(A) Lipid dequenching reactions of 1R- and 3Q-RPLs (250 µM lipid each), with both fluorescent lipids (Marina-Blue-phosphatidylethanolamine [MB-PE] and NBD-PE, 1.5 mol% each) on either the 1R-RPLs or the 3Q-RPLs. Incubations contained either Sec17p (600 nM) and Sec18p (200 nM) (light gray) or no additional proteins (dark gray). (B) Lipid dequenching reactions of MB/NBD-labeled 1R- (light gray) or 3Q-RPLs (dark gray) with increasing amounts of non-labeled complementary RPLs (3Q or 1R; 1:1 corresponds to 50 µM lipid of each). No additional proteins were added. (C) Sensitivity of lipid dequenching reactions of 1R(MB/NBD)-RPLs and 3Q-RPLs to various inhibitors that might prevent trans-SNARE complex formation. Incubations received: (1) no addition, (2) Sec17p/Sec18p (400 nM each), (3) antibodies directed against Vam3p (1 µM), or (4) soluble Nyv1p(1-231) (1 µM). Column 5: 1R(MB/NBD)-RPLs were incubated with non-fluorescent RPLs bearing various combinations of SNAREs other than 3Q: QabcR, QbcR, QacR, QabR, QcR, QbR, Qbc, QaR, Qac, Qab, R, Qc, Qb, Qa. The extent of these reactions was comparably low for all conditions, and is shown as average for these 14 conditions with standard deviations.