(A) 1-day-old sir-2.1(0) and 2-day-old wild-type males have more lipid content than 1-day-old wild type. Left: quantification of fat staining, mean ± SEM, unpaired t-test. Right: representative images of fat staining. (B) Mating potency of wild-type and sir-2.1(0) males. Copulations were allowed to occur for 5 hr (i) or for an unlimited time (ii). The number of males in each assay is listed at the bottom of each bar. The numerical percentage of wild-type males that mated on day 1 was normalized to 100%. The normalization factor was then applied to the other experimental conditions. The normalized percentages for each day are listed on the top. Fisher’s exact test was used to compare the mating potency prior to normalization. (C) Transient starvation reduces sir-2.1(0) mating deficiency. (D) Mating potency of sir-2.1(0) and rescued strain sir-2.1(0); rgEX399 [Psir-2.1:sir-2.1::yfp]. ns, not significant. Asterisks *, ** and *** indicate the p<0.05, 0.01, and 0.0001 in this paper, respectively.