(A–F) Proliferating NB3-3A contains nuclear Deadpan (A), cytoplasmic Mira (B), nuclear Asense (C), nuclear Worniu (D), and nuclear CycE (E) at embryonic stages 14 and 16; quantification is shown in (F). Neuroblast lineages are marked by Eg-GFP (Eg-gal4 UAS-mCD8:GFP), and the neuroblast is identified by Dpn (shown in insets) and indicated by arrows. Anterior side is up, and lateral is at right. Scale bar: 5 μm. (G–L) Proliferating NB3-3T expresses all above-mentioned neuroblast markers at stage 14 (S14). At stage 16 (S16), NB3-3T is quiescent and contains Deadpan (G) and Mira (H) but lacks Asense (I), Worniu (J) and CycE (K). Quantified in (L). Scale bar: 5 μm. (F and L) Quantification; number of neuroblasts scored shown in bar. (M) Schematic summary of neuroblast marker profiles of proliferating neuroblast, quiescent neuroblast and neuron.