Biochemistry and Chemical Biology

Latest articles

1. 1. Biochemistry and Chemical Biology

   High-throughput proteomics of nanogram-scale samples with Zeno SWATH MS

   Ziyue Wang, Michael Mülleder ... Markus Ralser

   Zeno SWATH MS, a novel data-independent acquisition technique in proteomics, which by facilitating sensitive detection in high-throughput experiments with low sample amounts, mitigates current bottlenecks of high-throughput proteomics.
2. 1. Biochemistry and Chemical Biology
   2. Neuroscience

   Chronic Ca²⁺ imaging of cortical neurons with long-term expression of GCaMP-X

   Jinli Geng, Yingjun Tang ... Xiaodong Liu

   Focusing on calcium oscillations in relation to neuronal morphology both in vitro and in vivo, rationally designed GCaMP-X provides a simple solution to neuronal toxicity of conventional GCaMP in imaging applications involving prolonged and/or strong expression of calcium probes.
3. 1. Biochemistry and Chemical Biology
   2. Chromosomes and Gene Expression

   N6-methyladenosine (m6A) reader Pho92 is recruited co-transcriptionally and couples translation to mRNA decay to promote meiotic fitness in yeast

   Radhika A Varier, Theodora Sideri ... Folkert Jacobus van Werven
4. 1. Biochemistry and Chemical Biology
   2. Cancer Biology

   Collateral deletion of the mitochondrial AAA+ ATPase ATAD1 sensitizes cancer cells to proteasome dysfunction

   Jacob M Winter, Heidi L Fresenius ... Jared Rutter
5. 1. Biochemistry and Chemical Biology
   2. Structural Biology and Molecular Biophysics

   Integrative modeling reveals the molecular architecture of the intraflagellar transport A (IFT-A) complex

   Caitlyn L McCafferty, Ophelia Papoulas ... Edward M Marcotte

   The 3D structure of the six-subunit complex and its polymeric assembly gives insights into cargo transport in cilia and how specific mutations in these genes lead to ciliopathy birth defects.
6. 1. Biochemistry and Chemical Biology

   DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic function

   Joshua Jeong, Joyce H Lee ... James M Berger

   The ability of eukaryotic topoisomerase IIs to undergo liquid-liquid phase separation is a novel process for this class of enzymes and may provide answers to many unexplained observations found in both in vitro and in vivo studies.
7. 1. Biochemistry and Chemical Biology

   Discovery of a new class of reversible TEA-domain transcription factor inhibitors with a novel binding mode

   Lu Hu, Yang Sun ... Xu Wu
8. 1. Biochemistry and Chemical Biology
   2. Cell Biology

   Molecular basis of the PIP₂-dependent regulation of Ca_V2.2 channel and its modulation by Ca_V β subunits

   Cheon-Gyu Park, Wookyung Yu, Byung-Chang Suh

   The anchoring properties of Ca_V β2 subunits to the plasma membrane determine the biophysical states of Ca_V2.2 channels by regulating PIP₂ coupling to the nonspecific phospholipid-binding site in the I–II loop.
9. 1. Biochemistry and Chemical Biology

   Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control

   Meiling Wu, Anda Zhao ... Dongyun Shi

   Moderate exercise upregulated compensatory antioxidant capability and reached a high-level redox balance, whereas antioxidant intervention achieved a low-level redox balance by inhibiting oxidative stress for treating diabetes.
10. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Molecular mechanism of Afadin substrate recruitment to the receptor phosphatase PTPRK via its pseudophosphatase domain

    Iain M Hay, Katie E Mulholland ... Janet E Deane

    Substrate recognition by a receptor protein tyrosine phosphatase is mediated by binding to its pseudophosphatase domain via a short helix that is >100 amino acids distant from the target phosphosite, uncovering principles of phosphatase substrate recognition and potential scaffolding functions.