Aggregation was induced in different stained cell populations (‘Materials and methods’). (A) Left, population of cells stained with Lysotracker (green), uniformly green aggregates are formed. Center, population of cells stained with Chormeo Mitochondrial Staining (cyan), uniformly cyan aggregates are formed. Right, two independently stained populations of cells (green or cyan) are mixed and dual color aggregates are formed, indicating that cells from different origins aggregate to each other. (B) Total number of cells per day in control cells, aphidicolin-treated cells and hydroxyurea-treated cells. Note that cell division is blocked by both aphidicolin and hydroxyurea. Aggregate formation was evaluated under each condition. All cells, even those treated with aphidicolin or hydroxyurea, developed aggregates. A representative aggregate is shown for each condition. Scale bars= 10 μm.