the innate immune response, especially against DNA viruses and intracellular bacteria. Activation of STING occurs upon sensing cytosolic DNA as a result of cell damage or from microbial origin following infection. When cytosolic DNA is detected, it is first recognized by a sensor molecule called cGAS (cyclic GMP-AMP synthase). Binding to DNA prompts cGAS to generate cGAMP (cyclic GMP-AMP), which, in turn, binds to and activates STING (18). Once STING is activated, it undergoes a series of transformations that ultimately result in the transcription of type I interferon genes, especially interferon-β (IFN-β) (19). The production of type I interferons is a primary antiviral response and a significant characteristic of SS (19,20). STING can be activated pharmacologically by exposure to 5,6-Dimethyl-9-oxo-9H-xanthene-4-acetic acid (DMXAA), which faithfully reproduces the immune response observed following physiological STING activation (21–23).
In this study, we investigated the early events in the initiation of SS-like disease that lead to salivary gland hypo-function using the DMXAA SS model. We first utilized in vivo intravital imaging to investigate any potential dysregulation of Ca2+ signaling in the DMXAA-induced SS mouse model. Paradoxically, the spatially averaged Ca2+ levels achieved following neural stimulation in mice treated with DMXAA were enhanced despite significantly reduced fluid secretion. Notably, however, the stereotypical spatial characteristics of the Ca2+ signal were disrupted. Downstream of the Ca2+ signal, the activity of the TMEM16a Ca2+-activated Cl channel stimulated by muscarinic secretagogues was reduced, despite no changes in the abundance or localization of the protein or absolute sensitivity to activation by Ca2+. The intimate localization of IP3R and TMEM16a was however disrupted, contributing to the reduced activity of TMEM16a upon agonist stimulation as local peripheral coupling between channels is disrupted. Moreover, we observed disordered mitochondrial morphology, abundance, and function in the disease model. These data suggest that early in SS, reduced fluid secretion occurs because of a defect in the secretagogue activation of Cl- secretion. Further significant mitochondrial dysfunction is evident, possibly as a result of the aberrant Ca2+ signals that may contribute to the dysregulated Ca2+ signals and/or progression of SS disease.
Results
Saliva secretion is attenuated in both SMG and PG in the SS mouse model
Activation of the STING pathway in mice has been established as a model for the initiation of SS. This pathway is normally activated following exposure to foreign nucleic acids and is thought to mimic exposure of cells to DNA/RNA from viruses and bacteria. Activation of this pathway in salivary glands is characterized by initiation of a type-1 interferon response, mild immune cell infiltration, and a marked loss of saliva secretion without obvious morphological damage and therefore mimics the early clinical manifestations of SS disease. Thus, to investigate the early cellular events in acinar cells during the initiation of SS in mice, we chose to pharmacologically activate this pathway using DMXAA, a STING pathway agonist. As described in Methods, DMXAA (or control solution) was administered on day 0 and day 21 of the experimental timeline (Figure 1A). Immunofluorescent staining in sliced SMG tissue indicated that STING protein was increased in SMG in the DMXAA-treated mouse on day 28, seven days after the final DMXAA administration (supplementary Figure 1) confirming the activation of the STING pathway. Whole saliva production from the major salivary glands was evaluated on day 28 following systemic stimulation with the muscarinic receptor agonist, pilocarpine. To avoid potential weight-related variations in saliva secretion, the total saliva output was normalized to the individual mouse’s body weight. Notably, the average saliva production was reduced from 130.1± 48.96 mg in vehicle-treated mice to 63.71± 30.41 mg in DMXAA-treated mice, a reduction in saliva production of 48.97% (Figure 1B). Consequently, DMXAA treatment resulted in 51.99% saliva production compared to vehicle-treated mice (Figure 1C). Moreover, H&E staining indicated that mild immune infiltration was observed in the DMXAA-treated mice with no overt changes to the morphology of the gland (Figure 1D). The mass of the SMG was also not significantly different in DMXAA vs. vehicle-treated animals, consistent with no loss of secretory tissue (Figure 1E). Collectively, these results suggest that DMXAA-treated mice exhibit characteristics of early-stage SS and could be a useful model for investigating the pathophysiological mechanisms underlying secretory dysfunction and advancing our understanding of the disease’s progression.
To further investigate the individual relative contribution of the SMG and PG to the decrease in total saliva secretion using a more physiological stimulation paradigm, we performed experiments where the nerve bundle innervating a particular gland was electrically stimulated and saliva secretion quantitated. Previous research in our lab has established the range and parameters for physiological stimulation of secretion (24). The production of saliva was significantly diminished in DMXAA-treated animals compared with vehicle controls at stimulation frequencies of 7 and 10 Hz in SMG (Figure 1F) and at 5, 7, and 10 Hz in the PG (Figure 1G). These findings confirm that activation of the STING pathway reduces the function of both SMG and PG, consistent with the diminished production of whole saliva. Notably, the reduction in function of PG, the gland responsible for the majority of stimulated saliva secretion, was relatively greater than in SMG.
