Biochemistry and Chemical Biology

Latest articles

1. 1. Biochemistry and Chemical Biology
   2. Cell Biology

   An ER translocon for multi-pass membrane protein biogenesis

   Philip T McGilvray et al.

   A set of ER-localized membrane proteins whose loss causes developmental diseases in humans, assemble with Sec61 into a translocon that facilitates the biogenesis of hundreds of different multi-pass membrane proteins.
2. 1. Biochemistry and Chemical Biology
   2. Structural Biology and Molecular Biophysics

   Serine phosphorylation regulates the P-type potassium pump KdpFABC

   Marie E Sweet et al.
3. 1. Biochemistry and Chemical Biology

   A novel DNA primase-helicase pair encoded by SCCmec elements

   Aleksandra Bebel et al.
4. 1. Biochemistry and Chemical Biology

   Alkylative damage of mRNA leads to ribosome stalling and rescue by trans translation in bacteria

   Erica N Thomas et al.
5. 1. Biochemistry and Chemical Biology
   2. Cell Biology

   Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein

   Mikel Garcia-Marcos et al.
6. 1. Biochemistry and Chemical Biology

   Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3

   James E Collier et al.

   Rigorous biochemical and structural analyses reveal the precise topology of cohesin's association with DNA and suggest a mechanism for how DNA is transported inside the ring.
7. 1. Biochemistry and Chemical Biology
   2. Neuroscience

   Erasable labeling of neuronal activity using a reversible calcium marker

   Fern Sha et al.

   Protein engineering of a reversibly switchable fluorescent protein enables post-hoc reversible and repeatable marking of elevated calcium concentrations using blue light.
8. 1. Biochemistry and Chemical Biology
   2. Cell Biology

   Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes

   Benjamin D Hobson et al.

   Although puromycin staining is often used to examine subcellular translation, puromycin-labeled proteins are rapidly released from ribosomes even in the presence of elongation inhibitors, which may confound translation site localization.
9. 1. Biochemistry and Chemical Biology
   2. Cell Biology

   Puromycin reactivity does not accurately localize translation at the subcellular level

   Syed Usman Enam et al.

   Methods that use puromycin to localize active translation do not actually detect the site of translation.
10. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    EDF1 coordinates cellular responses to ribosome collisions

    Niladri K Sinha et al.

    Endothelial Differentiation Factor 1 (EDF1) plays a critical role in driving mRNA-specific quality control and global transcriptional responses in response to ribosome collisions.