Abstract
Lipopolysaccharides (LPS) confer resistance against harsh conditions, including antibiotics, in Gram-negative bacteria. The lipopolysaccharide transport (Lpt) complex, consisting of seven proteins (A-G), exports LPS across the cellular envelope. LptB2FG forms an ATP-binding cassette transporter that transfers LPS to LptC. How LptB2FG couples ATP binding and hydrolysis with LPS transport to LptC remains unclear. We observed the conformational heterogeneity of LptB2FG and LptB2FGC in micelles and/or proteoliposomes using pulsed dipolar electron spin resonance spectroscopy. Additionally, we monitored LPS binding and release using laser-induced liquid bead ion desorption mass spectrometry. The β-jellyroll domain of LptF stably interacts with the LptG and LptC β-jellyrolls in both the apo and vanadate-trapped states. ATP binding at the cytoplasmic side is allosterically coupled to the selective opening of the periplasmic LptF β-jellyroll domain. In LptB2FG, ATP binding closes the nucleotide binding domains, causing a collapse of the first lateral gate as observed in structures. However, the second lateral gate, which forms the putative en try site for LPS, exhibits a heterogeneous conformation. LptC binding limits the flexibility of this gate to two conformations, likely representing the helix of LptC as either released from or inserted into the transmembrane domains. Our results reveal the regulation of the LPS entry gate through the dynamic behavior of the LptC transmembrane helix, while its β-jellyroll domain is anchored in the periplasm. This, combined with long-range ATP-dependent allosteric gating of the LptF β-jellyroll domain, may ensure efficient and unidirectional transport of LPS across the periplasm.
Introduction
Antibiotic resistance poses a critical threat to global health, necessitating a comprehensive understanding of bacterial defence mechanisms. The outer membrane (OM), a crucial component of Gram-negative bacteria, serves as an initial line of defence against antibiotics1. Lipopolysaccharides (LPS) constitute a major component of the OM. LPS is a glycolipid consisting of lipid A, core oligosaccharides and an O-antigen2. The LPS transport (Lpt) system plays a central role in the biogenesis of OM, contributing significantly to its impermeability and resilience against environmental stress and host defenses3-8. The pathways for LPS synthesis, transport and regulation offers potential targets for novel antibiotics9-13. Comprising of seven essential components LptA-G (Figure 1A), the system orchestrates unidirectional translocation of LPS from the inner membrane (IM) to the OM6,14. The LptB2FG complex forms an ATP-binding cassette (ABC) transporter in the IM4,15-17. The LptB subunits constitute the nucleotide-binding domains (NBD)18 and interact in a head-to-tail manner to create two nucleotide binding sites (NBSs, for ATP or ADP). The F and G subunits together form the transmembrane domains (TMDs) to create the LPS binding pocket19-21. The pseudo two-fold symmetry of the TMDs creates two lateral gates on either side of the TMDs. LptC connects LptF-LptG20,22,23 with LptA24 to transfer LPS through the trans-periplasmic bridge towards LptDE25-28.
Previously, LptB2FG apo structures revealed open NBDs with bound LPS inside the TMDs (Figure 1B-C)20,22,29. Upon vanadate trapping, the NBDs dimerize causing a collapse of the LPS binding pocket, which has been suggested as a post-translocation state (Figure 1D-E)20,23. Presence of LptC enlarged the cavity leading to a weaker interaction with LPS (Figure S1A-B)20,22,23. In this conformation, the transmembrane (TM) helix of LptC weakly interacts with LptG, but forms extensive hydrophobic interactions with TM5 of LptF. The periplasmic β-jellyroll domains of LptF and LptG are very flexible leading to a reduced resolution. LptB2FGC structures in the AMP-PNP or vanadate-trapped state did not reveal the transmembrane helix of LptC (TM-LptC), thereby revealing a nearly identical conformation of NBDs and TMDs as with the LptB2FG structures20,23. Also, the β-jellyroll domains were barely resolved (Figure S1C-D).