Altered spatiotemporal characteristics of Ca2+ signals in the SS mouse model
An increase in intracellular Ca2+ plays a central role in regulating the cellular machinery underlying the fluid secretion mechanism. In particular, as noted, an increase in Ca2+ is important for the activation of ion channels localized in particular domains of the polarized acinar cell which play a central role in saliva secretion (24). Given that the precise spatiotemporal characteristics of the Ca2+ signal in salivary acinar cells are thought to be fundamental to the appropriate activation of the fluid secretion machinery, we evaluated whether hypofunction following DMXAA treatment resulted from dysregulation of the stimulated Ca2+ signal. Previous research in our lab developed a platform to study Ca2+ signaling in vivo using Multiphoton (MP) imaging in transgenic mice engineered to express a genetic-encoded Ca2+ indicator, GCaMP6f, specifically in acinar cells (24). The protocol for STING pathway induction was applied to the Mist1CreERT+/-GCaMP6f+/- genetic mouse (supplementary Figure 2A). Salivary gland function was assessed by the amount of pilocarpine-induced saliva secretion. The secretion deficiency observed in wild-type mice was recapitulated in these mice from a different genetic background (supplementary Figure 2B). We reasoned that decreased fluid secretion could result from reduced or dysregulated Ca2+ signaling in DMXAA-treated mice. Therefore, next, we compared the spatially averaged Ca2+ signal evoked by direct nerve stimulation in DMXAA-treated vs. vehicle control animals in vivo in SMG. SMG were stimulated at frequencies optimum for fluid secretion (1-10 Hz) for 10 seconds and the Ca2+ signals were recorded. Figure 2A are standard deviation (SD)-projection images visualizing the spatial and amplitude changes in Ca2+ throughout the field of view during the entire period of stimulation at the indicated frequencies. In vehicle-treated mice, the Ca2+ signals at low stimulus strengths occurred in a minority of the cells and predominantly propagated below the apical PM. As the stimulation strength increased, Ca2+ signals became more pronounced, and more acinar cells responded. Strikingly, acinar cells in DMXAA-treated mice demonstrated enhanced sensitivity to stimulation. At lower stimulation strengths, a larger number of acinar cells responded, and the spatially averaged Ca2+ signals in these cells were notably larger when compared to those in the control group. Figure 2B shows a time series of images following 7 Hz stimulation. This augmented response was manifested as an elevated maximum peak [Ca2+] (Figure 2D), shorter latency (Figure 2E), and larger area under the curve (AUC) during stimulation in DMXAA-treated animals (Figure 2F).
In addition to the absolute magnitude of the spatially averaged Ca2+ signal, the subcellular spatial characteristics of the stimulated Ca2+ rise are also important for appropriate stimulation of fluid secretion (24). Ca2+ signals stimulated following nervous stimulation in SMG are invariably initiated in the extreme apical pole of acinar cells and subsequently establish a standing gradient that dissipates rapidly to result in apically confined signals that do not substantially propagate to the basal aspects of the cell following physiological stimulation (24). We therefore investigated if the spatial characteristics of stimulated Ca2+ signals were altered in DMXAA-treated animals. SD image projections generated during the period of stimulation demonstrated that the [Ca2+]i increase was tightly localized below the apical PM within the acinar cells in the vehicle-treated animals (Figure 3A). However, in the DMXAA-treated animals, the [Ca2+]i exhibited a more global distribution through the entire cell cytoplasm (Figure 3B). The [Ca2+]i was visualized via line-scan plots revealing the temporal alterations along a line extending from the apical PM to the basolateral PM, traversing the nucleus, over time within an acinar cell. A significant [Ca2+]i elevation was evident at the basolateral aspects of the acinar cell in the DMXAA-treated animals (Figure 3D) when compared to the vehicle-treated control (Figure 3C). As shown in the kinetic plots, the [Ca2+]i in the apical region is greater in DMXAA-treated mice compared to the vehicle-treated mice (Figure 3E). Moreover, a significant Ca2+ signal was observed in the extreme basal region of the cell in DMXAA but not in vehicle-treated animals (Figure 3F). The comparison of Ca2+ signal ratios at the apical versus basolateral PM indicated the most significant globalization of the Ca2+ signal occurred at 10 Hz stimulation (Figure 3G) which corresponds to the stimulation strength that results in maximal fluid secretion (24). In total, these data demonstrate that the magnitude of the spatially averaged Ca2+ signal, together with the spatiotemporal characteristics of the signal are altered in the DMXAA-treated animals, but that these changes can not readily account for the reduction in fluid secretion observed.
