Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche
- Stowers Institute for Medical Research, United States
- University of Kansas School of Medicine, United States
- Tsinghua University, China
Figures
Figure 1 with 1 supplement
Canonical Wnt signaling in ISCs promotes germ cell differentiation.
(A) The Drosophila germarium dividing into three regions 1, 2a, 2b and 3. Abbreviations: TF-terminal filament; CPC-cap cell; ISC-inner germarial sheath cell; FC-follicle cell; GSC-germ line stem …
Figure 1—figure supplement 1
Wnt receptors FZ and FZ2 function redundantly in ISCs to promote germ cell differentiation.
Broken ovals highlight cap cells and GSCs, while arrowheads denote spectrosomes in CBs (A–C, E). (A–D) fzKD (B) and fz2KD (C) germaria contain 0 and 1 CB, respectively, in comparison with the gfpKD …
Figure 2
Wnt signaling is required in adult ISCs to promote germ cell differentiation.
Broken ovals highlight germ line stem cells (GSCs), whereas arrowheads indicate cystoblasts (CBs). Two experimental regimens 25–25 and 25–29 mean the females cultured under 25°C or 29°C for one …
Figure 3 with 1 supplement
Wnt signaling maintains the differentiation niche by promoting cell proliferation and survival.
Cap cells are highlighted by broken ovals. (A) The control germarium contains PZ1444-labeled cap cells and ISCs (one by arrow). (B–D) The armKD (B) and dshKD (D) germaria maintain zero and one ISC …
Figure 3—figure supplement 1
Hyperactive Wnt signaling increases the ISC population.
Cap cells are highlighted by ovals, while some of the ISCs are denoted by arrowheads. (A) In the control germarium, PZ1444 labels cap cells and ISCs. (B–D) The axnKD (B) and sggKD (C, D) germaria …
Figure 4
Wnt signaling in ISCs prevents BMP signaling in the differentiated germ cell zone and maintains long ISC cellular processes.
Ovals indicate GSCs, whereas arrows in A-F′ denote CBs or CB-like single germ cells. (A′–F′) Images only show green fluorescence of (A–F). (A, A′) In the control germarium, GSCs are positive for …
Figure 5
Piwi mRNA and protein expression remain normally expressed in the Wnt signaling-defective ISCs.
PZ1444 is used to highlight ISCs. (A) The control germarium shows that ISCs (arrow) express more Piwi proteins than cap cells (oval) and underneath germ cells. (B, C) The remaining armKD (B) and dshK…
Figure 6 with 2 supplements
Wnt signaling maintains the reduced redox state in ISCs, promoting germ cell differentiation.
(A) RNA-seq results of the purified ISCs show that GstD2, GstD4, GstD10, and GstE3 mRNA expression levels are significantly lower in axn-overexpressing or dskKD ISCs than the control ISCs. In B–B′″, …
Figure 6—figure supplement 1
Wnt signaling maintains the reduced redox state in ISCs.
(A) The control ovariole exhibit low DHE staining in the germarial region and gradually upregulates DHE staining in differentiated germ cells in egg chambers. B′–F′ only show DHE staining in B–F. In …
Figure 6—figure supplement 2
Reduced redox state is important for ISC maintenance.
In A–F, broken ovals highlight cap cells and GSCs, whereas arrowheads point to CBs. (A–G) c587-directed overexpressing GST2 (A), CAT (B), or SOD1 (C) in ISCs does not affect GSC maintenance and germ …
Figure 7
Wnt2 and Wnt4 function redundantly in ISCs to promote germ cell differentiation.
(A) RNA-seq results show that Wnt2 and Wnt4 are highly expressed in the purified ISCs in comparison with Wnt5 and Wnt6. In B–C′ and E–H, cap cells and GSCs are highlighted by ovals, CBs are …