It was proposed that upon ATP binding, TM-LptC moves away from the LptG-TM1 – LptF-TM5 interface. This would lead to a collapse of the binding pocket and expulsion of LPS to the β-jellyroll domain of LptF. However, several details of this mechanism such as when and how TM-LptC dissociates and in what way that is transmitted to the interaction and dynamics of the β-jellyroll domains remain poorly understood. Likely, many intermediate states might exist between ATP binding and LPS translocation and the relevance of the available structures in a native-like lipid environment is unknown. Altogether, an integrated characterization of the intermediate structures and underlying dynamics is required to elucidate the functional mechanism. Here we characterized the conformational heterogeneity of LptB2FG and LptB2FGC using extensive pulsed dipolar electron spin resonance (ESR) spectroscopy experiments, which permits observations in micelles, lipid bilayers or even in the native environments for certain cases 30-41. These observations were further correlated with results obtained using laser-induced liquid bead ion desorption mass spectrometry (LILBID-MS)42,43. Our results provide novel insights into the allosteric regulation of the structure and dynamics of the TMDs and the β-jellyroll domains during ATP binding and hydrolysis, which altogether drive LPS translocation.
Results
Conformational heterogeneity of LptB2FG in micelles and liposomes
Initially, we characterized the dynamics of LptB2FG in both DDM micelles and proteoliposomes (PLS) using pulsed electron-electron double resonance (PELDOR or DEER) spectroscopy44,45. This technique has been used for investigating several Type I, type II, type III and type IV ABC transporters 31,38,46-52. LptB2FG belongs to the type VI class of ABC transporter. For this purpose, we engineered spin pairs along the NBDs, TMDs and the β-jellyroll domains (Figure 2-6, Figure S2). Following reaction with a cysteine, the (1-Oxyl-2,2,5,5-tetramethyl-3-pyrroline-3-methyl)methanethiosulfonate (MTSL)) label creates the side chain denoted as R153. The functionality of all the spin labelled variants after size-exclusion chromatography was confirmed using ATP-ase activity assay (Figure S3-S4). All the cysteine variants could be labelled with high efficiency (Figure S5 and Table S1). We investigated LptB2FG under several conditions including the apo-state or after incubating with ATP-EDTA (EDTA to chelate divalent metal ions and to inhibit any residual ATPase activity), ATP-Mg2+-VO43- (vanadate-trapped transition state) or ADP-Mg2+ (post-hydrolysis state).
In agreement with the apo structure, the NBDs displayed a mean interspin distance centred around 5 nm in micelles when observed at position B_M134R1 (Figure 2A-D). This distance decreased below 4 nm in the vanadate-trapped state. With ATP(EDTA) a mixed population was observed with the major peak corresponding to the closed conformation. Thus, Mg2+ ions are not necessary for the closure of the NBDs. With ADP-Mg2+ the NBDs do not close, though the overall flexibility is somewhat increased (as gauged from the width of the distribution). We performed additional experiments after reconstitution into PLS (Figure 2F-G). Overall, the data in the apo-state revealed a similar mean distance, yet with a larger width suggesting an increased dynamics. As observed in micelles, vanadate-trapping closed the NBDs in PLS as well. Overall, the ATP-induced closure as observed in micelles (and the structures) is maintained in the native-like lipid bilayers for the NBDs.
Conformational heterogeneity of the lateral gates of LptB2FG in micelles and PLS
We introduced two spin pairs to independently monitor the lateral gates of the TMDs. The lateral gate formed by LptF-TM1 – LptG-TM5 and LptG-TM1 – LptF-TM5 were monitored using F-A45R1_G-I325R1 and F-L325R1_G-A52R1 pairs, respectively. For the apo-sate in micelles, the distances for the lateral gate-1 are similar to the corresponding simulation on the LPS-bound structure (Figure 3A). Vanadate-trapping resulted in an increase of the distances in agreement with the simulation. Though the central cavity collapses, this increased distance results from a coordinated movement of LptF-TM1 towards the cavity accompanied with an outward shift of LptG-TM5 (Figure 3E and Figure S1-S2). Addition of ATP(EDTA) resulted in an equilibrium between the two conformations. Altogether, the PELDOR data validate a LPS binding competent conformation in the apo- and ADP-Mg2+ states accompanied with a collapse of the gate in the ATP and vanadate-trapped post-hydrolytic state. Interestingly, for the apo state in PLS, this gate exhibited a broader distribution spanning the range corresponding to both structures (Figure 3F-G). In the vanadate-trapped state the overall distribution got narrowed (the minor peak at a longer distance is not interpreted due to a larger uncertainty). However, the overall spread of the distribution remained similar, suggesting a more flexible conformation of this gate in PLS, which is minimally affected by ATP binding.