Secretagogue stimulated TMEM16a activity is suppressed in the SS mouse model
The rate-limiting step for the secretion of fluid is the activation of the Ca2+-activated Cl- channel, TMEM16a. We considered that a reduction in fluid secretion could conceptually occur by a reduction or mislocalization of TMEM16a protein, or by compromised muscarinic receptor-stimulated activation of the channel. Western blotting indicated the TMEM16a protein expression was comparable between the vehicle and SS mouse models (Figure 4A and 4B). In addition, immunolocalization using confocal microscopy demonstrated that TMEM16a localization remained largely unchanged in the SS mouse model (Figure 4C). Thus, a decrease in protein expression or mislocalization of the protein does not result in a reduction in stimulated saliva secretion. We next investigated whether the activation of this ion channel was compromised in DMXAA-treated animals using whole-cell patch clamp electrophysiology. In the absence of stimulation, no Cl- currents were observed in either vehicle or DMXAA animals following either depolarizing or hyperpolarizing voltage steps from a holding potential of −50 mV (Figure 4D). In the presence of 1 μM of muscarinic agonist Carbachol (CCh), robust Cl- currents were measured in acini prepared from vehicle-treated animals (Figure 4E), which were greatly reduced in DMXAA-treated animals (Figure 4D and 4F). Reduced CCh-stimulated Cl- currents could potentially occur because of altered Ca2+ regulation of TMEM16a following disruption of the spatial characteristics of the stimulated Ca2+ signal. Theoretically, it is also possible that the [Ca2+]i in the immediate vicinity of TMEM16a was disrupted, despite the augmented spatially averaged peak response. We therefore next tested whether TMEM16a activity stimulated directly by 0.5, 1 or 5 μM Ca2+ in the pipette solution (and thus globally in the cytoplasm) was altered in DMXAA-treated animals. Surprisingly, TMEM16a was activated to a similar extent by Ca2+ in the SS mouse model (Figure 5A and 5B). In total, our data suggest that TMEM16a abundance, localization, or activity per se are not altered and thus do not explain the significant reduction in saliva secretion in the DMXAA-treated model.
An alternative mechanism could be that the microdomain between the apical ER Ca2+ release sites and the apical PM TMEM16a is disrupted in the disease model, resulting in compromised local coupling between the ER and PM channels. Therefore, we investigated how the activation TMEM16a was affected by buffering the CCh-stimulated cytosolic Ca2+ with slow and fast Ca2+ chelators. EGTA is a high-affinity Ca2+ buffer with slow kinetics, while BAPTA has much more rapid kinetics. Experimentally, BAPTA can quickly buffer Ca2+ changes close to Ca2+ release sites and limit local activation of effectors within 20 nm. In contrast, EGTA has been shown to attenuate the rise in the bulk cytosol but is too slow to buffer local Ca2+ in a restricted microdomain (25). In the presence of BAPTA, no CI- currents were detected in either vehicle-treated or DMXAA-treated animals (Figure 6A). However, in the presence of the slow Ca2+ chelator, EGTA, CCH-stimulated Cl- currents were observed in acinar cells from vehicle-treated animals but were absent in the DMXAA-treated animals (Figure 6A and 6B). These data suggest that physiologically TMEM16a is activated by local changes in Ca2+ signaling as a result of IP3-induced Ca2+ release in the apical domain of the acinar cell.
Next, we employed STED super-resolution microscopy to closely examine the spatial relationship between apical PM TMEM16a and IP3R3 on the apical ER (Figure 7A). Despite the cell-cell contact distance remaining consistent in the disease model, as indicated by the distance between TMEM16a on the PM of adjacent acinar cells (Figure 7F), a notable increase in distance between the apical TMEM16a and IP3R3 expressed on apical ER compared to the control group was observed (Figure 7E). In the control mice, the distance between TMEM16a and IP3R3 was on average 84 ± 17 nm, versus 155 ± 20 nm in the SS disease mice. Similarly, the distance between IP3R3 in adjacent cells was increased from 505 ± 34 nm to 689 ± 68 nm (Figure 7C and 7D). In total, these observations support the conclusion that the reduced activity of the TMEM16a channel is attributable to the disruption of the microdomain between TMEM16a and IP3R3, such that the Ca2+ flux through the IP3R is not communicated appropriately to its effector, TMEM16a.
Compromised mitochondrial morphology and metabolism in the SS mouse model
Ca2+ modulates cellular metabolism by the intricate bidirectional interaction between the ER and mitochondria. Ca2+ transfer between ER and mitochondria is essential for optimal bioenergetics, and dysregulated [Ca2+]i can be deleterious to mitochondrial function and alter morphology (26–29). The transfer of Ca2+ between ER and mitochondria is dependent on the intimate physical localization of the organelles (29). Notably, aberrant mitochondrial morphology has been reported in the salivary glands of SS patients (30). We first investigated mitochondrial abundance and morphology by immunofluorescence staining with antibodies directed against ATP5A, a component of the ATP synthesis machinery to visualize mitochondria, and Na+/K+ ATPase to localize the plasma membrane (Figure 8A). Using previously published methodologies (31,32), quantification revealed a 22.16% ± 4.95 reduction in mitochondrial numbers in the SS mouse model relative to the vehicle-treated control (Figure 8B). Consistent with reduced mitochondrial numbers, less area was occupied by mitochondria in DMXAA-treated acinar cells (Figure 8C). Mitochondria morphology is intricately linked to their bioenergetic status We next evaluated mitochondrial morphology by their “so-called” aspect ratio (AR) and form factor (FF) in DMXAA and vehicle-treated animals. The AR, the length of the major over minor axes of mitochondria documents the degree of fragmentation or elongation of individual mitochondria (29,32). Mitochondria exhibited an 18.35% ± 4.62 decrease in mitochondrial elongation (Figure 8D) and a 20.7% ± 7.78 decrease in mitochondrial branching (Figure 7E) in the disease model compared to the vehicle-treated control condition. Importantly, these changes in mitochondrial number and morphology were not exclusive to the SMG as similar patterns were observed in the PG mitochondria, again marked by reduced mitochondrial count, increased fragmentation, and decreased branching (supplementary Figure 3A-3E).
