(A) Whole-mount acetylated tubulin (AcTub) staining. Scale bars: 500 μm. Inset shows depth-coded projection of AcTub staining. Superficial structures are in blue and deeper structures are in red. …
(A) Cartoon shows previous segmentation model of the protonephridial tubule and expression map of slc genes along the protonephridial tubule. (B–G) Representative images show expression domains of …
Planarian homologs are colored in red.
Planarian homologs are colored in red.
Planarian homologs are colored in red.
Planarian homologs are colored in red.
Planarian homologs are colored in red.
Planarian homologs are colored in red.
Whole-mount expression patterns of slc genes by in situ hybridization (NBT/BCIP development). Scale bars: 500 μm.
Whole-mount expression patterns of slc genes by in situ hybridization (NBT/BCIP development). Scale bars: 500 μm.
Whole-mount expression patterns of slc genes by in situ hybridization (NBT/BCIP development). Scale bars: 500 μm.
Whole-mount expression patterns of slc genes by in situ hybridization (NBT/BCIP development). Scale bars: 500 μm.
Whole-mount expression patterns of slc genes by in situ hybridization (NBT/BCIP development). Scale bars: 500 μm.
Whole-mount expression patterns of slc genes by in situ hybridization (NBT/BCIP development). Scale bars: 500 μm.
Fluorescent overlay of indicated gene (in red) (A-F) with PT2 and PT3 marker (slc6a-13), DT marker (CAVII-1) and AcTub staining. Images are maximum projections of confocal Z-sections. Scale bars: 50 …
(A) Fluorescent overlay of indicated gene (in red) with PT1 and PT2 marker (CUBN1), PT2 and PT3 marker (slc6a-13) and AcTub staining. (B–D) Fluorescent overlay of indicated gene (in red) with PT2 …
Fluorescent overlay of indicated gene (in red) with PT2 and PT3 marker (slc6a-13), DT marker (CAVII-1) and AcTub staining. Images are maximum projections of confocal Z-sections. Scale bars: 50 μm. A …
Fluorescent overlay of indicated gene (in red) (A-C) with PT2 and PT3 marker (slc6a-13), DT marker (CAVII-1) and AcTub staining. Images are maximum projections of confocal Z-sections. Scale bars: 50 …
Fluorescent overlay of indicated gene (in red) (A-G) with PT marker (slc6a-13 or CUBN1), DT marker (CAVII-1) or CD marker (slc12a-1 or slc24a-9) together with AcTub staining. Images are maximum …
Fluorescent overlay of indicated gene (in red) (A-P) with PT2 and PT3 marker (slc6a-13), DT marker (CAVII-1) or CD marker (slc24a-9) together with AcTub staining. Images are maximum projections of …
Fluorescent overlay of indicated gene (in red) (A-B) with PT2 and PT3 marker (slc6a-13), DT marker (CAVII-1) and AcTub staining. Images are maximum projections of confocal Z-sections. Scale bars: 50 …
(A) Tables summarize expression domains of selected slc genes in planarian protonephridia and rodent metanephros. Cartoons showing segmental organization of planarian protonephridia and rodent …
(A) Whole-mount expression patterns of indicated marker genes by in situ hybridization (NBT/BCIP development). Scale bars: 500 μm. (B) Fluorescent overlay of indicated gene (red) with flame cell …
(A) Cartoon showing the glomerular filtration barrier. Top: A schematic view of the podocyte. The podocyte wraps around the capillary wall on the outer surface of the glomerular basement membrane …
(A, B) Fluorescent overlay of flame cell markers (CXCRL and EGFR-5) with AcTub staining in intact (A) and regenerating (B) Control(RNAi) and NPHS1-6(RNAi). Images are maximum projections of confocal …
TEM images showing cross-section through a flame cell in regenerating Control(RNAi) and NPHS1-6(RNAi) animals. Inset shows high magnification of filtration diaphragm. Scale bar: 1 μm.
(A) Protonephridial defects in NPHP5(RNAi), NPHP6(RNAi) and NPHP8(RNAi) animals. Top panel: live images show edema in intact RNAi animals. Scale bars: 500 μm; middle panel: monochrome showing AcTub …
(A) Homology analysis of planarian nephrocystins. Domains predicted by SMART for planarian and human proteins. Best reciprocal BLAST hits in human, C. elegans, and fly refseq protein database. (B) …
(A) Fluorescent overlay of lumen marker with PT2 and PT3 marker slc6a-13 and nuclei (DAPI) in Control(RNAi) and NPHP8(RNAi) animals. Scale bars: 25 μm. (A) Fluorescent overlay of PT marker (slc6a-13)…
TEM images showing cross-section through a tubule of protonephridia in indicated RNAi animals. Inset in red box shows abnormally localized basal body in indicated RNAi animals. Inset in green box …
(A) BrdU pulse-chase experiment shows the presence of diving protonephridial progenitors in the proximity of protonephridial tubule in Control(RNAi) and NPHP8(RNAi) animals. Yellow arrowhead …
(A) Left: Whole-mount expression patterns of POU2/3 by in situ hybridization. Scale bars: 500 μm. Right: Fluorescent overlay of POU2/3 with DT marker (CAVII-1) and AcTub. Scale bar: 50 μm. (B) Left: …
(A, B) Left panel: whole-mount expression patterns of pax6A (A) and HNF4 (B) by in situ hybridization. Scale bars: 500 μm; Right panel: fluorescent overlay of (A) pax6A and (B) HNF4 with pan stem …
(A) Quantification of mitoses in Control(RNAi) and NPHP8(RNAi) animals. Experimental paradigm is described in Figure 5F. The time point in the bar graph indicates the number of the day after the …
(A) Series of live images show gliding mobility in Control(RNAi) and NPHP8(RNAi) animals. Yellow dot line provides a spatial reference to illustrate progress of animal. Scale bar: 1 mm. (B) …
(A) Quantification of translocation speed in indicated RNAi animals. Error bar, SD; *** p < 0.001 vs control. (B) Schematic drawing shows the structure of 9 + 2 motile cilia in planarians. Right …
(A) Quantification of average area of each slc6a13+ tubule (right) in Control(RNAi), IFT88(RNAi) and NPHP8(RNAi) animals to evaluate the severity of protonephridial cystogenesis. (B) Quantification …
Serial TEM images showing the protonephridial collecting duct connected to the dorsal epithelia.
Serial TEM images showing flame cell in Control(RNAi) animal.
Serial TEM images showing flame cell in NPHS1-6(RNAi) animal.
Serial images show beating of the cilia along the lateral body edge of the planarian head region in Control(RNAi) animal.
Serial images show beating of the cilia along the lateral body edge of the planarian head region in DNAHβ-1(RNAi) animal.
Serial images show beating of the cilia along the lateral body edge of the planarian head region in LRRC50(RNAi) animal.
Summary information of the planarian homologs of slc genes.
Expression domains of slc genes in the planarian protonephridia.
Expression domains of slc genes in the rodent metanephros.
Summary information of the planarian homologs of human kidney disease genes.