(A) Food intake, (B) physical activity, including total horizontal and ambulatory activity, (C) respiratory exchange ratio (VCO2/VO2) and (D) oxygen consumption (VO2) data for 30-month-old male wild-type (WT) and FGF21-transgenic (Tg) mice (n=6/group) housed singly in metabolic cages. For (D), the area under the curve (AUC) data are shown in the right panel. (E) Body weights of male and female mice measured at 6, 12 and 26 months. Measurements were done on all surviving mice in the cohorts (n=27–54/group). (F) Fat and lean mass percentages were measured in 26-month-old mice (n=5–6/group). (G) Adiponectin oligomer forms, including high molecular weight (HMW), medium molecular weight (MMW) and low molecular weight (LMW) forms, were measured in plasma from 26- to 27-month-old male and female WT and FGF21-Tg mice. Representative western blots using plasma from single animals are shown together with the ratios of the different adiponectin forms in FGF21-Tg and WT plasma (n=4/group). (H) Quantification of trabecular bone volume by μCT analysis using tibiae from 33- to 35-month-old male WT and FGF21-Tg mice (n=4–5/group). All data are presented as the mean ± SEM.