Next, we utilized electron microscopy (EM) to investigate mitochondrial ultrastructure. At low magnification, acinar cells from control mice contained defined mitochondria and well-formed ER stacks (Figure 9A. blue arrow). In contrast, the ER structure was disrupted in the SS disease model (Figure 9A’). At higher magnification, the coordinated ER structure was largely absent in diseased mice (Figure 9B and 9B’), and the proximity between ER and mitochondria was disrupted (Figure 9H and 9I). Moreover, we also observed scattered mitochondrial cristae at the highest magnification (Figure 9C’ and 9G). Consistent with immunofluorescence studies, quantification of EM micrographs revealed that mitochondria were smaller, more fragmented (Figure 9D and 9E), and rounder (Figure 9F) in shape. In summary, our results collectively indicate significant morphological alterations in mitochondria in the SS disease model.
Mitochondrial morphology is a dynamic process that is intimately associated with mitochondrial bioenergetics and alterations in both, occur in response to changes in cellular status (33–36).Therefore, we investigated if changes in morphology might be associated with the disrupted function of mitochondria in the disease model. We measured mitochondrial membrane potential (ΔΨm), established by the electrochemical H+ gradient, which is the driving force of ATP production. Isolated SMG acinar cells were loaded with TMRE, a ΔΨm-specific dye, and MitoTracker Green, to confirm mitochondrial localization and to facilitate the normalization of indicator loading. The maximal z-stacks projection images taken by confocal microscopy revealed colocalization of TMRE with MitoTracker Green (Figure 10A). The basal TMRE fluorescence was reduced in cells from DMXAA vs. vehicle-treated animals (supplemental Figure 4). To assess ΔΨm, we quantified the relative maximum dissipation of ΔΨm in DMXAA and vehicle-treated acinar cells by the mitochondrial uncoupler, FCCP (Figure 10B). Consistent with the reduction in basal TMRE fluorescence, the change in TMRE fluorescence normalized to mitochondrial content revealed a marked reduction in ΔΨm in the acinar cells from the SS disease model (Figure 10C).
An appropriate ΔΨm mitochondrial membrane potential is vital for maintaining bioenergetics (37). Given that mitochondrial ΔΨm was significantly depolarized in DMXAA-treated animals, we next evaluated the OCR, a key metric of mitochondrial bioenergetic function in isolated SMG acinar cells. We employed sequential exposure to agents that target the function of the mitochondrial electron transport chain (ETC) using Seahorse technology (Figure 10D). Our results revealed a 25% reduction in basal OCR in the SS model compared to the control animals (at −25.25 ± 7.89 pmol/min; Figure 10E). While ATP-linked respiration showed no significant difference in post-oligomycin-induced ETC Complex V blockade in both conditions (Figure 10F). Intriguingly, the FCCP-provoked maximal respiration rate, an indicator of stress tolerance, remarkably declined by 47% ± 9.19 after FCCP treatment in the SS model (Figure 10G). These data indicate impaired mitochondrial function and stress responses in the SS mouse model.
Discussion
SS is a complex inflammatory disease resulting from the intersection of genetics and environmental factors. This autoimmune disorder affects exocrine glands including salivary and lacrimal glands, leading to dry mouth and dry eyes, among other symptoms (11–13). SS animal models are crucial for understanding the pathogenesis, progression, and potential treatments for the disease, though like many animal models of disease, none can recapitulate all the aspects of SS. Currently, SS animal models are categorized as either those derived from genetically modified mice (14,38–40) or those where disease is induced by specific agents or environmental factors. In the context of SS, DMXAA-induced SS can be used to mimic the early stages of the disease which might be triggered in response to bacterial or viral infection. This model is particularly effective in simulating type-1 interferon immune responses seen in early SS, which is thought to contribute to the initial glandular inflammation (10,19,41). It should also be noted that DMXAA also has been reported to inhibit NAD(P)H quinone oxireductase (42) and thus the potential increase in free radical load in cells could contribute to the phenotype. The rapid symptom manifestation of disease in the DMXAA-induced model offers an advantage for investigating the early development of SS disease since DMXAA induction is a temporally controlled process, allowing the precise staging of disease onset, thus facilitating studies on the initiating events and ultimately potential early intervention and prevention strategies.
