Unsupervised segmentation in biological and non-biological images is only partially resolved. Segmentation either requires arbitrary thresholds or large teaching datasets. Here, we propose a spatial autocorrelation method based on Local Moran’s I coefficient to differentiate signal, background, and noise in any type of image. The method, originally described for geoinformatics, does not require a predefined intensity threshold or teaching algorithm for image segmentation and allows quantitative comparison of samples obtained in different conditions. It utilizes relative intensity as well as spatial information of neighboring elements to select spatially contiguous groups of pixels. We demonstrate that Moran’s method outperforms threshold-based method in both artificially generated as well as in natural images especially when background noise is substantial. This superior performance can be attributed to the exclusion of false positive pixels resulting from isolated, high intensity pixels in high noise conditions. To test the method’s power in real situation, we used high power confocal images of the somatosensory thalamus immunostained for Kv4.2 and Kv4.3 (A-type) voltage-gated potassium channels in mice. Moran’s method identified high-intensity Kv4.2 and Kv4.3 ion channel clusters in the thalamic neuropil. Spatial distribution of these clusters displayed strong correlation with large sensory axon terminals of subcortical origin. The unique association of the special presynaptic terminals and a postsynaptic voltage-gated ion channel cluster was confirmed with electron microscopy. These data demonstrate that Moran’s method is a rapid, simple image segmentation method optimal for variable and high noise conditions.

Deciding between a pair of familiar items is thought to rely on a comparison of their subjective values. When the values are similar, decisions take longer, and the choice may be inconsistent with stated value. These regularities are thought to be explained by the same mechanism of noisy evidence accumulation that leads to perceptual errors under conditions of low signal to noise. However, unlike perceptual decisions, subjective values may vary with internal states (e.g. desires, priorities) that change over time. This raises the possibility that the apparent stochasticity of choice reflects changes in value rather than mere noise. We hypothesized that these changes would manifest in serial dependencies across decision sequences. We analyzed data from a task in which participants chose between snack items. We developed an algorithm, Reval, that revealed significant fluctuations of the subjective values of items within an experimental session. The dynamic values predicted choices and response times more accurately than stated values. The dynamic values also furnished a superior account of the BOLD signal in ventromedial prefrontal cortex. A novel bounded-evidence accumulation model with temporally correlated evidence samples supports the idea that revaluation reflects the dynamic construction of subjective value during deliberation, which in turn influences subsequent decisions.