(A–F) Proliferating NB3-3A contains nuclear Deadpan (A), cytoplasmic Mira (B), nuclear Asense (C), nuclear Worniu (D), and nuclear CycE (E) at embryonic stages 14 and 16; quantification is shown in …
(A) The newly hatched larval (NHL) central brain contains five persistently proliferative neuroblasts (the four mushroom body neuroblasts and the lateral antennal lobe neuroblast) and ∼100 quiescent …
No significant change in the number of proliferating NB3-3T in stage 15 embryos with overexpressed Asense (AseOXN) or Cyclin E (CycEOXN). Error bars, standard deviation; n.s., not significant. Fly …
(A–B) NB3-3T shows transient nuclear Prospero (Pros) during entry into quiescence at stage 15 (S15) (B). Differentiated daughter cell shows strong nuclear Prospero and is indicated by the arrow. …
(A) Confocal images of a wild type NB3-3T (blue circle, identified by Eg-GFP and Dpn) and its adjacent GMC (yellow circle, identified by Eg-GFP and its smaller size) in stage 15 embryos. Scale bar: …
(A–B) Wild type and prospero mutant (pros17) stage 16 embryos. Parental neuroblasts (NB) stain for Deadpan and mγ-LacZ, whereas ectopic ‘de-differentiated’ neuroblasts stain for Deadpan but not …
(A–C) Transient overexpression of nuclear Prospero in neuroblasts (worniu-gal4 UAS-HA:prospero tub-gal80ts) results in neuroblast quiescence (A) or differentiation (B). Third-instar larvae were …
(A) Two levels of Prospero were generated by increasing time of misexpression (4 or 12 hr) with one copy of UAS-HA:prospero transgene. Whereas 2 hr can induce neuroblast quiescence (Figure 4), 4 hr …
(A–G) Transient overexpression of nuclear Prospero in neuroblasts (worniu-gal4 UAS-HA:prospero tub-gal80ts) induces neuroblast cell cycle arrest. Embryos were shifted from 22°C to 30°C for 1 hr to …