By random nonstandard peptide integrated discovery, combinatorial macrocyclic peptides were leavaged that target a heterodimeric ABC transport complex and explore fundamental principles of the substrate translocation cycle.
Despite the known heme attachment motif (CXXCH) in all c-type cytochromes, attachment elements recognized by human and bacterial cytochrome c biogenesis pathways are distinct, providing clear targets for differential inhibitors.
Biochemical analysis and computational modeling reveal how cells mechanistically control the quality of their proteomes and demonstrate that the precise alignment of subunits in oligomeric complexes can profoundly affect enzymatic properties.
A real-time, stopped-flow method, in combination with protein-induced fluorescence enhancement, Förster resonance energy transfer, and time-resolved fluorescence spectroscopy, reveals a kinetic framework for understanding Dicer as a complex molecular motor.
The general transcription elongation factor NusG functions as an intrinsic termination factor in Bacillus subtilis and together with NusA coordinates global gene expression including the motility regulon.