The putative lateral gate for LPS entry exhibits a large conformational heterogeneity
Interestingly, the lateral gate-2 between LptG-TM1 – LptF-TM5 revealed a more dynamic behaviour in micelles (Figure 4A-D). In the apo state, it has a broad conformational distribution spanning the LPS bound (open) and vanadate-trapped (closed) structures (corresponding simulations are shown in orange lines). In the vanadate-trapped and ATP samples, the major population is centred at 2 nm (which corresponds to the simulation on the vanadate-trapped structure). The decreased interspin distances arise from a collapse of the cavity upon vanadate-trapping (Figure 4E and Figure S1-S2). Unlike the lateral gate-1 (and the NBDs), ADP-Mg2+ also induced a similar shift in the distance distribution. Why ADP-Mg2+ induce a change for this gate (alone) calls for further investigation. An asymmetric interaction of the cytoplasmic loop2 (linking TM4 and TM5 in both LptF and LptG), which was shown to interact with nucleotides20 could have a role here. In PLS as well, the apo state revealed a broad distribution validating the heterogeneity in the membrane environment (Figure 4F-G). Vanadate-trapping somewhat narrowed the distribution with (only) a fraction of the distances overlaying with the simulation for the corresponding structure (with a more pronounced heterogeneity as compared with the micellar sample, Figure 4B). Position L325 investigated here is located on short loop between TM5 and TM6 in LptF. This loop is resolved with clear density and has a similar orientation in the apo and vanadate-trapped structures with minimum deviation (Figure 4E, S6). Also, the experimental data in the vanadate trapped state is in agreement with the shift of the helices as observed from the structure (Figure 4B, E and Figure S1-S2). Therefore, the internal flexibility of this short loop might have the least contribution to the broad distribution we experimentally observed. Comparing this heterogeneity from the PELDOR data (Figure 4) with the corresponding simulations (in orange, Figure 4A-B), it appears that the structures captured two of the states from the broad conformational space. This gate is suggested to be the entry point for LPS22 and the enhanced dynamics we observed might be required for efficient interaction with LPS and LptC (please see later sections). Supporting these observations, LptG-TM1 and LptF-TM5 helices interact rather weak in the structures, in particular in the LPS-bound state (Figure 1C, E and Figure S1-S2).
Conformational dynamics of the β-jellyroll domains
We further investigated the interaction between the β-jellyroll domains in LptB2FG. Distances between positions F-S186R1 and G-V209R1 located on these domains revealed a major peak in agreement with simulation on the LPS-bound structure (Figure 5A-B) and a previous study56 employing isolated β-jellyroll domains. The overall spread of the distribution remained rather similar in the vanadate-trapped state revealing a stable interaction. We also probed the internal flexibility of LptF β-jellyroll using F_S156R1– I234R1 and F_S186R1 – I234R1 pairs, which are located on the putative entry gate loops for LPS22 (Figure 5C, E-F). The LptF β-jellyroll in the apo-state showed a broad distribution, but with a major population staying in the closed conformation as observed in the structure (Figure 5E, F). Interestingly, in the vanadate-trapped state, the overall distribution shifted towards longer distances revealing an opening of the LPS entry gate. Altogether, these observations reveal a long-range allosteric coupling between nucleotide binding at the NBDs in the cytoplasm and opening of the LPS entry gate of LptF β-jellyroll in the periplasm. Such a tight coupling might prime this domain to receive LPS once it is released from the cavity inside the TMDs. A strikingly different response was observed for the β-jellyroll domain of LptG. The equivalent spin pairs G_V209R1 – G_L234R1 and G_L152R1 – V209R1 revealed a considerably enhanced flexibility independent of ATP (Figure 5D, G-H) and revealing no such allosteric coupling with the NBDs.
