Abstract
Tendon injury occurs at high frequency and is difficult to repair. Identification of human stem cells being able to regenerate tendon will greatly facilitate the development of regenerative medicine for tendon injury. We identified CD29+/CD56+ human muscle stem/progenitor cells having tendon differentiation potential both in vitro and in vivo. Transplantation of human myogenic progenitor cells contributes to injured tendon repair and thus improves locomotor function. Interestingly, the tendon differentiation potential in mouse muscle stem cells is minimal and the higher TGFβ signaling level in human myogenic progenitor cells may be the key for the distinct feature of human myogenic progenitor cells. These findings reveal that CD29+/CD56+ human muscle stem/progenitor cells are bi-potential adult stem cells and can serve as a new source for tendon regeneration.
Introduction
Skeletal muscle is a tissue with great regeneration ability due to the existence of muscle stem cells (MuSCs). MuSCs are adult stem cells located at the periphery of myofibers between the basal lamina and the plasmalemma of the myofibers and responsible for muscle regeneration1,2. MuSCs have been considered to be unipotent stem cells to have a sole differentiation potential to myofibers3. MuSCs undergo expansion and differentiate to multinuclei myofibers after injury in vivo. MuSCs have remarkable abilities to support muscle regeneration. After transplantation, the engrafted mouse MuSCs go through active expansion and regenerate myofibers4. Isolation by FACS and expansion of MuSCs have been reported from several species including mouse, pig, and human5,6. Single cell sequencing analysis from human skeletal muscles have also revealed the existence of Pax7+ MuSCs6,7. Due to the different motion patterns, the regeneration capacity may be different between human and rodents. Current investigations have suggested that human myogenic progenitor cells didn’t share same markers as that in mice8, and the expression pattern of oxidative enzymes and cytokines between these two species are also different9, suggesting that human muscle progenitor cells may have distinct features from mice.
FACS method to isolate cells with myogenic differentiation potential from human muscle biopsies has been established. CD56 (NCAM)+ and CD56+ CD29+ cells from skeletal muscle display myogenic differentiation potential9,10,11,12. The CD56+ CD34+ progenitor cells isolated from human skeletal muscle biopsies have been reported to have chondrogenic, adipogenic, and osteogenic potentials besides myogenic potential13–15. CD56+CD34-progenitor cells are free of adipogenic potential11. The differentiation potential of human muscle progenitor cells remains to be further explored.
Skeletal muscle directly connects to tendons which is responsible for transmitting forces from skeletal muscle to bone to generate active movement. Tendinopathy affects more than 10% of the population under 45 and compromises the tendon functions16,17. Tendon injury healing is slow and incomplete due to the low number of cells in tendon and the hypovascular and anaerobic environment18,19. Tendon stem/progenitor cells (TDSCs) are a cell population derived from tendon and considered to be a subgroup of mesenchymal stem cells that have abilities to improve tendon injury healing20,21. However, the number of TDSCs in tendon is low and retrieving TDSCs is invasive and exacerbates tendon injury. Morphology and proliferation ability loss in the culture system also hampers the efforts to obtain sufficient amounts of active TDSCs. To find more cell types supporting tendon regeneration will facilitate the development of regenerative medicine to treat tendon injury.
The activity of tenogenesis is tightly regulated by many signaling pathways, while TGFβ/SMAD2/3 is a crucial molecular axis22. TGFβ signaling is indispensable for tendon development23, and it could systematically promote the tenogenic differentiation of stem cells24,25. The downstream effectors SMAD2 and SMAD3 are able to activate the transcription of tendon-specific genes which further facilitates tendon development and tenogenic differentiation22,26,27. After tendon injury, TGFβ/SMAD2/3 promotes the proliferation, migration and differentiation of TDSCs28, increases tendon collagen synthesis29, and contributes to matrix anabolism for tendon remodeling30.
Here we found that CD56+ CD29+ human myogenic progenitor cells can be differentiated to tendon cells both in vitro and in vivo. Transplantation of human myogenic progenitor cells to injured tendon in mice improved the tendon regeneration, suggesting that human muscle myogenic progenitor cells are bipotential adult stem cells. Interestingly, the tendon differentiation potential in mouse muscle stem cells is minimal and the higher TGFβ signaling level in human myogenic progenitor cells may be the key for the distinct feature of human myogenic progenitor cells.
Results
Potential tenogenic differentiation potential of myogenic progenitor cells identified by single cell sequencing
To analyze the cell components of human skeletal muscle, single cell sequencing analysis was performed using skeletal muscle biopsy. A total of 15,102 cells were included for analysis. The cells were grouped to 8 cell populations (Fig. 1a and b). Consistent with the previous single cell sequencing results from human muscles31, Fibro/Adipogenic progenitors (FAPs), endothelial cells, muscle cells, and myogenic progenitor cells were among the identified cell types (Fig. 1a and b).
