Validation of the inhibition of bacterial growth caused by the suppression of the expression of TnSeq-hit genes using the CRISPR-i system.
Open bar: day 3; closed bar: day 7. a Comparative growth rates of the knockdown strains relative to those of the vector control strains in the representative universal essential or growth-defect-associated genes: glcB, inhA, gyrB and embB. b Comparative growth rates of the knockdown strains relative to those of the vector control strains in the representative accessory and strain-dependent essential or growth-defect-associated genes: pckA, glpX, csd and ESX-5 type VII secretion components. Data are shown as the means ± SD of triplicate experiments. Data from one experiment representative of two independent experiments (N = 2) are shown.