(A) Summary of genome-wide distribution of G9a, ZNF644 and WIZ in different regions. Y-axes: percentage of each region in the genome. (B) Venn diagram shows a significant overlap between G9a, ZNF644 and WIZ enriched peaks. (C) The G9a-enriched peaks were bound with ZNF644 and/or WIZ, especially in promoter region. (D) G9a, ZNF644 and WIZ ChIP-seq read counts in 100-bp window were plotted against the distance (−2 kb, +2 kb) from the center of G9a enriched regions in promoter region. Y-axes: mean tag density. (E) ChIP-seq results show the co-occupancy of ZNF644, WIZ and G9a at CWH43, DIP2C and ROCK1 loci. (F) ZNF644 and G9a are co-localized at the promoter regions of CACNA2D1, ANKRD26P1, USP14 and HCN1. (G) WIZ and G9a are co-localized at the promoter regions of PARD3, ABCA13, SENP5 and NRXN3. (H) The consensus DNA-binding motif of ZNF644 is analyzed according to ChIP-seq result. The binding sequences in CACNA2D1, ANKRD26P1, USP14 and HCN1 loci are shown in red. (I) The specific DNA binding sequence of WIZ is obtained according to the ChIP-seq results, and is confirmed at PARD3, ABCA13, SENP5 and NRXN3 loci. (J) Both ZNF644 and WIZ-binding sequences are identified at CWH43, DIP2C and ROCK1 loci, which are co-occupied by ZNF644 and WIZ.