The ART-Rsp5 ubiquitin ligase network comprises a plasma membrane quality control system that protects yeast cells from proteotoxic stress
- Cornell University, United States
Figures
Figure 1 with 10 supplements
Heat stress triggers endocytic downregulation.
(A) Fluorescence distribution of GFP-tagged endocytic cargoes (green) was analyzed in wildtype yeast cells expressing the vacuolar marker Vph1-mCherry (red). Cells were grown to mid-log phase at …
Figure 1—figure supplement 1
Cell surface fluorescence intensity was measured for Mup1-GFP and Can1-GFP following growth at 26°C or following a shift to 38°C for 2 hr.
Top panels illustrate how PM fluorescence intensity was measured and calculated and bottom panels depict fluorescence intensity measurements over many cells (n > 30 cells).
Figure 1—figure supplement 2
Mup1-pHluorin was used to quantify surface abundance of Mup1 for a population of yeast cells at 26°C or following a shift to 38°C for 2 hr (bottom panel).
Mup1 pHluorin signal originates almost exclusively from the PM (top panel).
Figure 1—figure supplement 3
Stability of affinity-tagged Mup1 and Pma1 was analyzed following temperature shift from 26°C to indicated temperatures.
https://doi.org/10.7554/eLife.00459.006
Figure 1—figure supplement 4
Stability of Lyp1 was analyzed following temperature shift from 26°C to indicated temperatures in the presence or absence of 3% glycerol.
https://doi.org/10.7554/eLife.00459.007
Figure 1—figure supplement 5
Stability of affinity-tagged Aqr1 and Pdr5 was analyzed following temperature shift from 26°C to indicated temperatures.
https://doi.org/10.7554/eLife.00459.008
Figure 1—figure supplement 6
Identification of integral PM proteins induced by shifting cells to 40°C.
https://doi.org/10.7554/eLife.00459.009
Figure 1—figure supplement 7
Various proteotoxic stresses trigger endocytic downregulation.
Fluorescence distribution of GFP-tagged endocytic cargoes (green) was analyzed in wildtype yeast cells expressing the vacuolar marker Vph1-mCherry (red). Cells were grown to mid-log at 26°C and then …
Figure 1—figure supplement 8
Analysis of cargo thermostability.
For each heat-induced degradation timecourse experiment, the half-life of each cargo was estimated at each temperature using linear regression. Cargo half-life is shown plotted as a function of …
Figure 1—figure supplement 9
Analysis of Lyp1 thermostability in the absence or presence of 3% glycerol.
See also Figure 1—figure supplement 2.
Figure 1—figure supplement 10
Analysis of the kinetics of Mup1 lysosomal degradation following the addition of methionine at 26°C in the absence or presence of 3% glycerol.
https://doi.org/10.7554/eLife.00459.013
Figure 2
Rsp5 mediates the heat-induced endocytic response.
(A) Fluorescence distribution of GFP-tagged endocytic cargoes (green) was analyzed in wildtype (left panels), rsp5-ww2 (middle panels), or rsp5-ww3 (right panels) yeast cells expressing the vacuolar …
Figure 3
Rsp5-mediated endocytic clearance protects PM integrity during heat stress.
(A) Yeast cells that were either grown at 26°C to mid-log phase (left), heated to 65°C for 10 min (middle), or treated with nystatin (right) were stained with propidium iodide (PI) and analyzed by …
Figure 4 with 3 supplements
ARTs protect plasma membrane integrity during heat stress.
(A) Heat-sensitivity analysis of wildtype and art mutant yeast cells. See Figure 4—figure supplement 1 for additional characterization of art mutant yeast cells. (B) Fluorescence distribution of …
Figure 4—figure supplement 1
Heat-sensitivity analysis of wildtype and art mutant yeast cells.
https://doi.org/10.7554/eLife.00459.018
Figure 4—figure supplement 2
Fluorescence distribution of GFP-tagged Art1 (green) was analyzed at 26°C and 38°C in yeast cells expressing the vacuolar marker Vph1-mCherry (red).
Cells were grown to mid-log at 26°C and then shifted to 38°C for 2 hr. Plasma membrane (‘PM’) and Golgi localization are indicated.
Figure 4—figure supplement 3
Results of a screen to identify multicopy bypass suppressors of the Δart1 ts phenotype.
Overexpression of ART2 can suppress the ts phenotype but not the canavanine hypersensitivity phenotype of Δart1 mutant cells.
Figure 5 with 3 supplements
Cargo accumulation at the PM is toxic during heat stress.
(A) heat-sensitivity analysis of wildtype, Δart1, Δlyp1, and Δart1Δlyp1 mutant yeast cells. (B) Empty vector or plasmids encoding Lyp1-FLAG expressed from different promoters (pCPY, pLYP1, pADH1) …
Figure 5—figure supplement 1
Heat-sensitivity analysis of wildtype yeast cells expressing titrated levels of Lyp1.
https://doi.org/10.7554/eLife.00459.022
Figure 5—figure supplement 2
Stability of wildtype Lyp1 or the K10XR mutant, which cannot be ubiquitinated on its N-terminal cytosolic tail.
https://doi.org/10.7554/eLife.00459.023
Figure 5—figure supplement 3
Fluorescence distribution of GFP-tagged wildtype Lyp1 (green, only in cells expressing Vph1-mCherry) or the K10XR mutant (green, only in cells without Vph1-mCherry) was analyzed following growth at 38°C for 2 hr.
https://doi.org/10.7554/eLife.00459.024
Figure 6 with 1 supplement
Systems level coordination of integral membrane protein quality control.
(A) Model illustrating the major quality control mechanisms for integral membrane proteins: ERAD (proteasomal degradation), Golgi quality control (GQC; vacuolar/lysosomal degradation), and plasma …
Figure 6—figure supplement 1
Heat-sensitivity analysis of wildtype and mutant yeast cells.
https://doi.org/10.7554/eLife.00459.026
Figure 7
Cellular mechanisms of integral membrane protein quality control.
Integral membrane proteins are subject to sequential quality control mechanisms including ERAD in the ER (I), GQC in the Golgi (II), and ART-Rsp5 mediated PMQC at the PM (III).
Tables
Table 1
Cargo thermostability
| Cargo | 34°C | 38°C | 40°C | 42°C |
| Lyp1 | 160 | 50 | 25 | – |
| Pma1 | Stable | 95 | 45 | – |
| Mup1 | Stable | 100 | 65 | – |
| Aqr1 | – | Stable | 100 | 70 |
| Pdr5 | – | Stable | 400 | 200 |
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Kinetic analysis of heat-induced cargo degradation (Figure 1—figure supplements 3 and 4) was used to estimate half-lives of each cargo at each temperature tested. Half-lives are indicated in minutes. ‘–’ indicates half-life not determined.