Our studies investigated stimulus-secretion coupling when fluid secretion from SMG and PG in response to physiological stimulation was significantly reduced. Previous work has established a crucial link between an increase in [Ca2+]i and stimulation of fluid secretion in the salivary glands (8,24). Efficient secretion is reliant on the specific spatiotemporal regulation of secretagogue-stimulated [Ca2+]i signals. Given this idea, our initial hypothesis was that a deficiency in secretion after DMXAA administration could be due to reduced or disrupted secretagogue-stimulated [Ca2+]i signals. Indeed, previous work has revealed that in human SS patient acinar cells and the IL14α knock-in transgenic SS mouse model, CCh-induced [Ca2+] signals were diminished. This reduction was attributed to lower expression levels of the IP3R2 and IP3R3 proteins (43). To probe this hypothesis, we employed transgenic Mist1CreERT2+/– x GCaMP6f+/– that expresses Ca2+ indicator-GCaMP6f specifically in the acinar cells. Firstly, we validated that the activation of the STING pathway leads to similar salivary gland hypofunction in this genetic background (supplementary Figure 2). Surprisingly, however, DMXAA treatment led to a striking increase in the magnitude of neurally-induced spatially averaged [Ca2+]i signals. This observation is not consistent with the loss of IP3R proteins being responsible for reduced fluid secretion previously reported in other SS models. Indeed, the expression of IP3R proteins was unchanged following DMXAA treatment (supplementary Figure 6). The discrepancy could be attributed to the stage of SS disease represented by the previous studies, with our data presenting an earlier initiating phase of SS disease prior to progression, at a time point before any notable decrease in IP3R proteins has occurred. The molecular mechanism responsible for augmented global Ca2+ signals following DMXAA treatment requires further study. Increased Ca2+ release/influx, or conversely reduced Ca2+ clearance might be responsible. It is tempting to speculate that reduced mitochondrial Ca2+ uptake and/or reduced PMCA and SERCA activity as a result of decreased ATP levels may contribute to enhanced cytosolic signals. Nevertheless, we suggest that the augmented Ca2+ signals might represent a compensatory mechanism to drive fluid secretion in the face of compromised physiological stimulus-secretion coupling. Although the Ca2+ signals were not reduced, the spatiotemporal characteristics of the Ca2+ signal were markedly disrupted. Specifically, during neural stimulation, while in control animals there is a pronounced standing gradient of [Ca2+] such that the [Ca2+] is much greater in the apical vs. basal aspects of the cell, in DMXAA-treated animals this gradient is largely absent as large changes in Ca2+ are propagated to the basal regions of the cells. It is conceivable that the alteration in magnitude coupled with changes in the spatial characteristics of the Ca2+ signal contributes to both the defect in fluid secretion and downstream cellular changes including mitochondrial damage to ultimately result in the progression of disease.
We investigated whether changes in the secretory machinery per se were altered in DMXAA-treated animals to result in hyposecretion. Salivary gland fluid secretion is dependent on TMEM16a facilitating CI- flux across the apical PM as the driving force for water transport paracellularly and through AGP5 (44,45). The loss of either TMEM16a or AQP5 results in markedly attenuated fluid secretion (45–48). These findings indicate that alteration in expression level, localization, or regulation of these channels could potentially impact fluid secretion. Notably, in DMXAA-treated mice, the AQP5 expression and localization remain unchanged (supplementary Figure 5), consistent with a study in human labial minor salivary glands (49). We next examined whether the TMEM16a channel function was compromised in the model. Our electrophysiological analysis revealed a significant decrease in TMEM16a activity following CCh-induced stimulation. Again, this reduced activity was not the result of overt mislocalization or lower expression levels of the protein (Figure 4A and 4C). Interestingly, although the secretagogue-stimulated TMEM16a was reduced in acinar cells from DMXAA-treated animals, the sensitivity of the channel to direct activation by Ca2+ in the patch pipette appeared unaffected. IP3R3 Ca2+ release channels on the ER are located approximately 50-100 nm from TMEM16a on the PM (7). In this microdomain, confocal microscopy cannot easily distinguish the distinct localization of TMEM16a/IP3R, despite their localization on different membranes. However, STED super-resolution microscopy provides a much higher spatial resolution, achieving 20-80 nm to enable the differentiation of proteins within 20-80 nm of each other. Data using STED microscopy, suggest that the microdomain between apical ER IP3R3 and apical PM TMEM16a is disrupted in the disease model. The severe fragmentation of ER observed in EM images from DMXAA-treated animals also is consistent with an alteration in the relationship between ER and other intracellular domains. The disruption of the relative localization of these channels could conceivably result in diminished TMEM16a activity, if activation is dependent on the local [Ca2+] in its vicinity. Our data showing that the slow Ca2+ buffer EGTA eliminates TMEM16a activation in the disease model, but that currents can still be evoked in vehicle-treated controls is consistent with the activation of TMEM16a by the local Ca2+ signal surrounding the channel rather than the global cytoplasmic Ca2+ signal (44,50,51). Thus, the disruption of this apical microdomain likely alters the local Ca2+ signal that TMEM16a experiences leading to reduced activation and fluid secretion.
While changes in cytosolic [Ca2+] are vitally important for stimulating ion flux and hence fluid secretion, Ca2+ is also critical for numerous other physiological processes in salivary gland acinar cells. We focused on the potential effects of the dysregulated Ca2+ signal on mitochondrial morphology and function. Secretion is an energy-demanding process, necessitating a constant supply of ATP for numerous functions, including vesicle transport, protein modification, membrane fusion, and maintaining ion gradients. For example, the Na+/K+ ATPase pump generates the Na+ gradient, driving Cl- transport into the cytosol of acinar cells through NKCC1, and SERCA pumps replenish ER Ca2+ levels. In this context, mitochondria are essential as they provide ATP, regulate Ca2+ homeostasis, supply metabolic intermediates, and coordinate with the ER to orchestrate cellular functions (52,53). Notably, recent studies have highlighted that mitochondria are abundant and display varied positioning and dynamics in salivary gland cells (54). In SS patients, there are notable alterations in mitochondrial structure, including swelling and disrupted cristae (30,55). Correspondingly, mitochondrial-related genes, particularly those involved in metabolism, dynamics, and the electron transport complex, are significantly affected (55). Our data, employing fluorescent immunostaining and EM, mirrors these findings in DMXAA-treated animals. We observed that mitochondrial morphology is altered such that mitochondria are more swollen and rounded, with dispersed cristae, similar to that reported in human SS patients (30). Since optimal mitochondrial bioenergetics are also dependent on Ca2+ signals, we assessed mitochondrial function by measuring the mitochondrial membrane potential (ΔΨm) using a membrane potential sensitive probe and the OCR using Seahorse technology. Our results show that in the SS mouse model, ΔΨm, which is critical for ATP synthesis, is diminished (Figure 10C and supplementary Figure 4). While the ATP-linked OCR remained unchanged, both the basal and maximal OCR were reduced. This suggests that mitochondrial functionality is compromised in the disease model, indicating a decreased capacity to respond to additional cellular stress. An intriguing question arises from these findings: are defects in the function of mitochondria a primary cause of fluid secretion loss in SS, or alternatively is this a consequence of disrupted [Ca2+]i regulation? Moreover, DNA from damaged mitochondria can activate the cGAS/STING pathway, leading to inflammation(18,21). This implies that compromised mitochondria in early SS stages could trigger prolonged inflammation through the STING pathway, potentially contributing to SS progression. Understanding these mechanisms is crucial for developing effective treatments to halt or slow the progression of SS.
