Bidirectional interactions between indomethacin and the murine intestinal microbiota
- University of Pennsylvania, United States
- Food and Drug Administration, United States
Figures
Figure 1
Geographic heterogeneity of basal intestinal microbiota composition along the intestine in mice.
Bacterial communities colonized in the mouse intestine were profiled using 16S rRNA gene sequencing and analyzed using QIIME (Caporaso et al., 2010b). (A) Principal coordinates analysis (PCoA) of …
Figure 2 with 5 supplements
Indomethacin induces changes in microbial composition along the intestine in mice.
Bacterial load in samples were inferred from 16S rRNA gene quantitative PCR. Bacterial communities colonized in the mouse intestine were profiled using 16S rRNA gene sequencing and analyzed using …
Figure 2—figure supplement 1
Indomethacin induces small intestinal damage in C57BL/6 mice.
Representative sections of small intestinal injuries 24 hr after 10 mg/kg indomethacin treatment, including macroscopic views (left) and hematoxylin and eosin (H&E) staining (right). Macroscopically …
Figure 2—figure supplement 2
Inhibitory effects of acute indomethacin treatment on COX-1 and COX-2 in C57BL/6 mice.
Mice were administered by gavage with or without 10 mg/kg indomethacin (red) or PEG400 (blue) and urine were collected for the analysis of prostanoid metabolites. PGD-M (A), PGE-M (B), PGI-M (C), …
Figure 2—figure supplement 3
C57BL/6 mice are systemically and locally exposed to indomethacin.
Mice were administered by gavage 10 mg/kg indomethacin in PEG400 (red) or PEG400 alone (blue). Urine, feces, plasma, and intestines were collected from mice at 6 hr after drug administration. …
Figure 2—figure supplement 4
Inhibitory effects of chronic indomethacin treatment on COX-1 and COX-2 in C57BL/6 mice.
Mice were receiving control diet (black) or indomethacin diet (20 ppm, red) for 7 days and urine were collected for the analysis of prostanoid metabolites. PGD-M (A), PGE-M (B), PGI-M (C), and Tx-M …
Figure 2—figure supplement 5
Chronic indomethacin treatment induces changes in microbial composition along the intestine in mice.
Bacterial communities colonized in the mouse intestine were profiled using 16S rRNA gene sequencing and analyzed using QIIME (Caporaso et al., 2010b). Observed Species (A) and Shannon Index (B) are …
Figure 3 with 3 supplements
Indomethacin induces longitudinal changes in fecal microbiota composition.
Microbiota composition in fecal pellets before (0 hr) and after (6 hr) treatment with or without indomethacin or PEG400 is analyzed by 16S rRNA gene profiling, including sequencing and quantitative …
Figure 3—figure supplement 1
Longitudinal effects of acute indomethacin treatment in fecal microbiota composition.
Microbiota composition in fecal pellets before (0 hr, black) and after (6 hr, blue) treatment with or without indomethacin or PEG400 is analyzed by 16S rRNA gene profiling. (A) The relative …
Figure 3—figure supplement 2
Longitudinal effects of chronic indomethacin treatment in fecal microbiota composition.
Fecal microbiota composition before (day 0) and after (day 8) treatment in mice receiving control or indomethacin diet is analyzed by 16S rRNA gene profiling. (A) Observed species (left) and Shannon …
Figure 3—figure supplement 3
Longitudinal effects of chronic indomethacin treatment on genera abundance in fecal microbiota.
Fecal microbiota composition before (day 0) and after (day 8) treatment in mice receiving control or indomethacin diet is analyzed by 16S rRNA gene profiling. Indomethacin induced increases in the …
Figure 4 with 1 supplement
Microbiota-depletion with antibiotics alters the pharmacokinetics of indomethacin in mice.
Mice were subjected to control water (Con) or antibiotic cocktail (Abx, neomycin and vancomycin) for 5 days (blue-shaded area). Upon the cessation of 5-day treatment, mice were administered by …
Figure 4—figure supplement 1
Antibiotic-treatment causes compositional changes in intestinal microbiota without affecting body weight, food intake, and water intake in C57BL/6 mice.
Mice were subjected to antibiotic water (Abx, neomycin and vancomycin) or control water (Con) for 5 days (blue-shaded) and the body weight, food intake, and water intake were monitored daily. Fecal …
Figure 5 with 1 supplement
Metabolism and efficacy of indomethacin in antibiotic-treated mice are altered.
Upon the cessation of 5-day treatment with antibiotic cocktail (Abx, neomycin and vancomycin) or control water (Con), mice were administered by gavage with 10 mg/kg indomethacin. Urine and feces …
Figure 5—figure supplement 1
β-glucuronidase catalyzes de-glucuronidation reaction.
Mice were administered by gavage 10 mg/kg indomethacin in PEG400 after 5 days of antibiotic treatment. Urine and feces were collected at indicated times for the analysis of glucuronidation by in …
