1. Neuroscience

Regulation of food intake by mechanosensory ion channels in enteric neurons

  1. William H Olds
  2. Tian Xu  Is a corresponding author
  1. Howard Hughes Medical Institute, Boyer Center for Molecular Medicine, Yale University School of Medicine, United States
  2. Fudan-Yale Center for Biomedical Research, Fudan University, China
https://doi.org/10.7554/eLife.04402
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  1. William H Olds
  2. Tian Xu
(2014)
Regulation of food intake by mechanosensory ion channels in enteric neurons
eLife 3:e04402.
https://doi.org/10.7554/eLife.04402
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Figure 1
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Modulating activities of Drosophila PENs causes metabolic defects.

(A) Enteric neural projections of Gal4 lines tested (red, phalloidin; green, UAS-mCD8::GFP) and their diagram (B). GMR51F12-Gal4 neurons project to the foregut, anterior midgut and crop. GMR48A05-Gal4 neurons project to the proventriculus and anterior midgut. Both HGN1-Gal4 and Ilp7-Gal4 drive expression in the neurons projecting to the posterior midgut, hindgut pylorus, anterior hindgut, rectal pylorus and the rectum. Pr, Proventriculus; C, Crop; Py, Pylorus; RP, Rectal Pylorus; R, Rectum; VNC, Ventral Nerve Cord. The effects of activating (C) or inactivating (D) enteric neurons on hemolymph glucose (GMR51F12-Gal4, GMR48A05-Gal4, Ilp7-Gal4, or HGN1-Gal4; UAS-TRPA1 or UAS-shiTS1) (n = 6–10 replicates of 10 flies). (E) The effect of silencing the PENs in starvation conditions (Ilp7-Gal4 or HGN1-Gal4; UAS-shiTS1) (n = 6–9 replicates of 10 flies). * = p < 0.05, compared to corresponding UAS and Gal4 control. Significances indicated are based on ANOVA and Tukey post-hoc test. Data represent the average ± s.e.m. of the results obtained.

https://doi.org/10.7554/eLife.04402.003
Figure 2
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PPK1 functions in Drosophila PENs to regulate feeding.

(A–B) Results of capillary feeding assays by either inactivating (A, Ilp7-Gal4 or HGN1-Gal4; UAS-shiTS1) or activating (B, Ilp7-Gal4 or HGN1-Gal4; UAS-TRPA1) the PENs (n = 4–8 replicates). (C) Outside and inside views of the hindgut (red, phalloidin, muscle) with posterior enteric neuron projections (green, 22C10). (D) PPK1 expresses in the PENs projecting to the hindgut pylorus (left) and rectum (right) (PPK1-Gal4;UAS-mCD8::GFP). (E) The effect of PPK1 knock-down on food intake (Ilp7-Gal4 or HGN1-Gal4, UAS-PPK1-RNAi#1 or UAS-PPK1-RNAi#2) (n = 3–8 replicates). (F) Food intake results for PPK1 deficiency (dfb88h49/dfA400) and rescued animals (dfb88h49/dfA400; Ilp7-Gal4, UAS-PPK1) (n = 4–7 replicates). (G) Food intake results when PPK1 is inhibited using benzamil in wild-type or Ilp7 > PPK1 RNAi #1 flies (n = 8–10 replicates). * = p < 0.05, compared to corresponding UAS and Gal4 control or indicated controls. Significances indicated are based on ANOVA and Tukey post-hoc test. Data represent the average ± s.e.m. of the results obtained.

https://doi.org/10.7554/eLife.04402.004

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  1. William H Olds
  2. Tian Xu
(2014)
Regulation of food intake by mechanosensory ion channels in enteric neurons
eLife 3:e04402.
https://doi.org/10.7554/eLife.04402