Biochemistry and Chemical Biology

Biochemistry and Chemical Biology

eLife publishes research that gives new insights into biological molecules or uses chemical approaches to illuminate complex biological processes. Learn more about what we publish and sign up for the latest research.
Illustration by Davide Bonazzi

Latest articles

    1. Biochemistry and Chemical Biology

    High-throughput proteomics of nanogram-scale samples with Zeno SWATH MS

    Ziyue Wang, Michael Mülleder ... Markus Ralser
    Zeno SWATH MS, a novel data-independent acquisition technique in proteomics, which by facilitating sensitive detection in high-throughput experiments with low sample amounts, mitigates current bottlenecks of high-throughput proteomics.
    1. Biochemistry and Chemical Biology
    2. Neuroscience

    Chronic Ca2+ imaging of cortical neurons with long-term expression of GCaMP-X

    Jinli Geng, Yingjun Tang ... Xiaodong Liu
    Focusing on calcium oscillations in relation to neuronal morphology both in vitro and in vivo, rationally designed GCaMP-X provides a simple solution to neuronal toxicity of conventional GCaMP in imaging applications involving prolonged and/or strong expression of calcium probes.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Integrative modeling reveals the molecular architecture of the intraflagellar transport A (IFT-A) complex

    Caitlyn L McCafferty, Ophelia Papoulas ... Edward M Marcotte
    The 3D structure of the six-subunit complex and its polymeric assembly gives insights into cargo transport in cilia and how specific mutations in these genes lead to ciliopathy birth defects.
    1. Biochemistry and Chemical Biology

    DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic function

    Joshua Jeong, Joyce H Lee ... James M Berger
    The ability of eukaryotic topoisomerase IIs to undergo liquid-liquid phase separation is a novel process for this class of enzymes and may provide answers to many unexplained observations found in both in vitro and in vivo studies.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Molecular basis of the PIP2-dependent regulation of CaV2.2 channel and its modulation by CaV β subunits

    Cheon-Gyu Park, Wookyung Yu, Byung-Chang Suh
    The anchoring properties of CaV β2 subunits to the plasma membrane determine the biophysical states of CaV2.2 channels by regulating PIP2 coupling to the nonspecific phospholipid-binding site in the I–II loop.
    1. Biochemistry and Chemical Biology

    Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control

    Meiling Wu, Anda Zhao ... Dongyun Shi
    Moderate exercise upregulated compensatory antioxidant capability and reached a high-level redox balance, whereas antioxidant intervention achieved a low-level redox balance by inhibiting oxidative stress for treating diabetes.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Molecular mechanism of Afadin substrate recruitment to the receptor phosphatase PTPRK via its pseudophosphatase domain

    Iain M Hay, Katie E Mulholland ... Janet E Deane
    Substrate recognition by a receptor protein tyrosine phosphatase is mediated by binding to its pseudophosphatase domain via a short helix that is >100 amino acids distant from the target phosphosite, uncovering principles of phosphatase substrate recognition and potential scaffolding functions.

Senior editors

  1. Anna Akhmanova
    Utrecht University, Netherlands
  2. Nancy Carrasco
    Vanderbilt University School of Medicine, United States
  3. José Faraldo-Gómez
    National Institutes of Health, United States
  4. See more editors