A population of tenocytes was identified in human muscle biopsies (Fig. 1a and b). Surprisingly, when we performed unsupervised trajectory analysis using the single cell sequencing data, two branches were identified by the analysis (Fig. 1c and d), while one branch linked muscle progenitor cells and tenocytes (Fig. 1c and d). RNA FISH of skeletal muscle section revealed that SCX, a key transcription factor determining tendon lineage, could be expressed in muscle stem/progenitor cells (Fig. 1e and f). These analysis results suggest that muscle stem cells could have tenocyte differentiation potential.
To confirm the single cell analysis results, we first isolated myogenic progenitor cells from human muscle biopsy using FACS as described previously6,12. CD45-CD31-CD56+ CD29+ cells were collected from the single cell suspension dissociated from muscle tissue. The collected cells were stained with anti-PAX7, anti-MYOD1 and anti-MYF5 antibody to confirm the purity of isolated myogenic progenitor cells (Fig. 1g and h). When induced to differentiate by 0.4% Ultroser G, human myogenic progenitor cells differentiate to myotubes robustly. Approximately 90% of nuclei were present in Myosin heavy chain (MyHC) positive myotubes (Fig. 1i and j). Consistently, the expression of PAX7, MYF5 and MYOD1 were enriched in the myogenic progenitor cells, their expression decreased after differentiation as shown by RT-qPCR assays (Fig. 1k). In contrast, the expression of genes marking differentiation such as Myogenin (MYOG), Myosin heavy chain 1 (MYH1), and Myosin heavy chain 3 (MYH3) were up-regulated in differentiated myotubes (Fig. 1l). Taken together, these results suggest that the myogenic progenitor cells isolated from human muscle biopsies have robust myogenic differentiation potential.
Human myogenic progenitor cells display tenogenic differentiation potential in vitro
The isolated primary human myogenic progenitor cells were induced to differentiate to tenocytes by 100ng/ml GDF5, 100ng/ml GDF7 and 0.2mM ascorbic acid for 12 days. After 12 days of tendon differentiation, the morphology of cells display dramatic differences from those undergoing myogenic differentiation (SFig. 1a). Furthermore, the expression of tendon markers such as TNC and SCX was significantly increased (Fig. 2a, b, and c). Moreover, some other tendon related genes, such as COL I, MKX, THBS4, and COMP, were also enriched after tendon differentiation induction (Fig. 2c). In contrast, the expression of these genes was not enriched upon myogenic induction (Fig. 2a, b, and c). The expression of genes marking myogenic differentiation such as MYOG and MyHC were only detected in a small portion of cells after tenogenic differentiation (SFig. 1b and c). Compared to the over 90% of differentiation efficiency upon myogenic differentiation induction, only about 20% of cells showed MYOG and MyHC expression after 12 days of tenogenic induction (SFig. 1b and c). The genes marking differentiated myotubes such as MYH1, MYH3, DESMIN, and MYL1 showed moderate elevation after tenogenic differentiation, while dramatic upregulation of these genes was observed after myogenic differentiation (SFig. 1d and e). These results combined suggest that human myogenic progenitor cells are capable of tendon differentiation in vitro.
To further confirm the results, we next performed clonal analysis. The freshly isolated primary human myogenic progenitor cells were seeded to 96 well plate with the concentration of 1 cell/well. The wells of single cell were allowed to proliferate for 10 days followed by tenogenic or myogenic induction. The plates with myogenic induction were differentiated for 4 days and immunofluorescence staining of MyHC was performed to determine muscle differentiation. The plates with tenogenic induction were differentiated for 12 days and immunofluorescence staining of SCX was performed to determine tenocyte differentiation (Fig. 2d). The number of wells showing positive MyHC staining was counted and the myogenic differentiation efficiency was calculated (Fig. 2e and g). MyHC+ myotubes was observed in over 95% of wells with alive cells (Fig. 2g). Similarly, the number of wells displaying positive SCX staining was counted and the tenogenic differentiation efficiency was calculated (Fig. 2f and g). Approximately 40% of myogenic progenitor cells displayed tenogenic differentiation ability (Fig. 2g), suggesting that human myogenic progenitor cells have tenogenic differentiation potential. Taken together, these results suggest that human myogenic progenitor cells have dual differentiation potentials towards muscle or tendon in vitro.
Tenocytes differentiated from human myogenic progenitor cells display similar expression profile to primary tenocytes
RNA sequencing was then performed to further determine the lineage of the tenocytes differentiated from myogenic progenitor cells. Myogenic progenitor cells were induced towards myogenic or tenogenic differentiation respectively. We also isolated primary human tenocytes as described previously32. These cells were subjected for RNA sequencing analysis. The differentiated myotubes and tenocytes displayed distinct expression patterns (Fig. 3a). The differentiated tendon cells share high similarity to fresh isolated tenocytes from human tendons (Fig. 3a), suggesting that human muscle stem/progenitor cells are capable of tendon differentiation. Consistently, two distinct sets of genes were up-regulated after myogenic induction and tenogenic induction, respectively (Fig. 3b and c). These results suggest that human myogenic progenitor cells are capable of dual direction differentiation
Further GO analysis also displayed the activation of two distinct sets of cell features. Upon the skeletal muscle differentiation induction, terms related to skeletal muscle functions such as skeletal muscle thin filament assembly, skeletal muscle contraction, muscle organ development, and sarcomere organization were enriched (Fig. 3d), suggesting the muscular identity of the differentiated cells. In contrast, terms related to tendon formation, tendon development, and tendon cell differentiation were enriched after tenogenic differentiation (Fig. 3e), suggesting that tendon identity is achieved in the differentiated cells. Together, these results suggest that human myogenic progenitor cells are capable of both myogenic and tenogenic differentiation in vitro.