Conformational heterogeneity of LptB2FGC
Next, we investigated how LptC modulates dynamics of the lateral gates and the β-jellyroll domains in LptB2FG. Measurements between the β-jellyroll domains of LptF and LptC (F_L219R1 – C_T104R1) revealed a narrow distance distribution and hence a stable interaction between them in both apo and vanadate-trapped states (Figure 6A-B). At the lateral gate-1 (F_A45R1-G_I335R1, Figure 6E and F-G), the distance in the apo state is similar to the simulation on the structure. In the vanadate trapped state, the spread of the distance distribution is increased, spanning the range of the simulations corresponding to both apo and vanadate-trapped structures (in the latter structure, TM-LptC is absent at the gate leading to a collapse of the cavity and an identical conformation with LptB2FG, though the distances increase due to an outward movement of LptG-TM5, see Figure 3B,E and Figures S1-S2). Distances corresponding to the apo state are present possibly due to an incomplete vanadate-trapping (or an equilibrium between the two conformations, which is rather unlikely) for this sample. Overall, the results support TM-LptC dissociation in the vanadate-trapped state as observed in the structure.
A distinct response is observed for the lateral gate-2 (F_L325R1 – G_A52R1, Figure 6A and C-D). LptC binding reduced the overall flexibility of this gate into two defined distance peaks as compared to LptB2FG alone (Figure 4A vs.6C). LptC binding is shown to increase the separation between these helices (Figure S1A). The major distance peak (centred around 2 nm) is shorter than the simulation on the LptB2FG apo structure (in magenta, Figure 6C, top panel), which therefore likely represents a conformation in which TM-LptC is released. The second peak corresponds to a larger separation between these helices, possibly representing the structure having the TM-LptC inserted at the gate (as position L325 is not resolved in the E. coli structure, corresponding simulation is not presented). The first conformation appears to be more favoured under the experimental conditions. In the vanadate-trapped state, the second peak disappeared and the overall distribution centred towards the TM-LptC released (LptB2FG) conformation (Figure 6D). This is in line with the lack of density for TM-LptC in the vanadate-trapped LptB2FGC structure. The experimental distribution is longer than the corresponding simulation (orange line in Figure 6D), suggesting a somewhat farther separation between these helices.
We further probed LPS release in LptB2FG using LILBID-MS (Figure 6H-J). In the apo and vanadate-trapped states, the majority of the observed complex peaks represent LPS bound to LptB2FG, while some LPS free LptB2FG can as well be seen (indicated in grey and red shades, respectively for both 1- and 2-charge states). Presence of LPS-bound LptB2FG in the vanadate-trapped state is not unexpected as the PELDOR data revealed a heterogenous conformation for the LPS entry gate other than the collapsed conformation (Figure 4B and H). However, under the hydrolysing conditions, LPS is completely released from LptB2FG.
Discussion
LPS transport by LptB2FG remained elusive for many reasonsLptB2FG and LptB2FGC structures revealed a similar structure in the vanadate-trapped state leaving no trace for the TM-LptC and the β-jellyroll domains (Figure S1D). Available structures possess either AMP-PNP or ADP-VO43- as nucleotides (both in a closed conformation of the TMDs and NBDs), thereby limiting details on how ATP binding and hydrolysis are coupled to the conformational changes and LPS transport.
For the NBDs and the two lateral gates of LptB2FG, PELDOR data validated the conformational changes in micelles as observed from the structures. Upon ATP binding and/or vanadate-trapping, the NBDs and the two lateral gates moved in a coordinated manner to collapse the LPS binding pocket (Figures 2-4). However, the lateral gate-2 forming the LPS entry site (TM1G-TM5F monitored by F_L325 – G_A52, Figure 4, S1 and S2) exhibits a very flexible conformation (even with nucleotides), which might facilitate interaction with LPS and or LptC. In agreement, LptC binding restricted the overall flexibility of this gate into two distinct conformations corresponding to a collapsed and an open conformation in which TM-LptC is likely inserted into the TMDs (Figure 6C, top panel). The minor peak at longer distances might corresponds to a conformation in which TM-LptC and LPS can enter the TMDs while the β-jellyroll domains of LptC and LptF interact. (Figure 6B). The minimal interaction between the corresponding helices (Figure S1-S2) in the LptB2FG structures suggest that the flexibility is inbuilt and might have an important role for the function. The PLS environment modulates the observed conformation in LptB2FG. The lateral gate-1 has a broader distribution in PLS, which is minimally affected by vanadate-trapping (Figure 3F-G). Also, the heterogeneity for the lateral gate-2 is more pronounced in PLS. Yet, the observations are qualitatively similar to the micellar sample (also see Figure S7 for the continuous wave (cw) ESR spectra) and further experiments are required to clarify how the membrane would influence LptB2FGC conformation.