Material and Methods
All animal procedures were approved by the University of Rochester Committee on Animal Resources (UCAR-2001-214E)
Animals
The murine model of Sjögren’s syndrome was established through the induction of the STING pathway (56). Briefly, 8-10 weeks old female C57BL/6J wild type (WT) mice (Jackson Laboratory; Jax 000664) received subcutaneous injections of DMXAA (Vadimezan; GC16280) at a concentration of 25 mg/kg of body weight on both day 0 and day 21 of the experimental timeline (see figure 1). The control mouse received vehicle (5% sodium bicarbonate; Sigma-Aldrich; S8761), the DMXAA solvent at the corresponding time points. Experiments were performed on day 28 of the experimental timeline.
Evaluation of saliva production
The mice were fasted for two hours prior to the evaluation of saliva production. The mice were anesthetized with a solution containing Ketamine (10 mg/mL) and Xylazine (1 mg/ml) by intraperitoneal injection (IP) at a dose of 7 μl/gm body weight over 2 minutes. The mouse was placed on a heating pad at 37℃ during experimentation. A Salimetrics Childen’s swab (Salimetrics; Cat. no. 5001.05) was placed within the oral cavity of each mouse. The mice were administered the muscarinic agonist pilocarpine (0.375 mg/kg body weight; Millipore Sigma; P6503) by IP injection. Two minutes after the pilocarpine injection, saliva was collected for the following 15 minutes. The saliva absorbed was subsequently separated from the moist swab through centrifugation at 10,000 rpm for 1 minute. The measurement of saliva weight served as a quantitative evaluation of the efficacy of whole saliva secretion. To measure neurotransmitter-stimulated saliva secretion more directly, the mouse was anesthetized as previously described (24) and a surgical incision was made in the skin to expose the submandibular gland (SMG). The surrounding connective tissue was excised to facilitate positioning within a custom-made 3D-printed gland holder. A pair of stimulation electrodes were attached to the duct bundle and the SMG. The pre-weighed filter paper was positioned within the oral cavity of the mouse to capture saliva secretion. Secretion was initiated by electrical stimulation sequences generated by a stimulus isolator (Iso-flex, A.M.P.I.) set at 5 mA, 200 ms, at frequencies of 1, 3, 5, 7, and 10 Hz with train frequency and duration (typically 1 minute) controlled by a train generator (DG2A, Warner Instruments). The interval between each stimulus was 3 minutes. After stimulation, the filter paper was removed and weighed. The difference between the weight of filter paper before and after the electrode stimulation represented the saliva produced by the respective salivary gland during the given stimulation period.
In vivo Ca2+ imaging
Mist1CreERT2+/– x GCaMP6f+/– transgenic mice served as the experimental subjects for Ca2+ imaging of the submandibular gland (SMG) in vivo. The generation of Mist1CreERT2+/– x GCaMP6f+/–transgenic mice by crossing GcaMP6fflox mice (Jackson Laboratory; Jax 028865) with Mist1CreERT2 (Jackson Laboratory; Jax 029228, a gift from Dr. Catherine Ovitt, University of Rochester). A week before the DMXAA or 5% sodium bicarbonate injections, tamoxifen (Sigma-Aldrich; T5648) was given to the mice via oral gavage at a dose of 0.25 mg/g of body weight for 3 consecutive days to excise the loxP sites flanking the STOP codon allowing expression of the Ca2+ indicator within salivary glands. The mice were anesthetized and gland-exposed, as described previously (24,57). The immobilized gland was secured within the holder using a cover glass and maintained in Hank’s salt solution (HBSS). Ca2+ imaging was conducted in vivo via two-photon microscopy using an Olympus FVMPE-RS system equipped with an Insight X3 pulsed laser (Spectra-Physics) utilizing a heated (OKOLab COL2532) 25x water immersion lens (Olympus XLPlan N 1.05 W MP). GCaMP6f was excited at 950 nm and emission collected between 495–540 nm, with images captured at 0.5-second intervals following stimulation for 10 seconds with 3 minutes between stimulation periods. Statistical analyses were performed with two-way ANOVA with multiple comparisons using Prism (GraphPad) as indicated in the figure legends.