Murine MuSCs display poor tenogenic differentiation ability
We next investigated whether the tenogenic differentiation ability is present in rodent muscle stem cells (MuSCs). Mouse MuSCs were isolated by positive marker of Vcam1 as described previously6 and induced for tenogenic differentiation. In sharp contrast to human myogenic progenitor cells, murine MuSCs failed to be induced to tendon cells upon the same induction condition as that for human myogenic progenitor cells, though the myogenic differentiation is as efficient as the human myogenic progenitor cells. After myogenic differentiation, 93.9% of nuclei were present in MyHC+ myotubes (Fig. 4a and b). In sharp contrast, no Scx+ cells were observed after 12 days of induction for tenogenic differentiation (Fig. 4a). Consistent with the immunofluorescence staining results, RT-qPCR results revealed that myogenic differentiation marker genes such as MyoG, Myh1, and Myh3 were up-regulated under both myogenic and tenogenic differentiation conditions (Fig. 4c), suggesting that murine MuSCs predominantly commit myogenic differentiation under induction. Different from human myogenic progenitor cells, the expression of genes indicating tendon cell fate such as Scx, Tnc, Col I, Mkx, and Thbs4 did not increase after tenogenic differentiation (Fig. 4d), suggesting the failure to induce tenogenic cell fate from murine MuSCs. To further rule out the bias caused by different FACs strategies between human and mouse myogenic progenitor cells, mouse muscle CD29+/CD56+ cells were isolated for tenogenic induction. However, very few mouse muscle CD29+/CD56+ cells expressed myogenic progenitor cell marker Pax7, MyoD1 and Vcam1, indicating the failure strategy to purify mouse myogenic progenitor cells by gating CD29+/CD56+ (SFig. 2a and 2b). Furthermore, RT-qPCR results of tendon related markers Scx, Tnc, Mkx, and Thbs4 revealed that there was also no tenogenic or myogenic differentiation potential of mouse muscle CD29+/CD56+ cells (SFig. 2c). Together, these results suggest that murine MuSCs display almost no tenogenic differentiation potential in vitro.
We next performed the lineage tracing experiments in mice to further examine the tenogenic potential of mouse MuSCs in vivo (Fig. 4e). Pax7CreERT2 mice were crossed to flox-Stop-flox-tdTomato mice. MuSCs and the descendants of MuSCs will be labeled by tdTomato (SFig. 2d). Tendon injury in mice was generated by mimicking the peroneus longus tendon removal surgery in human (SFig. 2e). In human, it has been reported that the tendon could be regenerated to some extent after the peroneus longus tendon removal surgery based on MRI imaging33. In this surgery, injury of the skeletal muscle adjacent to the removed tendon was inevitable. The accompanied skeletal muscle injury could activate MuSCs and make them available for tendon regeneration. To further activate MuSCs to guarantee that sufficient amount of activated MuSCs were available around the tendon injury site, we also injected cardiotoxin (CTX) at the muscle adjacent to the sites where the tendon was removed to induce more muscle injuries and further activate MuSCs. If MuSCs can participate tendon regeneration, tdTomato+ tendon cells would be observed after the reparation of tendon injury. As a control, muscle injury was induced by muscular injection of CTX at the muscles adjacent to the removed tendon.
Expectedly, large amount of tdTomato+ myofibers were observed after muscle injury (SFig. 2f-g), suggesting that the tracing system works well. Nevertheless, less than 0.2% tendon cells originated from mouse MuSCs were observed even four months after tendon removal (Fig. 4f-g). These results suggest that murine MuSCs have poor tendon differentiation abilities.
Transplantation of human myogenic progenitor cells facilitates tendon regeneration
We next went on to investigate whether the transplantation of human myogenic progenitor cells to mouse can improve tendon regeneration. An approximately 1.5mm long and 0.5mm width transverse incision was performed at 5mm from the calcaneus in Achilles tendon for NOD/SCID immunodeficient mice. Total 50,000 Pax7+ human myogenic progenitor cells packed in hydrogel were planted at the incision site (Fig. 5a). As a control, 50,000 murine MuSCs packed in hydrogel were transplanted in the SCID recipient mice undergoing the same tendon injury at the cleavage sites. Packing the cells with hydrogel concentrated the transplanted cells at the local injury sites. Two months after transplantation, the tendons carrying transplanted human myogenic progenitor cells or murine MuSCs were harvested, respectively (Fig. 5a).