In LptB2FG, the two β-jellyroll domains stably interact in the apo and vanadate-trapped conditions (Figure 5). Interestingly, binding of nucleotides is allosterically coupled to a selective opening of LptF β-jellyroll with little effect on the LptG β-jellyroll (Figure 5E-H). This opening was shown to be essential for cell growth and it was suggested that movement of LPS, but not ATP binding or hydrolysis pushes the gate open22. However, our results confirm that the gate opening is allosterically regulated through ATP binding and/or hydrolysis. This β-jellyroll start from TM3 and ends on TM4. TM3 is connected to the coupling helix and TM4 is connected to the cytoplasmic loop-2, which harbours the conserved R292 directly interacting with the nucleotide20. It is likely that one or both of these motifs are involved in the allosteric communication. Notably, the LptG β-jellyroll domain exhibits significant internal flexibility (Figure 5G-H). Biochemical studies showed that conserved residues in this domain are essential for cell growth20. Though there is little evidence for a direct role of this domain in LPS binding, the observed dynamics might be important for efficient transport. Similarly, in LptB2FG-C, the β-jellyrolls of LptC and LptF interact both in both apo and vanadate-trapped states (Figure 6A-B), which altogether might form a continuous pathway for LPS transfer towards LptA. The β-jellyrolls were not fully resolved in the vanadate-trapped structure of LptB2FG (Figure 1) and LptB2FGC23, revealing a considerable flexibility. The exact role for this enhanced dynamics is unclear and it may somehow facilitate LPS release. The narrow distance distributions we observed reveal that these domains might move as rigid bodies while sampling a broad conformational space.
The LILBID-MS data show that LPS is released upon ATP hydrolysis by LptB2FG (Figure 6H-J, Figure 7 steps 1-2). Thus, LptC is required for a productive transport cycle in the wild-type protein24,57,58. Our observations suggest that the TM-LptC dynamically associates and dissociates at the lateral gate-2, even in the apo-state while its β-jellyroll domain is firmly bound to LptF β-jellyroll (Figure 7, step 3). Such a dynamic behaviour of TM-LptC has previously been suggested based on the Klebsiella pneumoniae LptB2FGC structure, which showed density for the short N-terminus segment of TM-LptC59. Vanadate trapping induces the TM-LptC released conformation of the lateral gates (Figure 6C-D and F-G), in agreement with the structures, which also leads to LPS release20,22. Thus, ATP binding/hydrolysis might be coupled to the collapse of the TMDs and expulsion of LPS. Inferring from our observations on LptB2FG (Figure 5), ATP binding might allosterically open the LptF β-jellyroll (Figure 7, step 4), which might subsequently close after passing LPS to the LptC β-jellyroll to prevent any backflow20 (Figure 7, step 5). Through repeating this cycle, LptB2FG might push LPS along the trans-periplasmic bridge in a unidirectional manner according to the Pez candy dispenser model60. The possibility to observe LptB2FG and LptB2FGC offers further opportunity to characterize the dynamics of the β-jellyroll domains, LptB2FG – TM-LptC interaction as well as other intermediate states of the transport cycle even in the native-like lipid bilayers. These experiments, which are beyond the current scope of the study are in progress in our laboratory. As the persistently interacting, yet dynamic LptB2FGC complex forms the functional LPS transport unit in the inner membrane, a detailed understanding of its dynamics would facilitate development of novel drugs against Gram-negative bacterial pathogens.
