(A and B) Confocal images of animals stained for FoxA, myosin (muscle), PC2 (neurons), α-acetylated tubulin (epithelial cells) and nuclei showing FoxA enrichment in epithelial cells 3 days after pharynx amputation, and shifting to mesenchyme 7 days after amputation. (C) Confocal images of Smedwi-1 FISH (7 days after pharynx amputation) showing distribution of stem cells. (D) Body-wide phosphoH3-Ser10 staining in FoxA(RNAi) animals during pharynx regeneration. Error bars = SD. (E) Local phosphoH3-Ser10 staining during pharynx regeneration in FoxA(RNAi) animals. Error bars = SD. (F) Dorsal outgrowths in FoxA(RNAi) animals (day 20) lack pharyngeal tissue. Tissue-specific markers include: laminin, npp-1 (pharynx), porcupine (intestine), ndk, PC2 (neurons), collagen (muscle). In all images, anterior is up; in side views, dorsal is to the right. Scale bars (A and B) 50 μm, (C) 500 μm, (F) 250 μm.