In mice transplanted with human myogenic progenitor cells, continuous cryosections containing muscle and tendon tissues were generated. Immunofluorescence staining was performed to detect tendon and muscle markers with two cryosections adjacent to each other, respectively. The two sets of images obtained on continuous cryosections were superimposed on each other to pinpoint the position of tendons. Immunofluorescence staining of antibody specifically recognizing human TNC indicated the position of regenerated tendon-like tissue from human cells in the harvested tissues (Fig. 5b). The presence of human cells was also illustrated by the immunofluorescence staining with the antibody specifically recognizing human Lamin A/C. In the control PBS injection group, where the mixture of PBS and hydrogel instead human myogenic progenitor cells was injected to the incision sites, no human Lamin A/C was detected. These results confirmed the specificity of human TNC and Lamin A/C antibody. Immunofluorescence staining revealed that over 75% of the human cells showed TNC expression (Fig. 5b and d), suggesting that the majority of the transplanted human myogenic progenitor cells differentiate to tendon cells in vivo. The immunofluorescence staining of MyHC and human Lamin A/C was performed to detect the muscle cells originated from the transplanted human myogenic progenitor cells. Only about 12.8% of the human cells detected expressed MyHC (Fig. 5c and d). Moreover, the human cells were predominantly enriched at the tendon region (Fig. 5b). Only a few MyHC+ cells originated from the transplanted human myogenic cells scattered in the muscle region (Fig. 5c). To further confirm the tendon differentiation potential of the transplanted human myogenic progenitor cells, immunofluorescence staining of SCX and TNMD was also performed. The majority (80.0% and 74.6%) of transplanted human myogenic progenitor cells also express SCX and TNMD (SFig. 3a, b and c). Furthermore, Col I was predominantly expressed in the regenerated tendon-like tissue rather than Col III after human cells transplantation (Fig. 5e), indicating human myogenic progenitor cells contributes to structural repair of injured tendon and facilitates the healing process. Taken together, these results suggest that human myogenic progenitor cells are capable of tendon differentiation in vivo and contributing to tendon regeneration.
In sharp contrast to human myogenic progenitor cells, when 50,000 murine MuSCs constitutively expressing tdTomato were transplanted to pre-injured tendon in NOD/SCID mice under the same condition, less than 0.3% of tdTomato+ TNC+ cells were detected (Fig. 5f and g). However, the myogenic differentiation potential of human myogenic progenitor cells and mouse MuSCs was similar. Muscle injury was induced by muscular injection of CTX in tibialis anterior (TA) muscle in NOD/SCID mice. TA muscles were irradiated to kill the local MuSCs as described previously34. Transplantation of human myogenic progenitor cells and mouse MuSCs to the irradiated pre-injured recipient mice was performed, respectively. TA muscles were harvested after 28 days. Transplantation of both human myogenic progenitor cells and murine MuSC displayed robust engraftment efficiency (SFig. 3d-i). These results suggest that human myogenic progenitor cells and murine MuSCs have similar myogenic differentiation potential.
Combined, the above results suggest that human myogenic progenitor cells have dual differentiation potentials towards myogenesis or tenogenesis in vivo, while murine MuSCs predominantly commit myogenesis.
Transplantation of human myogenic progenitor cells improves locomotor function after tendon injury
We next checked whether the human myogenic progenitor cell transplantation improves tendon functions. First, transmission electron microscope analysis was used to evaluate microstructure of injured tendon two months after transplantation. Larger and denser collagen fibrils of the tendons were identified in transplanted group than control group with PBS injection, although the maturation level of collagen fibrils could still not reach uninjured tendon (Fig. 6a and b). We next examined whether transplantation of human myogenic progenitor cells could improve the biomechanical property of tendon. The max failure load and stiffness of the tendons from the transplantation group were significantly better than PBS injection control group (Fig. 6c). These results combined suggest that transplantation of human myogenic progenitor cells improves the collagen fibril maturation and biomechanical property of the injured tendon.
Whether the improved tendon regeneration could facilitate the whole organism locomotor function was next investigated. Since the Achilles tendon transmits the plantarflexion force from gastrocnemius muscle to calcaneus, the plantarflexion force of involved leg was also performed two months after tendon injury. Expectedly, transplantation of human myogenic progenitor cells for injured tendon also contributed to improving both twitch and tetanus plantarflexion force when compared with PBS injection control group, although the plantarflexion force could still not reach to level of uninjured leg (Fig. 6d). Consistent with the improved plantarflexion force, the endurance time and max fatigue speed of the mice transplanted with human myogenic progenitor cells was better in treadmill test when compared to no transplantation group (Fig. 6e). Since immunofluorescence staining with human Lamin A/C revealed that only a small number of human myogenic progenitor cells engrafted in muscle sporadically (Fig. 5c and d), these results suggest that the transplanted human myogenic progenitor cells can improve locomotor function by directly repairing injured tendon.