Immunofluorescent staining for sliced tissue
Following verification of decreased saliva secretion in mice, glands were processed for immunocytochemistry. Briefly, the isolated salivary glands were fixed in 4% paraformaldehyde at 4℃ overnight. The fixed gland was processed, embedded in paraffin, and subsequently sliced into 5 μm thick sections. Two temperature-induced antigen retrieval protocols were used either based on HIER buffer (10 mM Tris-base, 1 mM EDTA-dehydrate, pH 9.2) or sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, pH 6.0). Gland sections were blocked with the 10% donkey serum in 0.2% PBSA (PBS+ BSA) at room temperature (RT) for 1 hour. Sections were incubated with the primary antibody at 4℃ overnight (TMEM16a (Millipore Sigma; P6593; 1:250), Na+/K+ ATPase (Abcam; ab2872; 1:250), ATP5A (Abcam; ab14748; 1:500), AQP5 (Abcam; ab239904; 1:500), STING (Cell signaling Technology, Cat. 13647; 1:500)). Following washing, the sections were then incubated with the secondary antibody at RT for 1 hour (Donkey anti-rabbit Alexa 488 (Thermo Fisher Scientific; A-21206; 1:500), Donkey anti-mouse Alexa 594 (ThermoFisher Scientific; A-21203; 1:500)). Nuclei were identified by incubation in DAPI (Thermo ScientificTM; Cat. 62248; 1:1000) at RT for 5 minutes. Tissue sections were mounted using Immu-Mount solution on a slide and then sealed under a coverslip. Images were acquired by Olympus FV1000MP confocal microscopy employing an Olympus UPlanSApo 60x oil immersion objective. The analysis of images was performed using FIJI software. Statistical analyses were performed with a t-test using Prism (GraphPad) as indicated in the figure legends.
Patch clamp electrophysiology
Acinar cells were allowed to adhere to Cell-Tak-coated glass coverslips for 15 minutes before experimentation. Coverslips were transferred to a chamber containing extracellular bath solution (155 mM tetraethylammonium chloride to block K+ channels, 2 mM CaCl2, 1 mM MgCl2, 10 mM HEPES, pH 7.2). Cl- currents in individual cells were measured in the whole cell patch clamp configuration using pClamp 9 and an Axopatch 200B amplifier (Molecular Devices). Recordings were sampled at 2 kHz and filtered at 1 kHz. Pipette resistances were 3–5 MΩ, and seal resistances were greater than 1 GΩ. Pipette solutions (pH 7.2) contained 60 mM tetraethylammonium chloride, 90 mM tetraethylammonium glutamate, 10 mM HEPES, 1 mM HEDTA (N-(2-hydroxyethyl) ethylenediamine-N, N’, N’-triacetic acid) and 20 μM CaCl2 were used to mimic physiological buffering and basal [Ca2+]i conditions (∼100 nM Ca2+). Free [Ca2+] was estimated using Maxchelator freeware. Agonists were directly perfused onto individual cells using a multibarrel perfusion pipette. The pipette solution for the increased basal [Ca2+]i contained hEDTA and a free [Ca2+]i of 500 nM, 1 μM, or 5 μM to induce calcium-activated Cl- currents without the addition of any agonists. Experiments comparing EGTA, BAPTA, and HEDTA effects upon chloride currents induced by CCh stimulation contained 5 mM free concentrations of the chelator and 100 nM free [Ca2+] in the patch pipette. Chloride currents following agonist application were monitored with a single voltage step to 80 mV from a holding potential of −80 mV every second until current magnitudes reached a plateau. Current-voltage relationships were obtained by 20 mV incremental steps between −80 mv and 120 mV from a holding potential of −50 mV.
STED microscopy
3D STED microscopy was performed using an Abberior Instruments Expert Line STED microscope equipped with an Olympus UPLSAPO ×100/1.4NA oil immersion objective. Briefly, lobules <1 mm were isolated following injection of saline beneath the capsule with a 29-gauge needle. The connecting tissue was digested in 0.1 mg/ml collagenase containing image buffer at 37℃ for 5 minutes. Then isolated lobules were fixed in 100% methanone at −20℃ for 5 minutes, and subsequently were blocked with 10% BSA in 0.1% PBST (PBS+ 0.1% Tween20) at RT for 1 hour with gentle shaking. Isolated lobules were incubated with primary antibodies overnight at 4°C (TMEM16a (Millipore Sigma; P6593; 1:300), IP3R3 (BD Transduction Laboratory; Cat. 610313; 1:200)). After being washed with 0.1% PBST, the membranes were incubated with secondary antibodies at RT for 1 hour (STAR RED, goat anti-rabbit IgG secondary antibody (Abberior, Cat#STRED-1001-500UG; 1:1000), Alexa Fluor 594 anti-rabbit IgG secondary antibody (Molecular Probes Cat#A-11037; 1:1000)). The tissue was mounted on the slides with ProlongTM Gold antifade reagent (Invitrogen; Cat. P36930. Sequential confocal and STED images were obtained following excitation of Alexa Fluor 594 and STAR RED by 594 and 640 nm lasers, respectively. Both fluorophores were depleted in three dimensions with a 775 nm pulsed STED laser. Z-stacks were obtained by collecting images at 50 nm intervals using the 3D STED mode. Rescue STED was employed to minimize the light dosage. Blend mode depth projection images were generated and fluorophore volumes and interfaces between these volumes were analyzed using FIJI.
