(A) To assess differential connectivity of PRC vs PHC with the EC, voxelwise paired-sample t-tests were performed on the normalized single-subject beta maps (resulting from seed-to-voxel connectivity analyses). Significant clusters for Exp. 1 are shown for two coronal sample slices (Z > 2.3. pcluster < 0.05, NExp. 1 = 15) at the level of the anterior (1) and posterior (2) hippocampal head. (B) To visualize the 3-dimensional geometry of connectivity, the connectivity preference with PRC vs PHC of each EC voxel was plotted along the x-, y-, and z-axis (red: TPRC > PHC > 0, blue: TPHC > PRC > 0). Axes terminology is relative to the long-axis of the hippocampus. (C) Classification of PRC vs PHC connectivity preference was tested across subjects based on the x-y-z coordinate of an EC voxel. Multivariate classification (support vector machine; leave-one-subject-out cross-validation) was significant across both data sets (p < 0.001; accuracies: Exp. 1: left: 62%, right: 60%, Exp. 2: left: 67%, right: 57%), which confirms a spatial dissociation of entorhinal connectivity with PRC vs PHC. Predicted clusters are color-coded in red vs. blue, bright regions denote high consistency of the classifier (accuracy for each voxel across subjects). Results are shown for Exp. 1. See also Figure 2—figure supplement 1 for 3D plots of Exp. 2 and for unilateral seeds of Exp. 1.