TGFβ signaling pathway contributes to tenogenic differentiation of human myogenic progenitor cells
Since the human myogenic progenitor cells and murine MuSCs shared the same strain of recipient mice and the same tendon injury while being transplanted, they had the similar microenvironment. Therefore, the distinct differentiation potentials are due to the cell intrinsic differences between species. We further compared the expression profiles of human myogenic progenitor cells and murine MuSCs. Interestingly, TGFβ signaling were identified in KEGG enrichment analysis of upregulated genes in human myogenic progenitor cells when compared with mouse muscle stem cells (Fig. 7a), indicating that TGFβ signaling could be the key node for maintaining the tendon differentiation potential. Furthermore, SMAD2 and SMAD3 were identified in upregulated gene set which was enriched in TGFβ signaling pathway (Fig. 7b). Since TGFβ/SMAD2/SMAD3 axis plays a crucial role in tendon development and tenogenic differentiation22,23,26, we next investigated the potential function of TGFβ signaling for tenogenic differentiation of human myogenic progenitor cells. Consistently, increased phosphorylated SMAD2/SMAD3 was confirmed after tenogenic induction of human myogenic progenitor cells (Fig. 7c). TGFβ signaling inhibitor SB-431542 was used to significantly impaired TGFβ/SMAD2/SMAD3 signaling during tenogenic induction (Fig. 7c). The immunofluorescent staining, western blot assay and RT-qPCR results showed SB-431542 significantly suppressed expression level of tendon related genes SCX, TNC, COL I, MKX, and THBS4 (Fig. 7d, e, f). On the contrary, myogenic differentiation ability of human myogenic progenitor cells was increased after treatment of SB-431542 during tenogenic induction (Fig. 7g and h). Taken together, these data indicated that TGFβ signaling pathway contributes to tenogenic differentiation of human myogenic progenitor cells.
Discussion
Here we show that human myogenic progenitor cells have dual differentiation potentials towards muscle and tendon. Transplantation of human myogenic progenitor cells contributes to injured tendon repair and thus improves locomotor function. Thus, the human myogenic progenitor cells could be served as a new source for tendon regeneration.
Tendon disorders widely occur in people of all ages22. It disrupts the stability and mobility of joint, which deeply affects their locomotor function and quality of life. However, the natural healing of injured tendon is very slow due to hypocellularity and hypovascularity of tendon. The biomechanical property and structural integrity could be hardly completely recovered even with surgical treatment35,36. It is still a great challenge in clinical work to treat tendon injury.
The relative inefficient outcome of routine therapy for tendon injury sparked the exploration of stem cell treatment. Seed cells with the ability to differentiate into tenocytes and secrete paracrine factors to repair tendon injury are preferred. Thus, tendon derived stem cells, embryonic stem cells, induced pluripotent stem cells and mensenchymal stem cells have been introduced as seed cells to treat tendon injury37. However, inadequate sources of tendon derived stem cells, ethical issue and risk of teratoma formation of embryonic stem cells or induced pluripotent stem cell, and heterogeneity of mensenchymal stem cells limit the development of these seed cells for tendon injury treatment. As for muscle progenitor cells, it is high in proliferation and abundant in sources, and donor site morbidity after muscle harvest was low. Thus, muscle stem cell might be a promising candidate as seed cells for tendon repair.
Here we also find that the differentiation potential of muscle stem cells is species dependent. Human muscle myogenic progenitor cells are bipotential adult stem cells. In contrast, murine muscle stem cells barely have tendon differentiation potential. The species difference might be due to the higher TGFβ/SMAD2/SMAD3 signaling level in human myogenic progenitor cells. TGFβ/SMAD2/SMAD3 axis plays a crucial role in tendon development and tenogenic differentiation22,23,26. SMAD2/3-dependent TGFβ signaling also acts as a crucial molecular brake for myogenesis. It could suppress myogenic regulatory factors Myod1 and Myogenin38,39, as well as inhibit myotube fusion and muscle regeneration40,41. The elevated TGFβ/SMAD2/SMAD3 signaling may help inhibit the myogenic differentiation ability and stimulate the tenogenic differentiation potential of human myogenic progenitor cells under specific microenvironment.
Studies using mouse models contributed to the majority of our knowledge about muscle stem cells and laid the foundation for our understanding of mammalian muscle stem cells. However, human is dramatically different from mouse in many aspects such as size, life span, and manners of motions. It is more demanding for humans to maintain the homeostasis of the locomotion system and the whole organism locomotion ability in much longer life span and bigger body size. Though our knowledge about human muscle regeneration is limited, the current studies have revealed multiple differentiation potentials of Pax7+ human progenitor cells in skeletal muscles. CD56+ CD34+ progenitor cells in human skeletal muscle have been reported to have myogenic, osteogenic, and adipogenic activity14,15. The CD56+ CD34-progenitor cells in human skeletal muscle have been shown to be free of adipogenic potential11. Our results suggest that the progenitor cells in skeletal muscles also have tenogenic differentiation ability besides their myogenic differentiation ability. It seems that there are multiple subpopulations of myogenic progenitor cells in skeletal muscle. They are all capable of muscle regeneration, while with potentials to regenerate other components of the motion system such as bone, tendon, and adipocytes. This could be an economic method to maintain the functions of the motion system for the longer life span and more complicated motion manner in human beings.
Methods
Animals
Animal care and use were in accordance with the guidelines of the animal facility hosted by Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, and the operations were approved by the ethical committee of Shanghai Institute of Biochemistry and Cell Biology. All mice were maintained in specific pathogen-free (SPF) animal facility in individually ventilated cages (IVC) with controlled temperature (22±1°C) and light (12h light/dark cycle). NOD/SCID mice were purchased from Animal Model Research Center of Nanjing University. Pax7CreERT2 and Rosa26-Flox-Stop-Flox-tdTomato mice were purchased from Jackson Laboratory. All experiments were conducted on 3-month-old adult male mice.