Seahorse XF cell mito stress assay
Isolated SMGs were finely minced and subsequently resuspended in a solution composed of 0.5% Bovine Serum Albumin (BSA) in Hank’s Balanced Salt Solution (HBSS). To isolate acinar cells, the minced tissue was incubated in 0.5% BSA/HBSS containing 0.2 mg/ml of collagenase type II (Worthington; LS004204) for 30 minutes. Following this incubation, the suspension of cells was centrifuged at 500 rpm for 1 minute and the cellular pellet was then resuspended in 40 μg/ml of Trypsin inhibitor (Millipore; Cat. 65035) to terminate further digestion. The function of mitochondria was assessed in isolated acinar cells by measurement of oxygen consumption rate (OCR) employing a Seahorse XF Cell Mito Stress Test system (Agilent, USA). Briefly, Equal sized SMG cell pellets were suspended in buffer and 10 μl of the acinar cell suspension was seeded into individual wells of Seahorse cell culture microplates coated with 10 uL of Cell-Tak (0.25mg/ml) and the OCR was determined utilizing the Seahorse XFe96 extracellular flux analyzer following sequential exposure to 4μg/ml oligomycin (Millipore Sigma; O4876), 4µM carbonyl cyanide-4 (trifluoromethoxy)phenylhydrazone (FCCP; Millipore Sigma; C2920), and 0.5 µM rotenone/antimycin (Millipore Sigma; R8875; A8674) to measure the quantification of basal respiration, ATP-linked respiration, and maximum respiration rate, respectively. Statistical analyses were performed with, t-test using Prism (GraphPad) as indicated in the figure legends.
Measurement of mitochondrial membrane potential
Isolated SMG acinar cells were loaded with 20 nM Tetramethylrhodamine, Ethyl Ester (TMRE; ThermoFisher Scientific: T669), and 1μM of MitoTracker Green (InvitrogenTM; M7514). Fluorescence of both TMRE and MitoTracker Green was captured simultaneously using an inverted epifluorescence Nikon microscope with a 40x oil immersion objective. The TMRE fluorescence was excited at 560 nm and emitted light collected at 574 nm; MitoTracker Green was excited at 488 nm and emitted light collected at 530 nm. Images were obtained every 1 s with an exposure of 20 ms and 4 x 4 binning using a digital camera controlled by TILL Photonics, TILLvision software. The acinar cells were exposed to 4 μM FCCP for 3 minutes by perfusion to rapidly dissipate the membrane potential. Mitochondrial membrane potential was quantified as the change in the ratio of TMRE/MitoTracker Green fluorescence before and after the administration of FCCP. Statistical analyses were performed with a t-test using Prism (GraphPad) as indicated in the figure legends.
Western blotting
Finely minced salivary glands were homogenized in a lysis buffer supplemented with protease inhibitor cocktail (Complete mini; Roche Diagnostics) for 16-20 strokes. After incubating on ice for 30 minutes, solubilized proteins were separated by centrifugation at 13000 rpm at 4℃ for 30 minutes. 10μg of protein lysate was loaded on 7.5%-12% SDS-polyacrylamide gels. Subsequently, the proteins were transferred to PVDF membranes at a voltage of 35V at 4°C overnight. The membrane was blocked with 5% non-fat skimmed milk in TBST (50 mM Tris-HCl, pH 7.5 with 0.1% Tween20) at RT for 1 hour and subsequently incubated with primary antibodies overnight at 4°C (Actin (Millipore Sigma; A2228; 1:10000), IP3R2 (Antibody Research Corporation; 1:1000), IP3R3 (BD Transduction Laboratory; Cat. 610313; 1:1000), TMEM16a (Abcam; ab84115; 1:1000)). After being washed with 0.1% TBST, the membranes were incubated with secondary antibodies at RT for 1 hour (Goat anti-rabbit IgG (H&L) (Invitrogen; SA535571; 1:10000), Goat anti-mouse IgG (H&L) (Invitrogen; SA535521; 1:10000)). Protein band intensity from western blotting was quantified by FIJI. The relative ratio of DMXAA-treated/ vehicle control was calculated in Excel. Lastly, graphical generation and statistics were performed with a t-test using Prism (GraphPad) as indicated in the figure legends.
Acknowledgements
The authors gratefully acknowledge the University of Rochester’s Center for Advanced Microscopy and Nanoscopy (CALMN) for providing access to Multiphoton microscopy for in vivo live imaging and STED super-resolution microscopy, and for Center for Advanced Research Technologies (CART) for the Electron & cryo Microscopy Resource. We also thank the Flow Cytometry Resource (FCR) for its support with the mitochondrial stress assay. Special thanks to Dr. Paul Brooks for sharing Seahorse Technology XF analyzers and for engaging in discussions for optimization of experiments. Thanks to Dr. Catherine Ovitt for her instruction on tissue staining techniques. Additionally, we wish to express our appreciation to all members of the Yule laboratory for their invaluable feedback, discussions, and assistance, which have been essential in advancing this study. The work was supported by a grant from NIH (NIDCR) DE014756 (to DIY).
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