Materials and Methods
Expression, purification and spin labeling of LptB2FG and LptB2FG-C
The plasmid pETDuet-lptB-lptFG, including a C-terminal His-tag on LptB, was used to transform in E. coli BL21(DE3) C43 for LptB2FG expression. For LptB2FGC a co-expression with the pCDFDuet-1-lptC together with pETDuet-lptB-lptFG was performed. For bacterial cell growth Luria broth (LB) medium with 100 µg/mL ampicillin (for LptB2FG, and additionally 50µg/mL streptomycin for LptB2FGC) was incubated at 37 °C until an optical density of 1.0 at 600 nm. The protein expression was induced with 0.2 mM or 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for LptB2FG and LptB2FGC, respectively. After overnight expression at 18 °C the cells were collected by centrifugation. Cell pellets were resuspended in lysis buffer (20 mM HEPES, pH 7.5, 300 mM NaCl) with a homogenizer and lysed by French press with an additional spatula tip of DNAse I, lysozyme and 100 µg/ml PMSF. Cell debris was removed by centrifugation at 12,000xg for 20 min at 4 °C. The membranes were pelleted by ultra-centrifugation at 200,000xg for 2 h at 4 °C. Membranes were flash-frozen in liquid nitrogen and stored at -80 °C. For solubilization lysis buffer with 1% (w/v) DDM was used and after homogenization membranes were incubated for 30 min at 4 °C. The protein solution was ultra-centrifugated at 100,000xg for 35 min at 4°C. The supernatant was incubated with washed Ni Sepharose (High Performance, GE Healthcare) for 45 min at 4 °C and loaded on an empty PD-10 column (GE Healthcare). The column was washed with 15 column volumes of wash buffer containing 20 mM HEPES, pH 7.5, 300 mM NaCl, 0.05% (w/v) DDM, 5% (v/v) glycerol and 40 mM imidazol. The protein bound to the Ni Sepharose was labeled with 2 column volumes of 20 mM HEPES, pH 7.5, 300 mM NaCl, 0.05% (w/v) DDM, 5% (v/v) glycerol and 300 µM MTSL (1-Oxyl-2,2,5,5-tetramethyl-3-pyrroline-3-methyl)methanethiosulfonate for 1.5 h at 4°C. The column was washed with 40 column volumes of the same buffer (without MTSL) and further eluted with 300 mM imidazole and immediately deslated using a PD-10 desalting column (GE Healthcare) into the same buffer. The protein was then concentrated to about 100 μM (Amicon Ultra-15, PLQK Ultracel-PL Membrane, 50 kDa, Merck, Millipore). After size-exclusion chromatography with a Superdex 200 Increase10/300 GL column (GE Healthcare), the protein fractions were collected and concentrated to ∼40 µM protein. For PELDOR spectroscopy different samples were prepared. For the ATP-EDTA sample, 5 mM ATP and 0.5 mM EDTA were added (5 min, 37 °C). The ADP-MgCl2 sample contained 5 mM ADP and 5 mM MgCl2 (1 min, RT). The vanadate-trapped sample was prepared with 5 mM ATP, 5 mM MgCl2 and 5 mM ortho-vanadate and the sample was incubated for 5-10 minutes at 37°C. All the samples were adjusted to the same final protein and DDM concentration (0.05%).
Proteoliposome reconstitution and PELDOR sample preparation
Liposomes (20 mg/mL, 20 mM HEPES, 300 mM NaCl, 10% (v/v) glycerol, pH 7.5) were mixed with 0.14% Triton-X and incubated for 30 min at room temperature. Liposomes and protein (in the same buffer additionally containing 5% glycerol and 0,05% (w/v) DDM) were mixed at 1:10 (w/w) ratio to a final lipid concentration of 4 mg/mL in 10 mL. The protein-lipid suspension was gently incubated for 1 h at room temperature. Afterwards Bio-Beads SM-2 (Bio-Rad) was added and was gently mixed for 30 min at room temperature and moved to 4º C. The Bio-Beads were added three more times for the following incubation periods at 4oC: 1 h, overnight and 2 h. Proteoliposomes were diluted to 2 % (v/v) glycerol in 20 mM HEPES, 300 mM NaCl, pH 7.5 and were pelleted at 200,000xg (4 °C) and resuspended into the same buffer. PELDOR samples were prepared as described above for DDM micelles with an additional freeze-thaw (5x) cycle. Samples were frozen in liquid nitrogen and stored at -80oC.
ATP assay
The ATP assay was performed according to a protocol modified from Morbach et al61. 5 μg of purified LptB2FG or LptB2FGC were incubated at 37 °C for 8 min in 25 µL reaction volume on a 96-well plate with different ATP concentrations (0, 0.125, 0.25, 0.5, 1, 2, 3 and 5 mM) in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.05 % (w/v) DDM and 5 mM MgCl2. The reaction was stopped with addition of 150 µL of 20 mM H2SO4. A 50 µL working solution [50 ml of ddH2O, 10 ml of ≥ 95 % H2SO4 and 73.4 mg of malachite green chloride, 40 μl of 11 % Tween 20, and 500 of μl 7.5 % (w/v) (NH4)6Mo7O24.4H2O solution] was added and incubated for 8 min at RT and the absorbance was measured at 620 nm in a microplate reader. The data was analyzed using Origin 2018.
