Muscle niche-driven Insulin-Notch-Myc cascade reactivates dormant Adult Muscle Precursors in Drosophila

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Cite this article as: eLife 2015;4:e08497 doi: 10.7554/eLife.08497

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Abstract

How stem cells specified during development keep their non-differentiated quiescent state, and how they are reactivated, remain poorly understood. Here we applied a Drosophila model to follow in vivo behavior of Adult Muscle Precursors (AMPs), the transient fruit fly muscle stem cells. We report that emerging AMPs send out thin filopodia that make contact with neighboring muscles. AMPs keep their filopodia-based association with muscles throughout their dormant state but also when they start to proliferate, suggesting that muscles could play a role in AMP reactivation. Indeed, our genetic analyses indicate that muscles send inductive dIlp6 signals that switch the Insulin pathway ON in closely associated AMPs. This leads to the activation of Notch, which regulates AMP proliferation via dMyc. Altogether, we report that Drosophila AMPs display homing behavior to muscle niche and that the niche-driven Insulin-Notch-dMyc cascade plays a key role in setting the activated state of AMPs.

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Rajaguru Aradhya

Rockefeller Research Laboratories, Sloan-Kettering Institute, New York, United States

Competing interests
The authors declare that no competing interests exist.
Monika Zmojdzian

Génétique Reproduction et Développement, Institut national de la santé et de la recherche médicale, Clermont Université, Clermont-Ferrand, France

Competing interests
The authors declare that no competing interests exist.
Jean Philippe Da Ponte

Génétique Reproduction et Développement, Institut national de la santé et de la recherche médicale, Clermont Université, Clermont-Ferrand, France

Competing interests
The authors declare that no competing interests exist.
Krzysztof Jagla

Génétique Reproduction et Développement, Institut national de la santé et de la recherche médicale, Clermont Université, Clermont-Ferrand, France

For correspondence
christophe.jagla@udamail.fr

Competing interests
The authors declare that no competing interests exist.

Reviewing Editor

Margaret Buckingham, Institut Pasteur, France

Publication history

Received: May 3, 2015
Accepted: October 28, 2015
Accepted Manuscript published: December 9, 2015 (version 1)
Version of Record published: January 29, 2016 (version 2)

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.