(A) PCR amplifications of portions of the GH25 muramidase domain in the indicated taxa. All amplifications were Sanger sequenced to confirm integration. Primers used are listed in Table S3. Abbreviations: Sb: S. braunii, Sm: S. moellendorffii, Su: S. uncinata, Ssa: S. sanguinolenta, Sst: S. stauntoniana, Sl: S. lepidophylla, E: East Pacific Rise, L: Lao Spreading Center, M: Mid-Atlantic Ridge, Pu: Pleotrichophorus utensis, Aa: Artemisaphis artemisicola, Ue: Uroleucon erigeronensis, Av: Aphis varians, Ap: Acyrthosiphon pisum, Al: Aphis lupini, Cs: Cedoaphis sp., As: Aphthargelia symphoricarpi, Bs: Braggia sp., - denotes water only control. (B) World map with approximate locations of A. boonei field samples. Those that tested positive for the GH25 muramidase domain are indicated by green stars and those that tested negative are indicated by red stars. Map is a public domain image from Wikimedia Commons.