ANGPTL4 mediates shuttling of lipid fuel to brown adipose tissue during sustained cold exposure
- Wageningen University, Netherlands
- University of Hamburg, Germany
- University of California, Los Angeles, United States
- Leiden University, Netherlands
- Maastricht University, Netherlands
- European Society for Medical Oncology, Netherlands
- Umeå University, Sweden
- Leiden University Medical Center, Netherlands
Abstract
Brown adipose tissue (BAT) activation via cold exposure is increasingly scrutinized as a potential approach to ameliorate cardio-metabolic risk. Transition to cold temperatures requires changes in the partitioning of energy substrates, re-routing fatty acids to BAT to fuel non-shivering thermogenesis. However, the mechanisms behind the redistribution of energy substrates to BAT remain largely unknown. Angiopoietin-like 4 (ANGPTL4), a protein that inhibits lipoprotein lipase (LPL) activity, is highly expressed in BAT. Here, we demonstrate that ANGPTL4 is part of a shuttling mechanism that directs fatty acids derived from circulating triglyceride-rich lipoproteins to BAT during cold. Specifically, we show that cold markedly down-regulates ANGPTL4 in BAT, likely via activation of AMPK, enhancing LPL activity and uptake of plasma triglyceride-derived fatty acids. In contrast, cold up-regulates ANGPTL4 in WAT, abolishing a cold-induced increase in LPL activity. Together, our data indicate that ANGPTL4 is an important regulator of plasma lipid partitioning during sustained cold.
Article and author information
Author details
Reviewing Editor
- Michael Czech, University of Massachusetts Medical School, United States
Ethics
Animal experimentation: This study was performed in accordance with Directive 2010/63/EU from the European Union. All of the animals were handled according to protocols approved by the Animal Ethics Committees of Wageningen University and Hamburg University (2013007.d, 2013100.b and 34/12).
Human subjects: All subjects signed an informed consent for the study protocol, which was approved by the institutional review board of Maastricht University Medical Centre.
Version history
- Received: April 29, 2015
- Accepted: October 16, 2015
- Accepted Manuscript published: October 17, 2015 (version 1)
- Version of Record published: December 30, 2015 (version 2)
Copyright
© 2015, Dijk et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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ANGPTL4 mediates shuttling of lipid fuel to brown adipose tissue during sustained cold exposure
eLife 4:e08428.
https://doi.org/10.7554/eLife.08428
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