Auxiliary subunits of the CKAMP family differentially modulate AMPA receptor properties
- German Cancer Research Center, Germany
- German Center for Neurodegenerative Diseases, Germany
- Medical Faculty of Heidelberg University, Germany
- The Hebrew University-Hadassah Medical School, Israel
Figures
Figure 1 with 1 supplement
Identification and molecular characterization of CKAMP proteins.
(A) Schematic drawing of CKAMP proteins, depicting the signal peptide (SP), cysteines (C) of the cystine knot, transmembrane domain (TM) and PDZ type II motif (EVTV). ‘AI’ indicates “AMPAR …
Figure 1—figure supplement 1
Comparison of Shisa proteins and CKAMPs.
Alignment of protein sequences that correspond to Shisa and CKAMP genes. Note that Shisa1 was originally described for Xenopus laevis oocytes and does not have a mouse homolog. Intensity of the blue …
Figure 2 with 2 supplements
Expression pattern of CKAMPs and their interaction with AMPARs.
(A) Transcription of CKAMP genes as visualized by in situ hybridization of brain sections obtained from mice at different developmental ages. Abbreviations: CB - cerebellum, CX - cortex, HIP - …
Figure 2—figure supplement 1
Analysis of splice isoforms for CKAMP family members.
Splice variants for CKAMP52 and CKAMP59 (note that CKAMP39 has only one splice variant). Exons are numbered below the scheme of the spliced mRNA. For both CKAMPs, at least two splice isoforms are …
Figure 2—figure supplement 2
Analysis of expression for CKAMP family members.
Expression of novel CKAMP family members in different mouse tissues according to the BioGPS database. Note that a significant expression of CKAMP39 was found only in the olfactory bulb and …
Figure 3
Modulation of AMPAR-mediated currents in Xenopus laevis oocytes.
(A) Western blot analysis on flag-tagged CKAMPs in Xenopus laevis oocytes. CKAMP39, CKAMP44, CKAMP52 & CKAMP59 where injected at 1, 3, 5 & 10 ng/oocyte, respectively (UI-un-injected oocytes). (B) …
Figure 4
Influence of CKAMP proteins on glutamate and CTZ potency.
(A) Glutamate potency for GluA1 or GluA2(Q) expressed along with CKAMP39, CKAMP44 or CKAMP52 in Xenopus laevis oocytes. Responses to increasing concentration of glutamate were recorded in the …
Figure 5 with 1 supplement
Modulation of AMPAR-mediated currents in HEK293/T17 cells.
(A) Peak current amplitude, (B) deactivation rate (τdeact), (C) desensitization rate (τdes) and steady-state amplitude and (D) τrecovery of GluA1- and GluA2(Q)-mediated currents. Deactivation and …
Figure 5—figure supplement 1
Analysis of total and surface AMPAR expression in HEK293/T17 cells.
Example Western blot images (left) and quantification (right) of total AMPAR, surface AMPAR, and β-actin. GluA1 (A) or GluA2 (B) was expressed in HEK293/T17 cells along with CKAMP family members or …
Additional files
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Supplementary file 1
- https://doi.org/10.7554/eLife.09693.012