Human samples
Peroneal longus muscle and remanent tendon were obtained from the wastes of patients who underwent full-thickness peroneal longus tendon autograft for knee ligament reconstructive procedures. The study was approved by the ethical committee of Xinhua Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine (Approval No. XHEC-D-2019-043) and written informed consents were obtained from all donors.
Isolation of human muscle stem/progenitor cells
Human skeletal muscle from peroneal longus were dissected and digested as described previously6. Briefly, muscle tissues were cut into small pieces and digested by collagenase II (Worthington biochemical, 700-800U/ml, cat#LS004177) for 60min followed by 30min of digestion with the mixtures of collagenase II and dispase (Life Technologies,11U/ml, cat#17105-041). Digested cells were passed 10 times through a 20-gauge needle. Cell suspension was filtered through a 40µm cell strainer (BD Falcon, cat#352340). Erythrocytes were removed by red blood cell lysis buffer (Thermo Fisher Scientific, cat#00-433-57). The single cell suspension obtained from human muscle was stained with a cocktail containing PE-Cy5 anti-human CD45 (BD Pharmingen, cat#555484), Percp-Cy5.5 anti-human CD31 (BioLegend, cat#303132), AF-488 anti-human CD29 (BioLegend, cat#303016) and PE anti-human CD56 (BioLegend, cat#304606) for 45 min at 4°C. CD45-CD31-CD29+ CD56+ human muscle stem/progenitor cells were sorted by BD Influx sorter (BD Biosciences).
Isolation of mouse muscle stem cells
For mouse muscle stem cells isolation, dissected TA muscles were first digested with 10ml muscle digestion buffer (DMEM containing 1% penicillin/streptomycin, 0.125mg/ml Dispase II (Roche, 04942078001), and 10mg/ml Collagenase D (Roche, 11088866001)) for 90 minutes at 37°C. The digestion was stopped by adding 2ml of FBS. The digested cells were filtered through 70μm strainers. Red blood cells were lysed by 7ml RBC lysis buffer (0.802% NH4Cl, 0.084% NaHCO3, 0.037% EDTA in ddH2O, pH7.2-7.4) for 30s, then filter through 40μm strainers. After staining with antibody cocktails (AF700-anti-mouse Sca-1, PerCP/Cy5.5-anti-mouse CD11b, PerCP/Cy5.5-anti-mouse CD31, PerCP/Cy5.5-anti-mouse CD45, FITC anti-mouse CD34, APC-anti-mouse Integrin α7+), the mononuclear cells were subjected for FACS analysis using Influx (BD Biosciences). The population of PI-CD45-CD11b-CD31-Sca1-CD34+ Integrin α7+ cells was collected.
Primary human tenocytes isolation
Tendon tissues were obtained from the discarded materials of tendon autograft surgery. They were washed with PBS. Epi- and peri-tendon sheath were completely removed. Tenocytes were isolated as described previously20. Briefly, the tendons were minced to 1mm3 pieces and digested with 3 mg/ml collagenase I (Worthington biochemical, cat# LS004194) in DMEM (Gibco, cat#11965118) at 37°C for 3hrs with gentle agitation. The digested tissue was filtered through a 40µm cell strainer (BD Falcon, cat#352340) and the isolated cells were plated for subsequent analysis.
Cell culture and differentiation
Primary human muscle stem/progenitor cells were plated in F10 basal medium (Gibco, cat#11550043) containing 20% FBS (Gibco, cat#10-013-CV), 2.5ng/ml bFGF (R&D, cat#233-FB-025) and 1% Penicillin-Streptomycin (Gibco, cat#15140-122) on collagen-coated dishes. Mouse muscle stem cells were plated in F10 basal medium (Gibco, cat#11550043) containing 20% FBS (Gibco, cat#10-013-CV), 2.5ng/ml bFGF (R&D, cat#233-FB-025) and IL-1α, IL-13, IFN-γ and TNF-α as described previously34. DMEM (Gibco, cat#11965118) containing 0.4% Ultroser G (Pall Corporation, cat#15950-17) and 1% Penicillin-Streptomycin were used to differentiate human muscle stem/progenitor cells 5. DMEM containing 2% horse serum (HyClone, cat#HYCLSH30074.03HI) and 1% Penicillin-Streptomycin were used to differentiate mouse muscle stem cells as described previously42,43. DMEM containing 10% FBS, 100ng/ml GDF5 (R&D, cat#8340-G5-050), GDF7 (R&D, cat#8386-G7-050), 0.2mM ascorbic acid (Sigma-Aldrich, cat#A4403) and 1% Penicillin-Streptomycin were used to induce tendon differentiation 44–46. Primary tenocytes were cultured in DMEM containing 20% FBS, 2.5ng/ml bFGF (R&D, cat#233-FB-025) and 1% Penicillin-Streptomycin.
