(A) Schematic of breeding for the analysis of Camk2a-cre expression analysis in B–D. (B–D) Representative images of cre-dependent tdTomato expression in (B) medial pre-frontal cortex (mPFC), (C) anterior cingulate cortex (ACC), and (D) Hippocampus (Hip). (E–G) Representative images of golgi stained tissue sections from (E) the mPFC, (F) the ACC, and (G) CA1 hippocampal region. (H–J) Representative images of individual dendritic segments from the (H) the mPFC, (I) ACC, or (J) CA1 hippocampal region (top panels) control Cdc42f/f or (bottom panels) cKO Cdc42f/f: Camk2a-Cre mice. (K–M) Graphs depicting the quantitative analysis of spines per 100 micron of dendritic seqements for each genotype from each region in H–J. n = 5 for each group. *p<0.05.