(A) Helix pomatia lectin (HPL) staining was analysed using the ‘Transfluor HT’ module of MetaXpress software (Molecular Devices). A mask was generated for both HPL and nuclei (Hoechst) staining to …
(A) Comparison of the HPL intensities between the two screen replicates. (B) Comparison of HPL intensities generated from different analysis algorithms from the HCSU (high content screening unit) …
(A) Peanut Agglutinin (PNA) lectin staining in ERK8-depleted HeLa cells. Scale bar: 30 μm. (B) PNA lectin staining quantification after depletion of the 12 Tn regulators, using COSMC knockout HeLa …
(A) Peanut Agglutinin (PNA) lectin staining quantification after depletion of the 12 Tn regulators with a single siRNA from the pool used in the screen and in COSMC knockout cells. (B) Pearson's …
(A) Protein replacement by expression of siRNA-resistant wild-type ERK8 or the kinase inactive mutant in ERK8-depleted HeLa cells. Cells were stained for Helix pomatia lectin (HPL) and ERK8. Scale …
(A) Tn staining with Helix Pomatia Lectin (HPL) and quantification of cells expressing mirRNA (Invitrogen) for ERK8. Scale bar: 10 μm. (B) SDS-PAGE analysis of ERK8 expression levels in cells …
(A) High magnification of HeLa MannII-GFP expressing cells stained for endogenous ERK8 following cytosol extraction. (B) Cytosol-depleted cells treated with platelet-derived growth factor (PDGF; 50 …
(A) MannII-GFP- and Src-8A7F-mcherry-expressing cells were treated with imidazole for the indicated times, cytosol-depleted and stained for ERK8. Scale bar: 10 μm. (B) Pearson's correlation …
(A) Co-knockdown of ERK8 with Arf1 or GTP exchange factor, GBF1, and staining with Helix pomatia lectin (HPL). NT, non-targeting. Scale bar: 30 μm. (B) Quantification of Tn levels upon ERK8 …
(A) Tn expression levels, as determined by Helix Pomatia Lectin (HPL) staining in cells expressing dominant-negative mutant Arf1 (Q71L) compared to wild-type Arf1. GRASP55 labels the Golgi …
(A) Phase contrast images and (B) actin and tubulin staining of non-targeting (NT) siRNA-treated and ERK8-depleted cells. Scale bars: 100 μm in (A) and 10 μm in (B). (C) Migration assay using …
(A) Cell-surface staining using a specific Tn antibody (green) and Helix Pomatia Lectin (HPL) (red) on non-permeabilised ERK8-depleted cells. Scale bar: 10 μm. (B) Scratch wound assay of cellular …
(A) Quantification of ERK8 staining in human breast biopsies. Each point represents the staining of one tissue core normalised to the average staining of the normal tissue cores. (B) Quantification …
(A) H&E staining of 40 breast biopsies on BRF404 slides purchased from US Biomax, Inc. Image is from Biomax website (http://www.biomax.us/). (B) ERK8 and (C) Vicia Villosa Lectin (VVL) were …