Single-cell RNA sequencing and trajectory analysis
The single cell suspension obtained from human muscle was firstly prepared. PI (Sangon Biotech, cat#E607328) and Hoechst (Sangon Biotech, cat#A601112) were used to sort live cells by FACS. Then the sored cells were washed twice with PBS containing 0.04% BSA, followed by library preparasion with Chromium Single Cell 3’ Reagent Kits (10X genomics, cat# 1000121-1000157). The sequencing was performed on Illumina Novaseq 6000 platform (Illumina).
Single cell RNA-seq data were analyzed by Seurat R (Version 3.2.0) package. Cells with less than 200 genes, more than 6,000 genes detected, and more than 10% mitochondrial genes were excluded. A total of 15,102 cells were included for subsequent analysis. Sequencing reads for each gene were normalized to total UMIs in each cell to obtain normalized UMI values by “NormalizeData” function. The “ScaleData” function was used to scale and center expression levels in the data set for dimensional reduction. Total cell clustering was performed by “FindClusters” function at a resolution of 0.1 and dimensionality reduction was performed with “RunUMAP” function. Trajectory analysis was done by R package Monocle247.
Immunofluorescence staining
Cryosections were fixed in 4% formaldehyde for 15min, permeabilized in 0.5% Triton X-100 for 15min, and stained with anti-Pax7 (Developmental Studies Hybridoma Bank), anti-Laminin (Abcam, cat#ab11575), anti-Lamin A/C (Abcam, cat#ab108595; cat#ab190380), anti-TNC (Abcam, cat#ab108930; cat#ab3970), anti-Scx (Abcam, cat#ab58655), anti-Tnmd (Abcam, cat#ab203676), anti-Col I (Abcam, cat#ab260043), anti-Col III (ABclonal, cat#A0817) or anti-MyHC (Millipore, cat#05-716) at 4°C overnight, and incubated with Alexa 488-, Alexa 594 or Alexa 647-labeled anti-mouse or anti-rabbit secondary antibodies (Invitrogen, 1:1000) at room temperature for one hour. The nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, cat#H-1200). All images were acquired by Leica SP8 confocal microscope (Leica).
RNA scope in situ hybridization
Fresh cryosections of human muscle were obtained. Then the target gene-specific RNAscope probes (RNAscope Probe-Hs-SCX, cat#593041; RNAscope Probe-Hs-PAX7-C2, cat#546691) were used to investigate the cellular distributions of Pax7 and SCX. All staining steps strictly followed the manufacturer’s protocols by Advanced Cell Diagnostics. The nuclei were then stained with 4,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, cat#H-1200), and sections were coverslipped with antifade mounting media (Vector Laboratories, cat#H-100). All images were acquired by Leica SP8 confocal microscope or Olympus BX53 fluorescence microscope (Leica).
Gene expression analysis
Total RNA was isolated using TRIzol Reagent (Invitrogen, cat#15596-018) according to the manufacturer’s instruction. GAPDH served as internal control. The primers for RT-qPCR are listed as below:
Cloning assay
Primary human muscle stem/progenitor cells were first sorted in 96 well plates with density of single cell per well. Tenogenic induction or myogenic induction was performed after proliferation for each well. After induction, the immunofluorescence staining of SCX or MyHC was performed in each well. The differentiation efficiency was determined by calculating the ratio of total wells with positive fluorescence signal to total wells with alive cells.
RNA-sequencing
Total RNA was isolated using TRIzol Reagent (Invitrogen, cat#15596-018) according to the manufacturer’s instruction. mRNA was enriched with magnetic oligo (dT) beads (New England Biolabs, cat#S1419S). The cDNA library was constructed with mean inserts of 200bp with non-stranded library preparation using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, cat#E7530L). Sequencing was performed by a paired-end 125 cycles rapid run on the Illumina HiSeq2500. Sequencing data were filtered by SolexaAQ (Q > 20 and length ≥ 25 bp)48.The adapter sequences and low quality segments (Phred Quality Score<20) were trimmed using Cutadapt. Paired-end clean reads were then mapped to the reference genome GRCh38.98 using HISAT2. Htseq-count was used to quantify the gene expression value49. Read count of each gene was normalized using FPKM (Fragments Per Kilo bases per Million fragments). Differential expression (DE) analysis was performed using DESeq and significant DE genes were defined as those with absolute log2FoldChange>1 and p< 0.05. Heatmap and volcano plot of DE genes was generated by R package Pheatmap (https://cran.r-project.org/web/packages/pheatmap/index.html) and EnhancedVolcano (https://bioconductor.org/packages/release/bioc/vignettes/EnhancedVolcano/inst/doc/Enh ancedVolcano.html), respectively. Gene enrichment analysis was conducted by R package topGO with p < 0.05 as the cut-off. The differential expression analysis for RNA-seq data between human and mouse myogenic progenitor cells was performed according to previous literature50.
Cell transplantation in TA muscle
A single dose of 18 Grey irradiation was administered to the hind legs of the recipient NOD/SCID mice. TA muscle was injured by injecting 15μl of 10μM CTX (Sigma), and 50,000 human myogenic progenitor cells or 50,000 murine MuSCs suspended in 10μl PBS were injected intramuscularly to the injury site as described 5.
