RT-qPCR analysis of indicated HCMV genes expression in MRC-5 (A) or cord blood CD34+ (B, C, D, E) cells infected with the TB40-E strain 3 days after (A, B, C) or 7 days prior to (D, E) being transduced or not (NT) with lentivectors expressing (shKAP1) or not (empty) a small hairpin RNA against Kap1, sorting CD34+ cells for GFP and CD34 expression. RT-qPCRs were performed 7 days after HCMV infection (A and B) or 7 days after lentiviral transduction (D and E). Results are presented as average of fold change expression vs NT after GAPDH and β-2M normalization (n = 4, *p < 0.05, **p < 0.01, error bars as s.d.). (C and E) HCMV production in CD34+ cells was quantified by plaque assay on MRC-5 cells. Results are presented as average of PFU/ml (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, error bars as s.d.). See Figure 1—figure supplements 3, 4 for all individual experiments.