(A) Confocal microscopy of thick lymph node (LN) sections prepared from mice that had received adoptively transferred B cells (previous day), injected with fluorescently labeled gp120, and …
(A) Confocal microscopy image of FACS sorted SIGN-R1+ macrophage 2 hr after sorting. Differential interference contrast (DIC) visualized the cell body and nucleus and was used as a background …
Flow cytometric analysis of cells prepared from the inguinal LNs of interferon-γ-eYFP reporter mice administered gp120 near the tail base at 0, 3 and 6 hr prior to collection. eYFP vs SIGN-R1 …
(A) Flow cytometric analysis of various cellular populations prepared from the inguinal LNs of interferon-γ-eYFP reporter mice administered gp120 near the tail base, 0 (shaded), 3 (shaded, darker) …
(A) Confocal microscopy of a thick LN section from a mouse previously injected with fluorescent gp120 and immunostained for B220 and CD3. Scale bar is 30 μm. Boxed areas in left image were enlarged …
Confocal microscopy of a thick LN section from a mouse previously injected with fluorescent gp120 and immunostained for CD169 and CD11c. The image is centered over an IFC. Scale bar is 30 μm. Boxed …
Confocal microscopy of human frozen LN sections overlaid with fluorescent gp120 and immunostained for the indicated markers. (A) Section was stained with CD19 (blue) and CD4 (gray). Scale bar is 400 …
(A) Intravital TP-LSM images of the inguinal LN 8 min after injection of fluorescent gp120 and 30 min after CD169. Yellow circles indicate sinus macrophages (upper panel) or network cells in the IFC …
(A, B) Confocal microscopy of a thick LN section immunostained for CD4, white, following injection of NP-PE, red, fluorescent gp120, green, and fluorescent HEL, blue, near the inguinal LN. Three …
(A) Intravital TP-LSM image of the inguinal lymph following the injection of differentially labeled R66M gp120 expressed in either 293F or CHO-S cells. Scale bars are 100 μm. (B) A z-projection (50 …
Confocal microscopy of thick LN sections overlaid with fluorescent gp120, which is treated with PNGase F or not, and immunostained for the indicated markers. (A) LN section image (sagittal, tiled) …
Confocal microscopy of thick LN sections prepared from mice that had been injected with biotinylated soluble trimeric gp120. Biotinylated gp120 was detected by AlexaFluor 488 conjugated …
(A) Flow cytometry to evaluate the binding of labeled R66M gp120 to follicular and marginal zone B cells from either b12 HL or b12 L B cells. Light gray line is background fluorescence. MFI-mean …
(A) Intravital TP-LSM images of b12 HL, red, and WT B, blue, cells in the inguinal LN at 3, 35, and 63 min following injection of fluorescent gp120, green, near the inguinal LN. Blood vessels were …
(A) Six tracks, red lines, and displacements, yellow or cyan arrows, of b12 HL B cells were superimposed on 3-D reconstruction image of gp120 loaded IFC network cells, green. 3-D reconstruction …
Images from two focal planes separated by 30 μm and located over the IFC channel of a mouse inguinal lymph node (LN). The mouse had previously been injected with fluorescent CD169 antibody, red, …
An image sequence of a 30 μm z-projection was acquired from a LysM-EGFP mouse, which had previously received by adoptive transfer both B cells, blue, and CD4 T cells, purple. Host endogenous …
An image sequence of a 20 μm z-projection was acquired from the inguinal LN following nearby injection of fluorescent gp120, red, and SIGN-R1, green, antibody (left). Track and displacement (yellow …
An image sequence of a 20 μm z-projection was acquired from the inguinal LN of a mouse, which had fluorescent gp120 (green) delineated IFC network cells. Fluorescently labeled b12 HL B cells (red) …
An image sequence of a 25 μm z-projection was acquired from the inguinal LN of a mouse, which has fluorescent gp120 (green) delineated IFC network cells. b12 HL B cells (blue) were labeled with …
An image sequence of a 20 μm z-projection was acquired from inguinal LN of mouse, which had received by adoptive transfer the previous day b12 HL B cells, red, and wild type B cells, blue. Evans …