1. Developmental Biology
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Developmental compartments in the larval trachea of Drosophila

  1. Prashanth R Rao
  2. Li Lin
  3. Hai Huang
  4. Arjun Guha
  5. Sougata Roy
  6. Thomas B Kornberg  Is a corresponding author
  1. University of California, San Francisco, United States
  2. PharmaLex GmbH, Germany
  3. Boston University, United States
  4. University of Maryland, College Park, United States
Research Article
  • Cited 8
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Cite this article as: eLife 2015;4:e08666 doi: 10.7554/eLife.08666

Abstract

The Drosophila tracheal system is a branched tubular network that forms in the embryo by a post-mitotic program of morphogenesis. In third instar larvae (L3), cells constituting the second tracheal metamere (Tr2) reenter the cell cycle. Clonal analysis of L3 Tr2 revealed that dividing cells in the dorsal trunk, dorsal branch and transverse connective branches respect lineage restriction boundaries near branch junctions. These boundaries corresponded to domains of gene expression, for example where cells expressing Spalt, Delta and Serrate in the dorsal trunk meet vein-expressing cells in the dorsal branch or transverse connective. Notch signaling was activated to one side of these borders and was required for the identity, specializations and segregation of border cells. These findings suggest that Tr2 is comprised of developmental compartments and that developmental compartments are an organizational feature relevant to branched tubular networks.

Article and author information

Author details

  1. Prashanth R Rao

    Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Li Lin

    PharmaLex GmbH, Mannheim, Germany
    Competing interests
    The authors declare that no competing interests exist.
  3. Hai Huang

    Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Arjun Guha

    Pulmonary Center, Boston University School of Medicine, Boston University, Boston, United States
    Competing interests
    The authors declare that no competing interests exist.
  5. Sougata Roy

    Department of Molecular and Cellular Biology, University of Maryland, College Park, College Park, United States
    Competing interests
    The authors declare that no competing interests exist.
  6. Thomas B Kornberg

    Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States
    For correspondence
    tkornberg@ucsf.edu
    Competing interests
    The authors declare that no competing interests exist.

Reviewing Editor

  1. Ginés Morata, Universidad Autónoma de Madrid, Spain

Publication history

  1. Received: May 12, 2015
  2. Accepted: October 21, 2015
  3. Accepted Manuscript published: October 22, 2015 (version 1)
  4. Version of Record published: January 11, 2016 (version 2)

Copyright

© 2015, Rao et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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