Tendon injury for lineage tracing
Pax7CreERT2:Rosa26tdTomato mice were obtained and MuSC was labeled with tdTomato as aforementioned. We used dedicated surgical instruments to imitate a mouse model similar to previously published human surgery33. Firstly, distal gastrocnemius tendon was woven with a 6-0 polypropylene non-absorbable suture (PROLENE, cat#EH7242H) and released by microsurgical scissor. After that, a dedicated mini tendon stripper was introduced over the free end of distal medial gastrocnemius tendon. The medial gastrocnemius tendon could be totally removed after advancement of tendon stripper. To further activate MuSCs to guarantee that sufficient amount of activated MuSCs were available around the tendon injury site, cardiotoxin (CTX) was injected at the muscle adjacent to the sites where the tendon was removed to induce more muscle injuries and further activate MuSCs. Then the subcutaneous tissue and skin were sutured.
Tendon injury and cell transplantation
An approximately 1.5mm long and 0.5mm width transverse incision was performed at 5mm from the calcaneus in Achilles tendon for recipient NOD/SCID mice. 50,000 human muscle stem/progenitor or mouse muscle stem cells resuspended in 20μl hydrogel were injected to the injury site with 28-gauge needles. PBS mixed with 20μl hydrogel were injected as control.
Biomechanical analysis
Two months after tendon injury, the tendons were harvested to biomechanical analysis using a universal tester (Instron 3345 load system, USA). The grippers were gradually moved apart with the speed of 5 mm/min until the tested tendon was completely ruptured. Then the max load was obtained and documented. The slope of the stress-strain curve was defined as stiffness. All these data were automatically presented in Instron 3345 load system.
Transmission electron microscope (TEM) examination
After washing the selected target tendon tissues with PBS, tissue fixation was performed for more than 2 hours. Post-fixation was conducted by 1% OsO4 (Ted Pella Inc) in 0.1M PB for 1-2 hours. Then dehydration and drying were performed and target tendon samples were subsequently attached to metallic stubs for conductive metal coating. Images were obtained by TEM (HITACHI, SU8100) and at least 1000 collagen fibrils were evaluated for each sample. Density of fibrils was evaluated by the percentage of collagen fibril containing area.
Treadmill test
Mouse treadmill test was performed in Exer-3/6 treadmill apparatus with electrical stimulus. Treadmill exercise began with a 5 min warm-up, then each mouse was evaluated with specific protocol. For endurance test, mice ran on the treadmill at the constant speed of 22m/min until exhaustion. For exhaustion test, the treadmill speed was increased (2m/min each 3 min) at the beginning speed at 18m/min until exhaustion. Exhaustion was defined as inability to run on the treadmill for longer than10 seconds.
In vivo muscle force analysis
The 1300A 3-in-1 whole animal system (Aurora Scientific) was used for in vivo muscle force analysis. Mice were first anesthetized and kept warm by heat lamp. The foot was placed on a footplate and kept perpendicular to the tibia. The peroneal branch of the sciatic nerve was stimulated to evaluate the plantarflexion strength. Five repetitive tests were performed for each limb and DMA software (Aurora scientific) was used for results analysis.
Statistical analysis
At least three biological replicates and technical repeats were performed in each experimental group. All experiments were analyzed and evaluated by investigators in a blinded manner. Error bars indicated standard deviation. Two-tailed unpaired Student’s t-tests were used when variances were similar (tested with F-test) for comparison of two independent groups. One-way ANOVA tests followed by Dunnett’s post-test or Tukey’s post-test were used for multiple comparisons. Shapiro-Wilk tests were performed to determine data normality. Statistical analysis was performed in GraphPad Prism 7 (GraphPad Software, San Diego, USA) or SPSS version 19.0 for Windows (SPSS Inc., Chicago, IL, USA). It was considered significant with P value less than 0.05. Data were presented as mean ± standard unless stated.
Accession numbers
The complete sequencing data have been uploaded on to Sequence Read Archive database (PRJNA1012476 and PRJNA1012828).
Acknowledgements
We thank Dr. Dangsheng Li for helpful discussions, the National Protein Science Center (Shanghai) for helps on FACS sorting, the cell biology facility of SIBCB for helps on imaging and FACS analysis. This work was supported by the Strategic Priority Research Program of the Chinese Academy of Science (XDA16020400), National Science Foundation of China (32170804, 81871096, 82372384, 82302657).
Supplemental Figure legend
Data availability
The datasets used in this study can be obtained from the corresponding author upon reasonable request.
Conflict of interest disclosure
The authors declared no conflict of interest.
Funding
This work was supported by the Strategic Priority Research Program of the Chinese Academy of Science (XDA16020400), National Science Foundation of China (32170804, 81871096, 82372384, 82302657).
Contributors
XS, XL, HL, HZ, and XF designed the study, collected the experimental data and wrote the original draft. SL and SZ conducted the image analysis, statistical analysis. SZ designed the study, reviewed and edited the manuscript. JW and PH administrated the project and edited the manuscript. Xiexiang Shao, Xingzuan Lin and Hao Zhou contributed equally to this work.
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