Transient nuclear Prospero induces neural progenitor quiescence
- Howard Hughes Medical Institute, University of Oregon, United States
- University of Oregon, United States
Figures
Figure 1 with 2 supplements
Quiescent neuroblasts have a novel transcriptional profile lacking both progenitor and differentiation markers.
(A–F) Proliferating NB3-3A contains nuclear Deadpan (A), cytoplasmic Mira (B), nuclear Asense (C), nuclear Worniu (D), and nuclear CycE (E) at embryonic stages 14 and 16; quantification is shown in …
Figure 1—figure supplement 1
Larval quiescent neuroblasts are Deadpan-positive but lack the progenitor markers Miranda, Asense, Cyclin E, Worniu and the differentiation markers Prospero, Elav, and Repo.
(A) The newly hatched larval (NHL) central brain contains five persistently proliferative neuroblasts (the four mushroom body neuroblasts and the lateral antennal lobe neuroblast) and ∼100 quiescent …
Figure 1—figure supplement 2
Overexpressing Asense and Cyclin E does not change the timing of neuroblast quiescence.
No significant change in the number of proliferating NB3-3T in stage 15 embryos with overexpressed Asense (AseOXN) or Cyclin E (CycEOXN). Error bars, standard deviation; n.s., not significant. Fly …
Figure 2 with 1 supplement
Transient low level nuclear Prospero is tightly correlated with neuroblast entry into quiescence.
(A–B) NB3-3T shows transient nuclear Prospero (Pros) during entry into quiescence at stage 15 (S15) (B). Differentiated daughter cell shows strong nuclear Prospero and is indicated by the arrow. …
Figure 2—figure supplement 1
Nuclear Prospero levels are lower in stage 15 NB3-3T than in differentiating GMCs.
(A) Confocal images of a wild type NB3-3T (blue circle, identified by Eg-GFP and Dpn) and its adjacent GMC (yellow circle, identified by Eg-GFP and its smaller size) in stage 15 embryos. Scale bar: …
Figure 3
Prospero is required for neuroblast quiescence.
(A–B) Wild type and prospero mutant (pros17) stage 16 embryos. Parental neuroblasts (NB) stain for Deadpan and mγ-LacZ, whereas ectopic ‘de-differentiated’ neuroblasts stain for Deadpan but not …
Figure 4 with 2 supplements
Prospero is sufficient to induce neuroblast quiescence.
(A–C) Transient overexpression of nuclear Prospero in neuroblasts (worniu-gal4 UAS-HA:prospero tub-gal80ts) results in neuroblast quiescence (A) or differentiation (B). Third-instar larvae were …
Figure 4—figure supplement 1
Low nuclear Prospero induces neuroblast quiescence, whereas high Prospero induces neuroblast differentiation.
(A) Two levels of Prospero were generated by increasing time of misexpression (4 or 12 hr) with one copy of UAS-HA:prospero transgene. Whereas 2 hr can induce neuroblast quiescence (Figure 4), 4 hr …
Figure 4—figure supplement 2
Prospero is sufficient to induce embryonic neuroblast quiescence.
(A–G) Transient overexpression of nuclear Prospero in neuroblasts (worniu-gal4 UAS-HA:prospero tub-gal80ts) induces neuroblast cell cycle arrest. Embryos were shifted from 22°C to 30°C for 1 hr to …
