(a) Five representative YcgC homologs with protein sequence homology ranging from low to high. (b) Amino acid sequence homology analysis between YcgC and five selected YcgC homologs from other bacteria. The consensus strength among the six homologous proteins at each amino acid position of YcgC is indicated with colored bars. Red, orange, green, light blue, dark blue, and blank bars represent 100, 80, 60, 40, 20, and 0% consensus strength, respectively. (c,d) Changes in global E. coli acetylation profiles upon induction of the five YcgC homologs. The five selected YcgC homologs were cloned, transformed into E. coli, and induced to overexpress. Global acetylation profiles of each induced strain were detected with a pan monoclonal antibody (Cell Signaling, #9441) and a pan polyclonal antibody (PTM-Biolabs, PTM-105), as shown in c and d, respectively. WT E. coli cells were also processed in parallel as a comparison. An antibody against myelin basic protein was used as a loading control. WT: Wild type.