Phylogeny was adapted from Trautwein et al. (2012). Timescale was adjusted for higher level taxa based on Rota-Stabelli et al. (2013), for Holometabola according to Wiegmann et al. (2009) and the …
Animals are depicted next to the corresponding antennal SEM images. (A) Detailed view of the antennae of L. y-signata. The proximal part of the antennae is not only covered with sensilla, but also …
Spikes are sorted by neurons, with the exception of ac1, ac2, and ac4 of D. melanogaster where spike sorting was not possible. Means over 5 to 23 recordings were used as basis for visualization …
Excel file of mean responses and baseline firing rate of the different OSN classes of L.y-signata, T. domestica, P.siccifolium, and D. melanogaster.
(A) Dendrogram displaying the relationship of identified OR and GR candidates of L. y-signata, T. domestica, and P. siccifolium to D. melanogaster (Clyne et al., 1999; Gao and Chess, 1999; Vosshall …
Amino acid sequences of putative olfactory and gustatory receptors of L. y-signata, T. domestica, and P. siccifolium.
Nucleotide sequences of putative olfactory and gustatory receptors of L. y-signata, T. domestica, and P. siccifolium.
MAFFT-alignment of OR and GR candidates of L. y-signata, T. domestica, P. siccifolium and D. melanogaster (Clyne et al., 1999, Gao and Chess, 1999, Vosshall et al., 1999) and Apis mellifera (Robertson and Wanner, 2006) GR and OR proteins, as well as Daphnia pulex GRs done.
FastTree file resulting from the MSA of Figure 4—source data 3 (can be opened with FigTree).
Tree file resulting from the MSA of Figure 4—source data 3 containing node support values (can be opened e.g., with Adobe Illustrator).
Alignment of T. domestica Orcos with Orcos of Acyrthosiphon pisum (GI:328723530), A. mellifera (GI:201023349), D. melanogaster (GI:24644231), Schistocerca gregaria (GI:371444780), Pediculus humanus …
Using RT-PCR Orco expression was detected in the antennae (A) of T. domestica, but not in legs (L), heads without antennae and palps (H), and bodies (B). Primer sequences are given in Figure …
Primers and their properties used in this study.
(A) Part of a T. domestica antenna. Combined image of fluorescent and transmitted light channel taken with cLSM. The positions of pored sensilla are indicated by arrowheads, with the upper sensillum …
(A) Transmitted light images taken with cLSM. The position of olfactory sensilla is indicated by arrowheads. (B–E) Projection section through the antennae. No Dig signals were obtained using the …
(A) Analysis of the relationship between L. y-signata, T. domestica, P. siccifolium, D. melanogaster and D. pulex iGluRs and IRs (D. melanogaster and D. pulex sequences were sequences taken from Cros…
Amino acid sequences of putative variant ionotropic glutamate receptors of L. y-signata, T. domestica, and P. siccifolium.
Nucleotide sequences of putative variant ionotropic glutamate receptors of L. y-signata, T. domestica, and P. siccifolium.
MAFFT amino acid alignment of iGluR and IR candidates of L. y-signata, T. domestica, P. siccifolium, D. melanogaster, and D. pulex (D. melanogaster and D. pulex sequences were sequences taken from Croset et al., 2010).
FastTree file resulting from the MSA of Figure 4—source data 3 (can be opened with FigTree).
Tree file resulting from the MSA of Figure 8—source data 3 containing node support values.
On the left: schematic drawings of the position of the different sensillum types on the particular antennal subsegment. The legend for the sensillum types is given below the confocal images. (A–D) …
On the left: schematic drawings of the position of the different sensillum types on antennae. The legend for the sensillum types is given below the confocal images. (A–I) Labeling of somata in a …
(A, D, G) Transmitted light pictures of antennal excerpts. Asterisks mark positions of basiconic sensilla, arrowheads places were coeloconic-like sensilla are located. No signals were obtained in …
Technical overview of transcriptomes (study accession: PRJEB5093, study unique name: ena-STUDY-MPICE-12-12-2013-15:03:23:860-31)
Organism | Sequencing technique | Number of reads | Number of contigs above 400 bp | N50 | Average length of contigs | Tissue | Sample accession | Secundary accession | Sample unique name |
---|---|---|---|---|---|---|---|---|---|
Lepismachilis y-signata | HiSeq2000 (Illumina) | 22’444’128 | 68’984 | 1’179 | 1’000 | antennae and palps | ERS384175 | SAMEA2276780 | Lysig1 |
HiSeq2500 (Illumina) | 77’060’687 paired end | antennae | ERS384176 | SAMEA2276781 | Lysig2 | ||||
HiSeq2000 (Illumina) | 25’242’666 | 37’860 | 857 | heads, whole bodies | ERS399748 | SAMEA2342071 | LysigMix1 | ||
Thermobia domestica | HiSeq2500 (Illumina) | 27’704’231 paired end | 31’172 | 1’349 | 1’070 | antennae | ERS384177 | SAMEA2276782 | Tdom1 |
Phyllium siccifolium | HiSeq2500 (Illumina) | 30’762’777 paired end | 34’653 | 1’890 | 1’305 | antennae | ERS384178 | SAMEA2276783 | Psic1 |
Number of candidate contigs (not unigenes) for the different gene families identified in the transcriptomes of the different species
Organism | Orco | ORs | GRs | IRs |
---|---|---|---|---|
Lepismachilis y-signata | – | – | 7 (5 above 400 bp) | 17 (16 above 400 bp) |
Thermobia domestica | 6 (1 above 400 bp) | – | 9 (3 above 400 bp) | 19 (9 above 400 bp) |
Phyllium siccifolium | 1 (1 above 400 bp) | 30 (16 above 400 bp) | 6 (2 above 400 bp) | 32 (19 above 400 bp) |