Continuous wave (cw) ESR spectroscopy
Continuous wave ESR spectroscopy measurements were performed on a X-band Bruker EMXnano benchtop spectrometer. A 16 µL of the protein sample was measured in a 0.64 mm diameter micropipette (BRAND, Germany) with 100 kHz modulation frequency, 0.6-2 mW microwave power, 0.15 mT modulation amplitude and 18 mT sweep width.
Pulsed electron-electron double resonance spectroscopy
DEER/PELDOR experiments were conducted on a Bruker Elexsys E580 Q-Band (34 GHz) pulsed ESR spectrometer equipped with an arbitrary waveform generator (SpinJet AWG, Bruker), a 50 W solid-state amplifier, a continuous-flow helium cryostat, and a temperature control system (Oxford Instruments). Measurements were carried out at 50 K using a 10 – 20 µL frozen sample containing 15 – 20% glycerol-d8 in a 1.6 mm quartz ESR tube (Suprasil, Wilmad LabGlass) with a Bruker EN5107D2 dielectric resonator. The phase memory time (TM) measurements were performed with a 48 ns π/2–τ–π Gaussian pulse sequence with a two-step phase cycling after incrementing τ in 4 ns steps. A dead-time free four-pulse sequence with a 16-step phase cycling (x[x][xp]x) was used for DEER measurements62. A 38 ns Gaussian pump pulse (with a full width at half maximum (FWHM) of 16.1 ns) was employed, along with a 48 ns observer pulse (FWHM of 20.4 ns)63. The pump pulse was placed at the maximum of the echo-detected field swept spectrum, and the observer pulses were set 80 MHz lower. Deuterium modulations were averaged by progressively increasing the first interpulse delay by 16 ns over 8 steps. The data analysis was performed with the ComparativeDeerAnalyzer 2.0 (CDA)54,55 or the DeerLab program. The distance distributions were simulated on the structures (PDB 6MHU, 6MHX, 6MI7 and 6MI8) using a rotamer library approach as implemented in the MATLAB-based MMM2022.2 software package64.
Liquid bead ion desorption mass spectrometry (LILBID-MS)
For analysis by LILBID-MS, samples were buffer exchanged to 20 mM Tris(HCl), 0.05% DDM, pH 7.5. For ATP-Mg2+ sample, 2 mM ATP and 0.5 mM MgCl2 were added prior to the buffer exchange and the sample was incubated for 1 min at room temperature. After dilution to 10 µM protein, 4 µL of the sample were loaded directly into a droplet generator (MD-K-130, Microdrop Technologies GmbH, Germany). This piezo driven device generates droplets of a diameter of around 50 µm at a frequency of 10 Hz. The droplets are then transferred into vacuum, and irradiated by an IR laser working at a wavelength of 2.8 µm having a maximum energy output of 23 mJ per pulse (6 ns). The free ions were accelerated into a homebuilt time of flight analyser by applying an acceleration voltage in the Wiley McLaren type ion optics. The voltage between the first and the second lens was set to -4.0 kV. At 5 to 25 µs after irradiation, the first lens was pulsed to -6.6 kV for 370 µs, while the reflectron worked at -7.2 kV. Detection was conducted by a homebuilt Daly type detector, optimized for high m/z.
Acknowledgements
This work was financially supported from the Deutsche Forschungsgemeinschaft via the Emmy Noether program (JO 1428/1−1), SFB 1507 − “Membrane-associated Protein Assemblies, Machineries, and Supercomplexes”, and a large equipment fund (438280639) to B.J. N.M. acknowledges funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) — Project-ID 426191805. B.J. and M.D. thanks Jingyi Liu for establishing LptB2FGC purification and PELDOR experiments.
Data Availability
The PELDOR/DEER data are available upon request to B.J.
Supplementary material
Note
This reviewed preprint has been updated to move the supplementary material to the end of the article.
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